10 research outputs found
Effect of benzimidazole fungicides and calcium chloride on Alternaria alternata and Penicillium expansum rot during storage of pears
The present study assayed the effect of the fungicides benomyl, methyl-thiophanate, thiabendazole and calcium chloride on fungal decay of pears caused by Alternaria alternata and Penicillium expansum. Both in vitro and in vivo, the efficacy of fungicides alone against the two fungi was shownto be weak and without any practical interest. In vitro, CaCl2 alone was tolerated by both species at more than 4%. In vivo and at low temperature, CaCl2 significantly reduced fungal decay when used at 4 and 6%. The association fungicides - CaCl2 (4%) allowed a better control of A. alternata
Callus Induction from Carob (Ceratonia siliqua L.) Seedlings and Leaves of Mature Tree
Callus induction was successfully carried out from several explants of carob (Ceratonia siliqua L.). Callogenesis from the apex was tested on three different media containing Woody Plant Medium (WPM), Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) macronutrients supplemented with two different hormonal solutions: benzylaminopurine (BAP) at 4.44 ”M alone, or 2.22 ”M of BAP plus 5 ”M of 2-naphthalineacetic acid (NAA). Primary callus formation was obtained on a medium containing 88% WPM macronutrients. Callus formation from other parts of the plant was as follows:
â Cotyledon embryos extracted from immature seeds (85% success rate on WPM medium, containing 4.44 ”M BAP and 5 ”M NAA);
â Cotyledon leaves taken from 7-day-old seedlings, obtained from in vitro germination of seeds (62% success rate on WPM medium, containing 4.44 ”M BAP and 5 ”M NAA);
â Hypocotyls taken from 7-day-old seedlings (55% success rate on WPM containing 2.22 ”M BAP and 5 ”M NAA);
â Differentiated leaves taken from mature tree (84% success rate on WPM medium, containing 4.44 ”M of BA and 2.26 ”M of NAA).
In general, production of primary calli and their growth after transplantation was better on WPM medium supplemented with 2.5 ”M NAA and 2.22 ”M BAP
Micropropagation of Carob tree (Ceratonia siliqua L.) by cotyledonary buds
La micropropagation du Caroubier (Ceratonia siliqua L.) par culture de bourgeons cotylédonaires de plantules
ùgées de 13 jours a été étudiée sur le milieu WPM additionné de microéléments et de vitamines MS. La
comparaison de lâeffet de quatre cytokinines (BAP, zĂ©atine, kinĂ©tine et 2-iP) a montrĂ© que la croissance des
pousses est surtout stimulĂ©e par la zĂ©atine (1 mg/l) ; alors que lâorganogenĂšse se fait mieux en prĂ©sence de la BAP
ou de la zĂ©atine (1 mg/l). Lâeffet de quatre auxines (AIA, AIB, ANA et 2,4-D) Ă faible concentration (0,1 mg/l)
combinĂ©es Ă la BAP (0,5 mg/l) a Ă©tĂ© aussi testĂ©, et a montrĂ© que lâANA favorise la croissance des plantules et la
nĂ©oformation de tiges. Toutefois, lâassociation de lâAG3 Ă diffĂ©rentes concentrations avec la BAP (0,5 mg/l) nâa
pas eu dâeffet significatif sur la culture de ces explants. La multiplication des pousses a Ă©tĂ© favorisĂ©e par des
concentrations moyennes de BAP (0,5 Ă 1 mg/l) seule ou associĂ©e avec lâAG3 (0,2 mg/l). Parmi les auxines
testĂ©es au cours de la phase dâenracinement, lâAIB (2 mg/l) sâest avĂ©rĂ© la plus efficace pour la rhizogenĂšse.In vitro micropropagation of Carob tree (Ceratonia siliqua L.) by cotyledonary buds taken from 13 day old
seedlings was attempted on Woody Plant Medium (WPM) supplemented with microelements and Murashige and
Skoog (MS) vitamins. Comparison of the effect of four cytokinines (benzylaminopurine, zeatin, kinetinand 2-iP)
has shown that seedling growth was stimulated by zeatin at 1 mg/L, while organogenesis was better under BAP or
zeatin at 1 mg/L. The effect of four auxins (AIA, AIB, ANA and 2,4-D) at low concentrations (0.1 mg/L)
combined with BAP at 0.5 mg/L has shown that Naphthalene Acetic Acid (NAA) stimulates better stem
formation and growth. Moreover, combination of Gibberellic Acid (GA3) at different concentrations with BAP at
0.5 mg/L did not show a significant effect on growth. Shoot multiplication was stimulated by medium
concentrations of BAP (0.5 - 1 mg/L) alone or with GA3 at 0.2 mg/L. Among the auxins tested during rooting,
Indol-3-Butyric Acid (IBA) (2 mg/L) was found to be the most efficient for root formation
Callus Induction from Carob (Ceratonia siliqua L.) Seedlings and Leaves of Mature Tree
Callus induction was successfully carried out from several explants of carob (Ceratonia siliqua L.). Callogenesis from the apex was tested on three different media containing Woody Plant Medium (WPM), Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) macronutrients supplemented with two different hormonal solutions: benzylaminopurine (BAP) at 4.44 ”M alone, or 2.22 ”M of BAP plus 5 ”M of 2-naphthalineacetic acid (NAA). Primary callus formation was obtained on a medium containing 88% WPM macronutrients. Callus formation from other parts of the plant was as follows:
â Cotyledon embryos extracted from immature seeds (85% success rate on WPM medium, containing 4.44 ”M BAP and 5 ”M NAA);
â Cotyledon leaves taken from 7-day-old seedlings, obtained from in vitro germination of seeds (62% success rate on WPM medium, containing 4.44 ”M BAP and 5 ”M NAA);
â Hypocotyls taken from 7-day-old seedlings (55% success rate on WPM containing 2.22 ”M BAP and 5 ”M NAA);
â Differentiated leaves taken from mature tree (84% success rate on WPM medium, containing 4.44 ”M of BA and 2.26 ”M of NAA).
In general, production of primary calli and their growth after transplantation was better on WPM medium supplemented with 2.5 ”M NAA and 2.22 ”M BAP
Seedling growth of Rhamnus alaternus and Rhamnus ludovici-salvatoris under Mediterranean conditions
PĂłster presentado en el XIX ReuniĂłn de la Sociedad Española de FisiologĂa Vegetal (SEFV) - XII Congreso Hispano-Luso de FisiologĂa Vegetal, celebrado del 21 al 24 de junio de 2011 en CastellĂłn de la Plana (España)Peer Reviewe
Primary malignant melanoma of prostate
Primary genitourinary melanoma accounts for less than one per cent of all cases of melanoma. Most cases attributed to the prostate actually originate from the prostatic urethra. Due to its infrequency, primary malignant melanoma of the genitourinary tract presents a difficult diagnostic and management challenge. We report a case of primary malignant melanoma of the prostate found during transurethral resection of the prostate