Fingering Instability in a Water-Sand Mixture

The temporal evolution of a water-sand interface driven by gravity is experimentally investigated. By means of a Fourier analysis of the evolving interface the growth rates are determined for the different modes appearing in the developing front. To model the observed behavior we apply the idea of the Rayleigh-Taylor instability for two stratified fluids. Carrying out a linear stability analysis we calculate the growth rates from the corresponding dispersion relations for finite and infinite cell sizes. Based on the theoretical results the viscosity of the suspension is estimated to be approximately 100 times higher than that of pure water, in agreement with other experimental findings.Comment: 11 pages, 12 figures, RevTeX; final versio

Proposal for a Precision Measurement of |Vub|

A new method for a precision measurement of the CKM matrix element |Vub| is discussed, which combines good theoretical control with high efficiency and a powerful discrimination against charm background. The resulting combined theoretical uncertainty on |Vub| is estimated to be 10%.Comment: 4 pages, 2 figures, RevTe

Magnetooptical sum rules close to the Mott transition

We derive new sum rules for the real and imaginary parts of the frequency-dependent Hall constant and Hall conductivity. As an example, we discuss their relevance to the doped Mott insulator that we describe within the dynamical mean-field theory of strongly correlated electron systems.Comment: 4 pages, 4 ps figures; accepted for publication in PR

Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination

The bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. PADs also regulate extracellular vesicle (EV) release, forming a critical part of cellular communication. As PAD-mediated mechanisms in bovine immunology and physiology remain to be investigated, this study profiled deimination signatures in serum and serum-EVs in Bos taurus. Bos EVs were poly-dispersed in a 70−500 nm size range and showed differences in deiminated protein cargo, compared with whole sera. Key immune, metabolic and gene regulatory proteins were identified to be post-translationally deiminated with some overlapping hits in sera and EVs (e.g., immunoglobulins), while some were unique to either serum or serum-EVs (e.g., histones). Protein−protein interaction network analysis of deiminated proteins revealed KEGG pathways common for serum and serum-EVs, including complement and coagulation cascades, viral infection (enveloped viruses), viral myocarditis, bacterial and parasitic infections, autoimmune disease, immunodeficiency intestinal IgA production, B-cell receptor signalling, natural killer cell mediated cytotoxicity, platelet activation and hematopoiesis, alongside metabolic pathways including ferroptosis, vitamin digestion and absorption, cholesterol metabolism and mineral absorption. KEGG pathways specific to EVs related to HIF-1 signalling, oestrogen signalling and biosynthesis of amino acids. KEGG pathways specific for serum only, related to Epstein−Barr virus infection, transcription mis-regulation in cancer, bladder cancer, Rap1 signalling pathway, calcium signalling pathway and ECM-receptor interaction. This indicates differences in physiological and pathological pathways for deiminated proteins in serum-EVs, compared with serum. Our findings may shed light on pathways underlying a number of pathological and anti-pathogenic (viral, bacterial, parasitic) pathways, with putative translatable value to human pathologies, zoonotic diseases and development of therapies for infections, including anti-viral therapies

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the identification of biomarkers for the assessment of their health status is of considerable importance. Peptidylarginine deiminases (PADs) post-translationally convert arginine into citrulline in target proteins in an irreversible manner. PAD-mediated deimination can cause structural and functional changes in target proteins, allowing for protein moonlighting in physiological and pathophysiological processes. PADs furthermore contribute to the release of extracellular vesicles (EVs), which play important roles in cellular communication. In the present study, post-translationally deiminated protein and EV profiles of plasma were assessed in eight seabird species from the Antarctic, representing two avian orders: Procellariiformes (albatrosses and petrels) and Charadriiformes (waders, auks, gulls and skuas). We report some differences between the species assessed, with the narrowest EV profiles of 50−200 nm in the northern giant petrel Macronectes halli, and the highest abundance of larger 250−500 nm EVs in the brown skua Stercorarius antarcticus. The seabird EVs were positive for phylogenetically conserved EV markers and showed characteristic EV morphology. Post-translational deimination was identified in a range of key plasma proteins critical for immune response and metabolic pathways in three of the bird species under study; the wandering albatross Diomedea exulans, south polar skua Stercorarius maccormicki and northern giant petrel. Some differences in Gene Ontology (GO) biological and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for deiminated proteins were observed between these three species. This indicates that target proteins for deimination may differ, potentially contributing to a range of physiological functions relating to metabolism and immune response, as well as to key defence mechanisms. PAD protein homologues were identified in the seabird plasma by Western blotting via cross-reaction with human PAD antibodies, at an expected 75 kDa size. This is the first study to profile EVs and to identify deiminated proteins as putative novel plasma biomarkers in Antarctic seabirds. These biomarkers may be further refined to become useful indicators of physiological and immunological status in seabirds—many of which are globally threatened

Bleaching impacts on carbonate production in the Chagos Archipelago: influence of functional coral groups on carbonate budget trajectories

This is the final version. Available on open access from Springer Verlag via the DOI in this recordReefs in the remote Chagos Archipelago (central Indian Ocean) were severely affected by sea-surface temperature warming and coral bleaching in 2015-2016. Here we assess the impacts of this event on community composition and reef carbonate production at twelve fore reefs sites across three atolls. Bleaching caused a 69% decline in coral cover, mostly driven by mortality of tabular Acropora spp., and a 77% decline in mean coral carbonate production (2015: 13.1±4.8; 2018: 3.0±1.2 kg CaCO3 m2 yr-1). Changes were accompanied by a major shift from competitive to stress-tolerant coral taxa, with magnitudes of decline comparable to those reported elsewhere in the Indian Ocean, despite inter-site differences in dominant coral species. These trends differ from those on reefs dominated by stress-tolerant taxa, which experienced minor declines in production post-warming. The study highlights the potential for different suites of functional coral groups to drive divergent post-bleaching budget responses.Bertarelli Foundatio

Magnetic Soret effect: Application of the ferrofluid dynamics theory

The ferrofluid dynamics theory is applied to thermodiffusive problems in magnetic fluids in the presence of magnetic fields. The analytical form for the magnetic part of the chemical potential and the most general expression of the mass flux are given. By employing these results to experiments, global Soret coefficients in agreement with measurements are determined. Also an estimate for a hitherto unknown transport coefficient is made.Comment: 7 pages, 2 figure