Analysis and quantification of diagnostic serum markers and protein signatures for Gaucher disease

FWN – Publicaties zonder aanstelling Universiteit Leide

Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards

Medical Biochemistr

Qualitative and quantitative proteomic profiling of cripto-1- embryonic stem cells by means of accurate mass LC-MS analysis

Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto-/- murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto-/- ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene.Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto-/- murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto-/- ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene. © 2009 American Chemical Society

Proteomic profiling of proliferating and differentiated neural mes-c-myc A1 cell line From mouse embryonic mesencephalon by LC-MS

The proteomic profiling, by means of label-free qualitative and quantitative LC-MS analysis of proliferating/undifferentiated vs nonproliferating/differentiated mes-c-myc A1 cell line (A1), has been performed. A1 cells were generated from mouse embryonic central nervous system. The study was aimed at surveying the molecular changes following neural differentiation. The results provide a list of candidate proteins with potential relevance for the transition of A1 cells from the proliferative to the differentiated status. © 2009 American Chemical Society