Excretion of lysine by Micrococcus glutamicus

Analysis of intracellular and extracellular lysine concentration during lysine fermentation by Micrococcus glutamicus AEC RN-13-6/1 indicated that lysine excretion occurs against a concentration gradient towards the end of the fermentation period. The capacity to excrete lysine against a concentration gradient may be a factor contributing to the high yield of lysine

Purification and properties of diaminopimelate decarboxylase of Micrococcus glutamicus

Diaminopimelate decarboxylase (EC 4.1.1.20) of Micrococcus glutamicus ATCC 13059 was purified to homogeneity. The enzyme had an apparent molecular weight of 191,000 as determined by gel filtration on Sephadex G-200. At protein concentrations of 20 and 10 μg per ml and in the absence of pyridoxal-5'-phosphate, it dissociated into a species of molecular weight 94,000. The polypeptide chain molecular weight as determined by sodium dodecyl sulphate Polyacrylamide gel electrophoresis was 100,000. The Km formeso diaminopimelate was 0.5 mM and that for pyridoxal-5'-phosphate was 0.6 μM. Sulphydryl groups and pyridoxal-5'-phosphate were essential for activity and stability. The enzyme was inhibited significantly by L-lysine and DL-aspartic β-semialdehyde

Synthesis and characterization of acrylamide substituted sulfanilamide based calamitic LCs: effect of terminal group on phase behavior

In the present study, we have prepared two phenyl ring substituted sulfanilamide bearing acrylamide linking groups. The four derivatives (C1-C4) were prepared by changing the left n-alkoxy terminal group. The liquid crystalline properties of the synthesized sulfanilamide-based compounds were investigated using POM and DSC studies. All four materials exhibit nematic liquid crystalline properties during both heating and cooling. They also demonstrate a broad temperature range and thermal stability. Further, we have studied the structure-property relationship to gain a better understanding of the effect of the linking group and variable alkoxy tail group on mesomorphic behavior. The results indicate that thermal stability, mesophase temperature range, and liquid crystalline properties depend on the linking group and variable alkyl chain length

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Not AvailableEight CMS lines and seven restorers were crossed in a line x tester fashion to elucidate the information on combining ability for achene yield, yield components and oil content and also to know the nature of gene action involved in inheritance ofimportant traits. A total of 56 crosses were evaluated for 9 different quantitative traits. The analysis of variance revealed the existence of a statistically significant difference between the genotypes used in crossing, which confirmed the differences among selected parents. Among the lines, CMS-853A and CMS-852A exhibited significant gca effect for most ofthe characters studied except autogamy (%) and were found to be the best combiners. Likewise, tester EC-601878 was the best combiner for plant height, head diameter, days to 50% flowering, achene yield, autogamy (%), hull content, volume weight and oil content. Twenty-three crosses showed significant positive sca effect for yield. Among the crosses, CMS-103A x EC-601978, CMS-10A x EC-601725, CMS-207AxEC-623023,P-89-1AxEC-623027, CMS-850AxEC-601878 andCMS-853AxEC-623027 exhibited higher positive sca effect for yield. Non additive component of the genetic variance was observed for majority of the traits studied.ICA

Randomized trial to compare efficacy of polyester mosquito net mesh with polypropylene mesh in inguinal hernia surgery in northwestern India

Objectives: Lichtenstein repair using mesh prosthesis is one of the most common repairs for inguinal hernia. In our country, the price of mesh contributes to a major portion of the total cost of the procedure. The study was conducted to compare the incidence of postoperative complications of using low-cost polyester mosquito net mesh with that of commercially available polypropylene mesh for inguinal hernia surgery by Lichtenstein repair and taking into consideration the cost of the operation. Methods: The study was a prospective randomized double-blind single-centre (government medical college) study. The study was conducted at SMS Medical College and Hospital, Jaipur, India, on 170 patients with inguinal hernia (45 bilateral and 125 unilateral) undergoing inguinal hernia surgery. Patients were randomly assigned to receive either of the meshes, and study of postoperative complications was carried out with follow-up of up to 1 year. The average cost of the procedure was also calculated. Chi-square test and Student's t-test were applied for significance testing (p-value and #8804; 0.05, significant; p-value < 0.001, highly significant). Results: No significant difference in incidence of postoperative complications between the two groups was observed. However, the cost of the operative procedure using the polyester net mesh was significantly lower (p-value < 0.0001). Conclusions: The results of the short-term follow-up clearly demonstrate that the incidence of postoperative complications from using polyester net mesh is well comparable with that of commercial polypropylene mesh. Using polyester mesh significantly reduces the cost of surgery and therefore it has a role in resource-limited settings. [Arch Clin Exp Surg 2016; 5(4.000): 200-205

Inheritance and Allelic Relationship among Gene(s) for White Rust Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss]

White rust [Albugo candida (Pers. Ex. Lev) Kuntze] is an important oomycetes disease of Indian mustard [Brassica juncea (L.) Czern & Coss] that causes a drastic reduction in seed yield and quality when the conditions are favorable. A set of 25 Indian mustard genotypes were screened against A. candida Delhi isolate (Ac-Dli) under both controlled and natural epiphytotic conditions. Out of 25, only six genotypes (Pusa Karishma, PDZ-3, Heera, BEC-144, BIO YSR, and Donskaja) were found highly resistant in both environments. To study the inheritance of resistance, four resistant genotypes (BEC-144, BIO YSR, Pusa Karishma, and Donskaja) were crossed with three susceptible genotypes (PM-24, Pusa Vijay, and MSTWR-17-15) in a definite design. The parents, F1, F2, and BC1F1 (F1 backcrossed with susceptible parent) generations were screened under both artificially controlled and natural epiphytotic conditions and the results indicated that the resistance in BIO YSR, BEC-144, and Pusa Karishma is governed by a single dominant gene, but more than one dominant gene is responsible for resistance in Donskaja. A test of an allelism conducted on the F2 population derived by crossing resistant (BEC-144) × resistant (BIO YSR) genotypes indicated that the gene imparting resistance to Ac-Dli isolate in the resistant parents BEC-144 and BIO YSR is the same and they are allelic to each other. Therefore, in broader terms, the information generated in the present study will be useful in Indian mustard breeding programs for the development of durable resistant cultivars