Spidroin N-terminal domain forms amyloid-like fibril based hydrogels and provides a protein immobilization platform

Recombinant spider silk proteins (spidroins) have multiple potential applications in development of novel biomaterials, but their multimodal and aggregation-prone nature have complicated production and straightforward applications. Here, we report that recombinant miniature spidroins, and importantly also the N-terminal domain (NT) on its own, rapidly form self-supporting and transparent hydrogels at 37 °C. The gelation is caused by NT α-helix to β-sheet conversion and formation of amyloid-like fibrils, and fusion proteins composed of NT and green fluorescent protein or purine nucleoside phosphorylase form hydrogels with intact functions of the fusion moieties. Our findings demonstrate that recombinant NT and fusion proteins give high expression yields and bestow attractive properties to hydrogels, e.g., transparency, cross-linker free gelation and straightforward immobilization of active proteins at high density

Emergence Versus Self-Organisation: Different Concepts But Promising When Combined

A clear terminology is essential in every research discipline. In the context of ESOA, a lot of confusion exists about the meaning of the terms emergence and self-organisation. One of the sources of the confusion comes from the fact that a combination of both phenomena often occurs in dynamical systems. In this paper a historic overview of the use of each concept as well as a working definition, that is compatible with the historic and current meaning of the concepts, is given. Each definition is explained by supporting it with important characteristics found in the literature. We show that emergence and self-organisation each emphasise different properties of a system. Both phenomena can exist in isolation. The paper also outlines some examples of such systems and considers the combination of emergence and self-organisation as a promising approach in complex multi-agent systems