Development and characterization of a fibroblastic-like cell line from caudal fin of the red-line torpedo, Puntius denisonii (Day) (Teleostei: Cyprinidae)

A fibroblastic-like cell line was established from the ornamental ¢sh, red-line torpedo (Puntius denisonii). The red-line torpedo fin (RTF) cell line is being maintained in Leibovitz’s L-15 mediumsupplementedwith 10% fetal bovine serum (FBS) for over 1year at 28 1C on a continuous basis in normal atmosphere. The growth rate of RTF cells increased as the FBS proportion increased from 5% to 20% at 28 1C with optimum growth at the concentrations of 10% FBS. The morphology of RTF cell was predominantly fibroblastic like. Propagation of these cell lines was serum dependent, with a low plating efficiency (o15%). Karyotyping analysis of RTF cells at the 25th passage indicated that the modal chromosome number was 2n550. The cell line was cryopreserved in liquid nitrogen at �196 1C and could be recovered from storage after 6 months with good cell viability. Polymerase chain reaction amplification of a fragment of two mitochondrial genes, 16S rRNA and CO1, con¢rmed the identity of these cell lines with those reported from this animal species, confirming that the cell lines originated from P. denisonii. The bacterial extracellular products from Vibrio cholerae MTCC3904 and Aeromonas hydrophila were found to be toxic to RTF. The cell lines were not susceptible to viral nervous necrosis virus, a marine ¢sh virus

Electroslag remelting - Its status, mechanism and refining aspects in the production of quality steels

Electroslag remelting has been established as a new measure for the production of quality steels. Many properties of ESR steels are dramatically superior to those of conventionally produced steels. Majority of inclusions are eliminated during ESR depending on slag composition, temperature and melt rate. Usually, after ESR, the metal does not contain sany inclusion larger than 10-12 mm. The pronounced effect of ESR is a feasible and effective method of further enhancing the ductility and charpy U-notch toughness, with some improvement in strength as a result of refinement. ESR is a convenient and economic route for processing steels and alloys which are otherwise difficult to process

Iron making in ancient India - a critical assessment

It is a well known fact, that Indian craftsman produced quality iron products much earlier than the developed countries, making use of local reserves of iron ore. Iron produced in ancient India was mostly wrought iron. The metal was obtained in the form of a pasty mass and then shaped under hammer. Today, when India is producing several million tons of iron, primitive iron making is still exists in some parts of India. The primitive iron making furnaces declined in numbers gradually from about 500 in the beginning of 20'h century to about 150 by the middle of the century. Their present number is not known. Such furnaces are in operation deep into the forests and the tribal areas of India. The paper discusses briefly the history of iron making along with the critical assessment of the ancient processes. Some of the important measures absolutely. essential to upgrade the processes have also been discussed. Several pertinent modifications have been proposed in the design of these furnaces to make them more energy efficient and economically viable 'for tribal and rural populace

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Not AvailableThe experiment was conducted to assess the effect of different C/N ratios in bio floc systems on growth performances and digestive enzymatic activity of Litopenaeus vannamei. The experiment was carried out using inland ground saline water at 15 ppt salinity fortified with potassium and magnesium ions. Bio floc developed with different C/N ratios as treatments T1 (5:1), T2 (10:1), T3 (15:1), T4 (20:1) and T5 (25:1) respectively by manipulating the C/N content in feed and water by adding rice bran as carbon source and without bio floc used as control. After optimum floc produced in experimental tanks the juveniles Litopenaeus vannamei (avg wt. 3.37±0.03 gm) were stocked. At the end of the experiment the weight gain % and SGR was found higher (p<0.05) in treatments T3 (641.07c±12.55 and 3.29d±0.03) and T4 (650.00c±5.88 and 3.35d±0.01) respectively and in the treatment T1 and control group the growth rate was showed significantly similar (p > 0.05). A better FCR T4 (1.04a±0.01), T3 (1.12a±0.02) and T5 (1.16a±0.03) was observed significantly lower from control (2.23d±0.04) and T1 and T2. Similarly, significantly higher FER and PER was registered in T4 compared to control, T1 and T2 respectively. However, no significant difference in FER and PER was observed between T3 and T5. The 100% survival reported in treatments T1,T2, T3 and control groups with 99.33% and 94.33% survival reported in T4 and T5 groups.The different levels of C/N ratios in bio floc improved (p<0.05) the digestive enzymes activities in hepatopancreas and intestine like amylase, lipase, cellulose and protease in the treatment groups compared with control. The present study elucidates the suitability of optimum C/N ratios in bio floc for enhancing the growth and digestive enzyme activities in L. vannamei in inland ground saline water. The finding could help in reducing the environmental concern saline waste water discharge from the shrimp pond to the land.Not Availabl

