Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions

The chemical composition of the essential oils of Seseli gracile Waldst. & Kit. from natural habitat (Derdap Gorge, Serbia) and from cultivated plants (Belgrade, Serbia) were characterized. The essential oils of the root, aerial parts, inflorescence and fruit were analyzed by GC/MS and GC/FID. Monoterpene hydrocarbons were the main compounds in the essential oil of aerial parts (45.2-93.0 %), inflorescences (84.1 and 90.0 %) and fruit (85.0 %). Polyacetylenes (38.8 and 87.6 %) were dominant in the essential oil of root. The cluster analysis revealed that there were significant differences in the chemical composition of the S. gracile oils at different phenological stages. On the other hand, essential oils from the aerial parts from natural and cultivated plants showed quite uniform qualitative composition. The aerial parts essential oil from natural habitat contained higher content of para-cymene (mean values 17.3 vs. 6.5 %) and lower amounts of terpinolene (mean values 23.1 vs. 49.9 %). Also polyacetylene falcarinol was present only in the aerial parts samples from natural habitat. The essential oil of inflorescences from natural habitat contained higher concentration of terpinolene, quite similar amount of para-cymene and lower content of a-pinene

Stipetum novakii ass. nova – a new association of serpentine rocky grassland vegetation (Halacsyetalia sendtneri) in Serbia

Phytosociological characteristics of grassland communities above serpentines (order Halacsyetalia sendtneri H. Ritter-Studni~ka 1970) in Serbia, are analyzed according to Braun-Blanquet methodology. In order to detect the basic floristic differentiation of analyzed communities ordinary correspondence analysiswas applied. Cluster analysis was also performed to see the structure and separation of the communities based on the floristic composition. In order to determine diagnostic species, fidelity indices with presence/ absence data and the size of all groups standardized to equal size were calculated. The new association Stipetum novakii is described in open rocky serpentine grasslands in Brdjani Gorge

Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions

The chemical composition of the essential oils of Seseli gracile Waldst. & Kit. from natural habitat (Đerdap Gorge, Serbia) and from cultivated plants (Belgrade, Serbia) were characterized. The essential oils of the root, aerial parts, inflorescence and fruit were analyzed by GC/MS and GC/FID. Monoterpene hydrocarbons were the main compounds in the essential oil of aerial parts (45.2–93.0 %), inflorescences (84.1 and 90.0 %) and fruit (85.0 %). Polyacetylenes (38.8 and 87.6 %) were dominant in the essential oil of root. The cluster analysis revealed that there were significant differences in the chemical composition of the S. gracile oils at different phenological stages. On the other hand, essential oils from the aerial parts from natural and cultivated plants showed quite uniform qualitative composition. The aerial parts essential oil from natural habitat contained higher content of para-cymene (mean values 17.3 vs. 6.5 %) and lower amounts of terpinolene (mean values 23.1 vs. 49.9 %). Also polyacetylene falcarinol was present only in the aerial parts samples from natural habitat. The essential oil of inflorescences from natural habitat contained higher concentration of terpinolene, quite similar amount of para- -cymene and lower content of α-pinene. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173021 Grant no. 173030

The digital database of aquatic and semiaquatic vegetation in Serbia

Despite the absence of a centralized national phytocoenological database of Serbia, over 16,000 relevés of all vegetation types have been gathered and stored in the database “Phytocoenosis of Serbia” as a result of the Project “Habitats of Serbia”. However, as the data on aquatic and semiaquatic vegetation comprise only 5% of the collected relevés, the aim of this paper is to expand on this, complete the database and present the data on these vegetation types in Serbia. The work included the expansion of the existing database with the addition of relevant sources, their digitalization, using Flora and Turboveg programs, and their subsequent georeferencing, using OziExplorer and DIVAGIS softwares. Consequently, the phytocoenological database on aquatic and semiaquatic vegetation in Serbia now stores 1,720 relevés from 243 phytocoenological tables, collected and published by 24 authors during the period of 70 years (1940-2010), with the majority of the relevés collected over the last decade (56.40%). Phragmito-Magno-Caricetea Klika in Klika et Novák 1941 (Syn: Phragmitetea communis R. Tx. et Prsg. 1942), Potametea Klika in Klika et Novák 1941 (Syn: Potametea R. Tx. et Preising 1942) and Lemnetea de Bolós et Masclans 1955 (Syn: Lemnetea minoris W. Koch et R. Tx. 1955) have proven to be the most represented classes, while Scirpo-Phragmitetum W. Koch 1926 (nomen ambiguum) and Salvinio natantis-Spirodeletum polyrrhizae Slavnić 1956 are associations with the highest number of relevés in the database. Accordingly, the most common species (>500 relevés) are Ceratophyllum demersum L. subsp. demersum, Lemna minor L. and Spirodela polyrhiza (L.) Schleiden

An overview of aquatic vegetation in Serbia

The majority of aquatic vegetation studies in the Middle Danube Basin (Serbia) were done independently during the last two decades, including data from small areas, which resulted in several classification solutions. The main purpose of this paper was to develop a numerical classification of the aquatic vegetation in Serbia, without fitting the vegetation groups into the existing phytocoenological classification scheme. Datasets of (i) surface and subsurface vegetation of free-floating duckweeds, ferns, liverworts and bladderworts, and (ii) vegetation of free-floating hydrocharids, submerged occasionally anchored ceratophyllids and rooted aquatic vegetation were compiled from phytocoenological releves (974). In order to fill the geographical and methodological gaps of these datasets, additional data matrix (iii) was compiled from sample quadrats (1055), collected during the aquatic vegetation season (summer months) of 2009, 2010 and 2011 at 31 lakes in Serbia. The datasets were analyzed using SYN-TAX 5.1 program, by non-metric hierarchical clustering OrdClAn and the Goodman-Kruskal's gamma resemblance coefficient. The cluster analysis revealed 28 aquatic vegetation groups (VG), of which three have been recognized as new vegetation units for the area of study: VG dominated by Vallisneria spiralis and Potamogeton perfoliatus, VG characterized by Polygonum amphibium and VG with Paspalum paspaloides as a constant. Geographical ranges and constant, diagnostic and dominant species of vegetation groups were determined

Campanula cichoracea

The taxonomically intricate Campanula lingulata complex confined to the Balkan Peninsula is reviewed using molecular and morphological data. An extensive sample of 62 individuals for phylogenetic analyses and 402 individuals for morphometric analysis from 17 populations across the species range was used. The phylogenetic analyses based on two chloroplast intergenic spacers (trnG(UCC)-trnS(GCU) and psbA-trnH) and morphological analysis based on 50 characters revealed two allopatrically distributed lineages of the C. lingulata complex that comprise individuals from the C and S Balkans, respectively. Both molecular and morphological data allowed us to re-establish C. cichoracea Sm., a species endemic to Thessaly in Greece. This species can easily be distinguished from C. lingulata s.str. by its calyx appendages hairy on the margins and adaxial side, and ovary continuously downwards hairy all over the surface. Molecular characters that can be used to distinguish these two species comprise four parsimony-informative substitutions within trnG(UCC)-trnS(GCU), and a microsatellite with a dinucleotide (AT) motif present only within the psbA-trnH region in C. lingulata. Further studies are required for resolving the taxonomic status of the remaining Macedonian and Rhodopean sub-lineages from the S Balkans