7 research outputs found
Karakterizacija Ī²-glukana izoliranih iz pivskoga kvasca te osuÅ”enih razliÄitim metodama
Two different procedures have been used for isolation of water-insoluble Ć-glucans from brewerās yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained Ć-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. Ć-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of Ć-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1ā3)-Ć-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in Ć-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates.Netopljivi Ī²-glukani izolirani su iz pivskoga kvasca primjenom dvaju razliÄitih postupaka: alkalno-kiselinskog (AK) i alkalno-kiselinskog s uklanjanjem manoproteina (AKM). Dobiveni pripravci Ī²-glukana osuÅ”eni su na zraku, liofilizacijom i rasprÅ”ivanjem uz prethodnu obradu ultrazvukom. Takvi su pripravci, dobiveni AK i AKM postupcima, imali približno iste vrijednosti suhe tvari, ukupnih polisaharida i proteina, te udjele ugljika, duÅ”ika i kisika odreÄene organskom mikroanalizom. Na udio Ī²-glukana u ukupnim polisaharidima bitno su utjecali razliÄiti postupci izolacije. FT-IR spektri svih pripravaka imali su izgled tipiÄan za (1ā3)-Ī²-D-glukan. SuÅ”enjem na zraku i liofilizacijom doÅ”lo je do znaÄajnih promjena strukture Äestica i aglomeracije Ć-glukana. SuÅ”enje rasprÅ”ivanjem uz prethodnu obradu ultrazvukom nije dovelo do stvaranja nakupina Äestica kao ni do znatnih promjena njihove strukture
Karakterizacija Ī²-glukana izoliranih iz pivskoga kvasca te osuÅ”enih razliÄitim metodama
Two different procedures have been used for isolation of water-insoluble Ć-glucans from brewerās yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained Ć-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. Ć-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of Ć-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1ā3)-Ć-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in Ć-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates.Netopljivi Ī²-glukani izolirani su iz pivskoga kvasca primjenom dvaju razliÄitih postupaka: alkalno-kiselinskog (AK) i alkalno-kiselinskog s uklanjanjem manoproteina (AKM). Dobiveni pripravci Ī²-glukana osuÅ”eni su na zraku, liofilizacijom i rasprÅ”ivanjem uz prethodnu obradu ultrazvukom. Takvi su pripravci, dobiveni AK i AKM postupcima, imali približno iste vrijednosti suhe tvari, ukupnih polisaharida i proteina, te udjele ugljika, duÅ”ika i kisika odreÄene organskom mikroanalizom. Na udio Ī²-glukana u ukupnim polisaharidima bitno su utjecali razliÄiti postupci izolacije. FT-IR spektri svih pripravaka imali su izgled tipiÄan za (1ā3)-Ī²-D-glukan. SuÅ”enjem na zraku i liofilizacijom doÅ”lo je do znaÄajnih promjena strukture Äestica i aglomeracije Ć-glukana. SuÅ”enje rasprÅ”ivanjem uz prethodnu obradu ultrazvukom nije dovelo do stvaranja nakupina Äestica kao ni do znatnih promjena njihove strukture
Characterization of b-glucans isolated from brewerās yeast and dried by different methods
Two different procedures have been used for isolation of water-insoluble b-glucans from brewerās yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained b-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. b-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of b-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1Ā®3)-b-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in b-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates
Toksikokinetika prometrina u mozgu miŔeva
Prometryne is a methylthio-s-triazine herbicide. Signifi cant trace amounts are found in the environment,
mainly in water, soil, and food plants. The aim of this study was to establish brain and blood prometryne levels after single oral dose (1 g kg-1) in adult male and female mice. Prometryne was measured using the GC/MS assay at 1, 2, 4, 8, and 24 h after prometryne administration. Peak brain and blood prometryne values were observed 1 h after administration and they decreased in a time-dependent manner. Male mice had consistently higher brain and blood prometryne levels than female mice. The observed prometryne
kinetics was similar to that reported for the structurally related herbicide atrazine.Prometrin je metiltio-s-triazinski herbicid. ZnaÄajne koliÄine prometrina zaostaju u tragovima u okoliÅ”u, poglavito u vodi, tlu i biljkama koje rabimo za prehranu. Cilj je rada izmjeriti koliÄinu prometrina koja se apsorbira u mozgu i krvi nakon primijenjene akutne oralne doze (1 g kg-1 tjelesne mase) u odraslih miÅ”eva
obaju spolova. Razine prometrina u mozgu i krvi izmjerene su GC/MS-om tijekom 1., 2., 4., 8. i 24. sata nakon izlaganja. UtvrÄeno je da je udio prometrina koji se zadržava u živÄanom tkivu relativno nizak ali detektabilan u odnosu na koncentraciju u krvi i koncentraciju primijenjene doze. NajviÅ”e koncentracije u krvi i maseni udjeli u mozgu zabilježeni su tijekom 1. sata nakon izlaganja, a s vremenom izmjerene vrijednosti znaÄajno opadaju. UoÄena je znaÄajna razlika izmeÄu mužjaka i ženki pri Äemu mužjaci imaju
znaÄajno viÅ”e razine prometrina u mozgu i krvi nego ženke. Opisana toksikokinetika prometrina pokazuje sliÄnosti s otprije opisanom i poznatom toksikokinetikom strukturalno sliÄnog herbicida atrazina
Characterization of Ć-Glucans Isolated from Brewerās Yeast and Dried by Different Methods
Two different procedures have been used for isolation of water-insoluble Ć-glucans from brewerās yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained Ć-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. Ć-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of Ć-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1ā3)-Ć-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in Ć-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates
The effects of prometryne on subchronically treated mice evaluated by SCGE assay
Prometryne is a methylthio-s-triazine herbicide used to control annual broadleaf and grass weeds in many cultivated plants. Significant traces are documented in environment, mainly water, soil and plants used for human and domestic animal nutrition. Data on the toxic effects of prometryne and other methylthios-triazine have scorcely been published. The goal of this study was to investigate if prometryne, applied orally, could induce DNA damage in mouse leukocytes, in subchronical
in vivo
experimental design. Three different doses of prometryne were applied
per os
repeatedly every 48 hours. After the 7th dose (day 14) and the 14th dose (day 28) blood leucocytes were analyzed by alkaline Single Cell Gel Electrophoresis (Comet) assay. The results of three different comet parameters showed general increase in Olive tail moment, tail length and tail intensity values in treated groups of animals. The increase in measured values was almost proportional to the dose received and the time of exposure. We conclude that prometryne or its metabolic residues have the potential to induce processes that cause genotoxic effects on leukocytes on mice in
in vivo
repeated exposure