10,249 research outputs found
Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana
We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is within 1.9 centimorgans, or approx 270 kilobase pairs, of the mapped DNA fragments. The map was based on the meiotic segregation of markers in two different crosses. The restriction fragment length polymorphism linkage groups were integrated with the five classically mapped linkage groups by virtue of mapped mutations included in these crosses. Markers consist of both cloned Arabidopsis genes and random low-copy-number genomic DNA clones that are able to detect polymorphisms with the restriction enzymes EcoRI, Bgl II, and/or Xba I. These cloned markers can serve as starting points for chromosome walking, allowing for the isolation of Arabidopsis genes of known map location. The restriction fragment length polymorphism map also can associate clones of unknown gene function with mutant phenotypes, and vice versa
5d-5f Electric-multipole Transitions in Uranium Dioxide Probed by Non-resonant Inelastic X-ray Scattering
Non-resonant inelastic x ray scattering (NIXS) experiments have been
performed to probe the 5d-5f electronic transitions at the uranium O(4,5)
absorption edges in uranium dioxide. For small values of the scattering vector
q, the spectra are dominated by dipole-allowed transitions encapsulated within
the giant resonance, whereas for higher values of q the multipolar transitions
of rank 3 and 5 give rise to strong and well-defined multiplet structure in the
pre-edge region. The origin of the observed non-dipole multiplet structures is
explained on the basis of many-electron atomic spectral calculations. The
results obtained demonstrate the high potential of NIXS as a bulk-sensitive
technique for the characterization of the electronic properties of actinide
materials.Comment: Submitted to Physical Review Letters on 31 December 200
Tagged particle in a sheared suspension: effective temperature determines density distribution in a slowly varying external potential beyond linear response
We consider a sheared colloidal suspension under the influence of an external
potential that varies slowly in space in the plane perpendicular to the flow
and acts on one selected (tagged) particle of the suspension. Using a
Chapman-Enskog type expansion we derive a steady state equation for the tagged
particle density distribution. We show that for potentials varying along one
direction only, the tagged particle distribution is the same as the equilibrium
distribution with the temperature equal to the effective temperature obtained
from the violation of the Einstein relation between the self-diffusion and
tagged particle mobility coefficients. We thus prove the usefulness of this
effective temperature for the description of the tagged particle behavior
beyond the realm of linear response. We illustrate our theoretical predictions
with Brownian dynamics computer simulations.Comment: Accepted for publication in Europhys. Let
Well-posedness and asymptotic behavior of a multidimensional model of morphogen transport
Morphogen transport is a biological process, occurring in the tissue of
living organisms, which is a determining step in cell differentiation. We
present rigorous analysis of a simple model of this process, which is a system
coupling parabolic PDE with ODE. We prove existence and uniqueness of solutions
for both stationary and evolution problems. Moreover we show that the solution
converges exponentially to the equilibrium in topology. We
prove all results for arbitrary dimension of the domain. Our results improve
significantly previously known results for the same model in the case of one
dimensional domain
Robust formation of morphogen gradients
We discuss the formation of graded morphogen profiles in a cell layer by
nonlinear transport phenomena, important for patterning developing organisms.
We focus on a process termed transcytosis, where morphogen transport results
from binding of ligands to receptors on the cell surface, incorporation into
the cell and subsequent externalization. Starting from a microscopic model, we
derive effective transport equations. We show that, in contrast to morphogen
transport by extracellular diffusion, transcytosis leads to robust ligand
profiles which are insensitive to the rate of ligand production
Electronic structure of the muonium center as a shallow donor in ZnO
The electronic structure and the location of muonium centers (Mu) in
single-crystalline ZnO were determined for the first time. Two species of Mu
centers with extremely small hyperfine parameters have been observed below 40
K. Both Mu centers have an axial-symmetric hyperfine structure along with a
[0001] axis, indicating that they are located at the AB_{O,//} and BC_{//}
sites. It is inferred from their small ionization energy (~6 meV and 50 meV)
and hyperfine parameters (~10^{-4} times the vacuum value) that these centers
behave as shallow donors, strongly suggesting that hydrogen is one of the
primary origins of n type conductivity in as-grown ZnO.Comment: 4 pages, 4 figures, submitted to PR
Development of Readout Interconnections for the Si-W Calorimeter of SiD
The SiD collaboration is developing a Si-W sampling electromagnetic
calorimeter, with anticipated application for the International Linear
Collider. Assembling the modules for such a detector will involve special
bonding technologies for the interconnections, especially for attaching a
silicon detector wafer to a flex cable readout bus. We review the interconnect
technologies involved, including oxidation removal processes, pad surface
preparation, solder ball selection and placement, and bond quality assurance.
Our results show that solder ball bonding is a promising technique for the Si-W
ECAL, and unresolved issues are being addressed.Comment: 8 pages + title, 6 figure
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