1,954 research outputs found
Do Property Taxes Constitute Personal Liability in Montana?
Do Property Taxes Constitute Personal Liability in Montana
Do Property Taxes Constitute Personal Liability in Montana?
Do Property Taxes Constitute Personal Liability in Montana
Seroprevalence of bluetongue serotype 8 in cattle in the Netherlands in spring 2007, and its consequences
A cross-sectional study was carried out in spring 2007, at the end of the first bluetongue outbreak season, to determine the geographical spread of bluetongue virus serotype 8 (BTV-8) infection in cattle in the Netherlands and the consequences for some production parameters. Blood samples from cattle submitted to the laboratory of the Dutch Animal Health Service for other voluntary and obligatory health programmes were tested serologically for BTV-8. in total, 37,073 samples were tested and 659 (1.78 per cent) were seropositive. The samples came from 5436 herds, of which 45 per cent of herds had only one sample submitted from them. The prevalence was highest in the south of the country, where the outbreak had started, and decreased towards the north. in 340 herds more than 50 per cent of cattle were tested, of which 156 herds were located in infected compartments, and in 37 of these herds (10.9 per cent) at least one positive cow was detected. The average within-herd prevalence in the 37 herds was 39.3 per cent: 2.2 per cent in I I dairy herds, 68.4 per cent in 20 small-scale herds and 14 per cent in four suckler cow herds. The prevalence differed significantly between herd types but did not show a geographical trend. The average net return for milk production amounted to is an element of 2417/cow/year and it decreased significantly on average by is an element of 48/ cow/year in the bluetongue-infected dairy herds during the bluetongue period. on the small-scale farms, the incidence of mortality increased by 3.2 (95 per cent confidence interval [a] 1.2 to 9.1) times in the infected herds during the bluetongue period, but the voluntary culling rate decreased by a factor of 2.3 (95 per cent Cl 1.1 to 4.8)
Axial Flow over a Blunt Circular Cylinder with and without Shear Layer Reattachment
Flow over a circular cylinder with its axis aligned with the free stream was investigated experimentally. Both upstream and downstream faces of the cylinder are sharply truncated. The fineness ratio (length to diameter ratio) was varied and the behavior of the leading-edge separating shear layer and its effect on the wake were studied in water using both flow visualization and PIV techniques. For the moderately large fineness ratio, the shear layer reattaches with subsequent boundary layer growth, whereas over a shorter cylinder the shear layer remains detached. This causes differences in the wake recirculation region and the immediate wake patterns. The shear layer structure was analyzed using the proper orthogonal decomposition (POD). The model in the water channel was sting-mounted and in some cases the effect of model support was detected in the wake measurements. To avoid such disturbance from the model support, an experiment was initiated in air using a magnetic model support and balance system. The drag variation with fineness ratio is presented and discussed in light of the flowfield measurements
FGB1 and WSC3 are in planta-induced beta-glucan-binding fungal lectins with different functions
In the root endophyte Serendipita indica, several lectin-like members of the expanded multigene family of WSC proteins are transcriptionally induced in planta and are potentially involved in beta-glucan remodeling at the fungal cell wall. Using biochemical and cytological approaches we show that one of these lectins, SiWSC3 with three WSC domains, is an integral fungal cell wall component that binds to long-chain beta 1-3-glucan but has no affinity for shorter beta 1-3- or beta 1-6-linked glucose oligomers. Comparative analysis with the previously identified beta-glucan-binding lectin SiFGB1 demonstrated that whereas SiWSC3 does not require beta 1-6-linked glucose for efficient binding to branched beta 1-3-glucan, SiFGB1 does. In contrast to SiFGB1, the multivalent SiWSC3 lectin can efficiently agglutinate fungal cells and is additionally induced during fungus-fungus confrontation, suggesting different functions for these two beta-glucan-binding lectins. Our results highlight the importance of the beta-glucan cell wall component in plant-fungus interactions and the potential of beta-glucan-binding lectins as specific detection tools for fungi in vivo
The Thermopower of Quantum Chaos
The thermovoltage of a chaotic quantum dot is measured using a current
heating technique. The fluctuations in the thermopower as a function of
magnetic field and dot shape display a non-Gaussian distribution, in agreement
with simulations using Random Matrix Theory. We observe no contributions from
weak localization or short trajectories in the thermopower.Comment: 4 pages, 3 figures, corrected: accidently omitted author in the
Authors list, here (not in the article
Structural Features and Domain Organization of Huntingtin Fibrils
Misfolding and aggregation of huntingtin is one of the hallmarks of Huntington disease, but the overall structure of these aggregates and the mechanisms by which huntingtin misfolds remain poorly understood. Here we used site-directed spin labeling and electron paramagnetic resonance (EPR) spectroscopy to study the structural features of huntingtin exon 1 (HDx1) containing 46 glutamine residues in its polyglutamine (polyQ) region. Despite some residual structuring in the N terminus, we find that soluble HDx1 is highly dynamic. Upon aggregation, the polyQ domain becomes strongly immobilized indicating significant tertiary or quaternary packing interactions. Analysis of spin-spin interactions does not show the close contact between same residues that is characteristic of the parallel, in-register structure commonly found in amyloids. Nevertheless, the same residues are still within 20 Ă… of each other, suggesting that polyQ domains from different molecules come into proximity in the fibrils. The N terminus has previously been found to take up a helical structure in fibrils. We find that this domain not only becomes structured, but that it also engages in tertiary or quaternary packing interactions. The existence of spin-spin interactions in this region suggests that such contacts could be made between N-terminal domains from different molecules. In contrast, the C-terminal domain is dynamic, contains polyproline II structure, and lacks pronounced packing interactions. This region must be facing away from the core of the fibrils. Collectively, these data provide new constraints for building structural models of HDx1 fibrils
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