Indigenous health practices of tribals in Ranchi District of Jharkhand

74-79An ethnobotanical survey was carried out among the ethnic groups in Ranchi district of Jharkhand. Traditional use of 41 plant species are documented in this study. These tribals are using 16 plant species to cure gastro-intestinal disorders, 7 each for headache as well as respiratory problems and 11 for the treatment of other health problems prevalent in the study area. India has a variety of tribal population reflecting its great ethnic diversity. Jharkhand is a homeland of 30 tribes including eight primitive tribes which constitute 28% of the total population. Majority of tribal population of Jharkhand lives in the forest ecosystem and has its own socio-cultural pattern and tradition. Tribal communities living in biodiversity rich areas possess a wealth of knowledge on the utilization and conservation of medicinal plants. They have developed this traditional knowledge over several years of observations, trial and error, inference and inheritance. Some of the indigenous teohnologies are really effective, much cheaper than modem medicines, prepared by locally available natural resources and easy to prepare. The potentiality of indigenous health technologies is increasingly being recognized. In present days, this useful knowledge of indigenous people is fast disappearing due to modernization and the tendency among younger generation to discard their traditional life style. There is an urgent need to study and document this precious knowledge for the posterity of human society. Keeping this in view, an ethnobotanical survey was carried out to explore the information regarding the medicinal use of indigenous plants by tribals found in adjoining forest and agricultural fields

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Not AvailableStudy on a meristic traits of Kumococius rodericensis Sorsogona tuberculata Platycephalus indicus and Rogadius serratus (family Platycephalidae), occurring along the East coast of India, was conducted to find easily differentiating traits. The study was based on the samples collected from landing centers situated along the East coast of India, during February 2015 to March 2016. A total of thirty-one morphometric and meristic characters were measured, counted and subjected to statistical analysis using software Statistica. Among eighteen morphometric measurements, five measurement (distance between upper and lower jaw, length of upper preopercular spine, snout length, maximum length of first dorsal spine, maximum length of second dorsal spine) did not show difference when subjected to factor analysis. Varimax raw factor rotation on factor loading 0.7 were selected for scatterplot of canonical score among root 1 and root 2, which revealed existence of distinct group of species. Number of gillrakers, spot on caudal fin, number of pored scale on lateral line and preopercular spine revealed significant variations among the species. The proportional measurements with their head length (HL) viz. Preorbital length: head length (POL:HL), Inter orbital width: head length (IOW:HL), and maximum eye diameter: head length (MED:HL) also revealed significant difference among the species. The study revealed certain morphological characters those can easily identify the flathead species.Not Availabl

Development of Quasi-crystal Reinforced Aluminium based Metal matrix Composites

This report presents the work carried out ot develop quasicrystal (QC) reinforced metal matric composites by casting route. The work involved preparation of stable Al62Cu25Fe13 alloy based quasicrystals by conventional casting route, and its incorporation in pure Al and Al-6.5Si alloy after pulverisation by ball milling. Two different particle size in the range -120+240 and -325+400 mesh were used for the present study. The study showed that smaller QC particles dissolved in the liquid Al metal during stirring and solidification

Development of microalloyed steels and its design aspects Part I : Medium and low carbon micro-alloyed forging steels

The development in microalloyed forging steels (MAFS) has undergone a revolution during last four decades. The extent of carbon, manganese, silicon and one or more microalloying element significantly affects the microstructure of the transformation product and in turn its mechanical properties strength and toughness. The processing conditions too play an important role in determining the mechanical properties of the final component. Microalloyed forging steels have found potential application in critical auto components leading to hyper productivity and overall cost reduction

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Not AvailableA new cell line [pearlspot fin (PSF)] has been developed from caudal fin of Etroplus suratensis, a brackish/freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum (FBS). The PSF cell line consisted predominantly of epithelial-like cells. The cells were able to grow at temperatures between 25 degrees C and 32 degrees C with optimum temperature of 28 degrees C. The growth rate of PSF cells increased as the FBS proportion increased from 2% to 20% at 28 degrees C with optimum growth at the concentration of 10% FBS. One marine fish virus (fish nodavirus) was tested on this cell line and found not susceptible. After confluency, the cells were subcultured with a split ratio of 1:2. The cells showed epithelial-like morphology and reached confluency on the third d after subculture. Polymerase chain reaction amplification of mitochondrial 16S rRNA and COI indicated identity of this cell line with those reported from this fish species, confirming that the cell line was of pearlspot origin. The cells were successfully cryopreserved and revived at the tenth, 25th, and 35th passages. The bacterial extracellular products from Vibrio cholerae MTCC 3904 were found to be toxic to PSF. Karyotyping analysis indicated that the modal chromosome number was 48.Not Availabl

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