Strategi Penanggulangan Gangguan Kawasan Konservasi Di Taman Nasional Bunaken (Studi Kasus Di Pulau Mantehage)

This research aims to explore the factors that cause disturbances in conservation areas and determine strategies for dealing with disturbances in Conservation Areas on Mantehage Island, Bunaken National Park, using a combination of qualitative and quantitative methods. The factors causing the disturbance were obtained through observation, questionnaires and in-depth interviews. Furthermore, a SWOT analysis is used to formulate a strategy for overcoming disturbances based on the factors that cause disturbances that have been identified. It was found that the factors causing disturbances in the Bunaken National Park area were: weak law enforcement; lack of trained human resources (Forest Police); limited facilities and supporting infrastructure; and the economic limitations of the local population. Based on the results of the research, the strategy that can be used is the SO Strategy, which uses all the strengths of the organization to take advantage of opportunities. The strategy that can be used is the development of conservation through the protection and enhancement of the conservation of the TNB area by coordinating with communities, groups, institutions and government and developing a conservation partnership program on Mantehage Island through tourism development

Strategi Penanggulangan Gangguan Kawasan Konservasi Di Taman Nasional Bunaken (Studi Kasus Di Pulau Mantehage)

This research aims to explore the factors that cause disturbances in conservation areas and determine strategies for dealing with disturbances in Conservation Areas on Mantehage Island, Bunaken National Park, using a combination of qualitative and quantitative methods. The factors causing the disturbance were obtained through observation, questionnaires and in-depth interviews. Furthermore, a SWOT analysis is used to formulate a strategy for overcoming disturbances based on the factors that cause disturbances that have been identified. It was found that the factors causing disturbances in the Bunaken National Park area were: weak law enforcement; lack of trained human resources (Forest Police); limited facilities and supporting infrastructure; and the economic limitations of the local population. Based on the results of the research, the strategy that can be used is the SO Strategy, which uses all the strengths of the organization to take advantage of opportunities. The strategy that can be used is the development of conservation through the protection and enhancement of the conservation of the TNB area by coordinating with communities, groups, institutions and government and developing a conservation partnership program on Mantehage Island through tourism development

Perbandingan Deteksi Plasmodium Falciparum Dengan Metode Pemeriksaan Mikroskopik Dan Teknik Real-time Polymerase Chain Reaction

: Plasmodium falciparum is one of the species of parasites causing tropical malaria disease. Plasmodium falciparum was reported as often being the major source of pain and even death in most cases. The data released by WHO shows that, globally, 198 millions of malaria cases occurred in 2013 with 548 thousands as cause of death. Microscopic examination is a gold standard for detecting Plasmodium falciparum. Although this method has certain limitations in diagnosing complication infection, phases of parasitemia, and also the capability of laboratory\u27s medical staff factor. Nowadays, there has been innovation in biomolecular department, that is examination using PCR which can accurately detect the plasmodium, due to the DNA amplification. This method however, has not often used by doctors in diagnose malaria disease. The aim of this research is to determine the comparison of malaria detection using microscopic verification of plasmodium falciparum with real-time PCR verification. The method used in this research is diagnostic with 35 blood samples of patient suffering malaria disease. The blood samples from patient\u27s vena were then divided into thick and thin microscopic sample, and some were putted into EDTA tube for DNA extraction in the laboratory using real-time PCR verification. The result of this research shown that sensitivity and specificity rate of PCR is 100% accurate. Conclusion: detection result of plasmodium falciparum using real-time PCR verification produced equal result as microscopic verification

Off-Target Effects of Psychoactive Drugs Revealed by Genome-Wide Assays in Yeast

To better understand off-target effects of widely prescribed psychoactive drugs, we performed a comprehensive series of chemogenomic screens using the budding yeast Saccharomyces cerevisiae as a model system. Because the known human targets of these drugs do not exist in yeast, we could employ the yeast gene deletion collections and parallel fitness profiling to explore potential off-target effects in a genome-wide manner. Among 214 tested, documented psychoactive drugs, we identified 81 compounds that inhibited wild-type yeast growth and were thus selected for genome-wide fitness profiling. Many of these drugs had a propensity to affect multiple cellular functions. The sensitivity profiles of half of the analyzed drugs were enriched for core cellular processes such as secretion, protein folding, RNA processing, and chromatin structure. Interestingly, fluoxetine (Prozac) interfered with establishment of cell polarity, cyproheptadine (Periactin) targeted essential genes with chromatin-remodeling roles, while paroxetine (Paxil) interfered with essential RNA metabolism genes, suggesting potential secondary drug targets. We also found that the more recently developed atypical antipsychotic clozapine (Clozaril) had no fewer off-target effects in yeast than the typical antipsychotics haloperidol (Haldol) and pimozide (Orap). Our results suggest that model organism pharmacogenetic studies provide a rational foundation for understanding the off-target effects of clinically important psychoactive agents and suggest a rational means both for devising compound derivatives with fewer side effects and for tailoring drug treatment to individual patient genotypes