1,379 research outputs found
Human HT-29 colon carcinoma cells contain mucarinic M receptors coupled to phosphoinositide metabolism
Five different musearlnie receptor subtypes ean be distinguished by the differenees in their amino aeid sequence, the eoupled signal transduetion system, pharmaeologieal binding properties and aetivation of ionie fluxes. The present study served to eharaeterize the binding profile of musearlnie receptors in human eolon eareinoma eells (HT-29) using seleetive musearlnie antagonists. The affinities of the compounds were eompared with their poteney to inhibit cholinergieally-aetivated phosphoinositide metabolism. Pirenzepine displaced [H]N-methyl-scopolamine binding and inhibited inositolphosphate (IP) release with potencies typieal of those of non-M receptors. The M subtype-selective antagonists sila-hexocyelium and hexahydro-sila-difenidol bad high affinity to the musearlnie reeeptors in HT-29 cells (K0 = 3.1 nM and 27 nM, respectively) and inhibited IP release at nanomolar concentrations. The M receptor antagonists, AF-DX 116 and methoctramine, had low antimusearinic poteneies. Our results demonstrate that HT-29 human colon earcinoma cells contain an apparently pure population of M receptors. These cells could serve as a model system for further investigations coneerning regulatory and signal transduction mechanisms associated with glandular muscarinic M receptors
Syntbesis and Properries of the Selective Antimuscarinic Agent Cyclohexylphenyl(3-piperidinopropyl)silanol
Die Synthese des selektiven Antimuskarinikums Cyclohexylpheny\{3-piperidinopropyl)sila· nol (1 b) wird beschrieben. 1 b wurde - ausgehend von (3·Chlorpropyl)trimethoxysilan - durch eine vierstufige Reaktionsfolge erhalten und als Hydrochlorid 2b mit einer Gesamtausbeute von etwa 45°/o isoliert. - 1 b ist aufgrund seiner großen pharmakologischen Se· lektivität zu einer Standardsubstanz in der experimentellen Pharmakologie bei der Differenzierung von Muskarinrezeptoren geworden.The synthesis of thc selective antimuscarinic agent cyclohexylphenyl(3-piperidinopropyl)silanol (1 b) is described. Starting with (3-chloropropyl)trimethoxysilane, I b was obtained by four reaction steps and isolated as hydrochloride 2b with a total yield of about 45°/o. - Because of its high pharmacological selectivity 1 b has become a reference drug in experimental pharmacology for the differentiation of muscarinic rcceptors
The Magnetic Casimir Effect
The Casimir effect results from alterations of the zero-point electromagnetic
energy introduced by boundary-conditions. For ferromagnetic layers separated by
vacuum (or a dielectric) such boundary-conditions are influenced by the
magneto-optical Kerr effect. We will show that this gives rise to a long-range
magnetic interaction and discuss the effect for two different configurations
(magnetization parallel and perpendicular to the layers). Analytical
expressions are derived for two models and compared to numerical calculations.
Numerical calculations of the effect for Fe are also presented and the
possibility of an experimental observation of the Casimir magnetic interaction
is discussed
Temperature dependence of the Casimir effect between metallic mirrors
We calculate the Casimir force and free energy for plane metallic mirrors at
non-zero temperature. Numerical evaluations are given with temperature and
conductivity effects treated simultaneously. The results are compared with the
approximation where both effects are treated independently and the corrections
simply multiplied. The deviation between the exact and approximated results
takes the form of a temperature dependent function for which an analytical
expression is given. The knowledge of this function allows simple and accurate
estimations at the % level.Comment: 8 pages, 4 figures, uses RevTe
The Casimir force and the quantum theory of lossy optical cavities
We present a new derivation of the Casimir force between two parallel plane
mirrors at zero temperature. The two mirrors and the cavity they enclose are
treated as quantum optical networks. They are in general lossy and
characterized by frequency dependent reflection amplitudes. The additional
fluctuations accompanying losses are deduced from expressions of the optical
theorem. A general proof is given for the theorem relating the spectral density
inside the cavity to the reflection amplitudes seen by the inner fields. This
density determines the vacuum radiation pressure and, therefore, the Casimir
force. The force is obtained as an integral over the real frequencies,
including the contribution of evanescent waves besides that of ordinary waves,
and, then, as an integral over imaginary frequencies. The demonstration relies
only on general properties obeyed by real mirrors which also enforce general
constraints for the variation of the Casimir force.Comment: 18 pages, 6 figures, minor amendment
Dynamical Casimir effect at finite temperature
Thermal effects on the creation of particles under the influence of
time-dependent boundary conditions are investigated. The dominant temperature
correction to the energy radiated by a moving mirror is derived by means of
response theory. For a resonantly vibrating cavity the thermal effect on the
number of created photons is obtained non-perturbatively. Finite temperatures
can enhance the pure vacuum effect by several orders of magnitude. The
relevance of finite temperature effects for the experimental verification of
the dynamical Casimir effect is addressed.Comment: 9 LaTex page
The Casimir Problem of Spherical Dielectrics: Numerical Evaluation for General Permittivities
The Casimir mutual free energy F for a system of two dielectric concentric
nonmagnetic spherical bodies is calculated, at arbitrary temperatures. The
present paper is a continuation of an earlier investigation [Phys. Rev. E {\bf
63}, 051101 (2001)], in which F was evaluated in full only for the case of
ideal metals (refractive index n=infinity). Here, analogous results are
presented for dielectrics, for some chosen values of n. Our basic calculational
method stems from quantum statistical mechanics. The Debye expansions for the
Riccati-Bessel functions when carried out to a high order are found to be very
useful in practice (thereby overflow/underflow problems are easily avoided),
and also to give accurate results even for the lowest values of l down to l=1.
Another virtue of the Debye expansions is that the limiting case of metals
becomes quite amenable to an analytical treatment in spherical geometry. We
first discuss the zero-frequency TE mode problem from a mathematical viewpoint
and then, as a physical input, invoke the actual dispersion relations. The
result of our analysis, based upon the adoption of the Drude dispersion
relation at low frequencies, is that the zero-frequency TE mode does not
contribute for a real metal. Accordingly, F turns out in this case to be only
one half of the conventional value at high temperatures. The applicability of
the Drude model in this context has however been questioned recently, and we do
not aim at a complete discussion of this issue here. Existing experiments are
low-temperature experiments, and are so far not accurate enough to distinguish
between the different predictions. We also calculate explicitly the
contribution from the zero-frequency mode for a dielectric. For a dielectric,
this zero-frequency problem is absent.Comment: 23 pages, LaTeX, 7 ps figures; expanded discussion, especially in
Sec. 5. To appear in Phys. Rev.
Resonant photon creation in a three dimensional oscillating cavity
We analyze the problem of photon creation inside a perfectly conducting,
rectangular, three dimensional cavity with one oscillating wall. For some
particular values of the frequency of the oscillations the system is resonant.
We solve the field equation using multiple scale analysis and show that the
total number of photons inside the cavity grows exponentially in time. This is
also the case for slightly off-resonance situations. Although the spectrum of a
cavity is in general non equidistant, we show that the modes of the
electromagnetic field can be coupled, and that the rate of photon creation
strongly depends on this coupling. We also analyze the thermal enhancement of
the photon creation.Comment: 13 pages. New section on off-resonance motion is included. To appear
in Physical Review
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