Magreceptorok működésének genomszintü vizsgálata kromatin immunprecipitációval primer humán immun sejtekben = Decoding nuclear hormone receptor activity using chromatin immunoprecipitation in human primary immune cells

A kutatómunka során szisztematikusan feltérképeztük az un RXR heterodimer típusú receptorok szerepét a humán monocita eredetű dendritikus sejtek differenciálódása és immunfunkciói során. Ezek a receptorok a PPARg, LXR, VDR és RAR receptorok volta, melyeket kiegészítettünk az RXR receptorral is. A specikus utvonalak azonosítása mellett általános megállapításként elmondhattuk, hogy ez a receptorcsalad olyan molekuláris szenzorként működik, mely képes átprogramozni a sejt genkifejeződését külső és belső lipid szintek változása során. Összefüggést talaltunk az IL4 STAT6-on kerestüli szignalizációja és a PPARg receptor között és legújabban feltérképeztük a az RXR receptor genomi kötőhelyeit ls cisztromikus kölcsönhatásait is. | During our work we have systematically mapped the so called RXR heterodimeric receptors roles in monocyte derived dendritic cells during differentiation and immune function. These receptors included PPARg, LXR, VDR and RXR and also included their heterodimeric partner RXR. Based on the data obtained one can conclude that besides the specialized pathways identified, these receptors act as molecular sensors in detecting changing extra and intracellular lipid levels. We have also identified an interaction between IL4 mediated STAT6 signaling and PPARg and most recently we have detrained the RXR cistrome and its interactions

Magnetoresistance and structural study of electrodeposited Ni-Cu/Cu multilayers

Electrodeposition was used to produce Ni Cu/Cu multilayers by two-pulse plating (galvanostatic/potentiostatic control) from a single sulfate/sulfamate electrolyte at an optimized Cu deposition potential for the first time. Magnetoresistance measurements were carried out at room temperature for the Ni Cu/Cu multilayers as a function of the Ni Cu and Cu layer thicknesses and the electrolyte Cu2+ ion concentration. Multilayers with Cu layer thicknesses above 2 nm exhibited a giant magnetoresistance (GMR) effect with a dominating ferromagnetic contribution and with low saturation fields (below 1 kOe). A significant contribution from superparamagnetic (SPM) regions with high saturation fields occurred only for very small nominal magnetic layer thicknesses (around 1 nm). The presence of SPM regions was concluded from the GMR data also for thick magnetic layers with high Cu contents. This hints at a significant phase-separation in Ni-Cu alloys at low-temperature processing, in agreement with previous theoretical modeling and experiments. Low-temperature measurements performed on a selected multilayer down to 18 K indicated a strong increase of the GMR as compared to the room-temperature GMR. Structural studies of some multilayer deposits exhibiting GMR were performed by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The XRD patterns of Ni Cu/Cu multilayers exhibited in most cases clear satellite peaks, indicating a superlattice structure which was confirmed also by cross-sectional TEM. The deterioration of the multilayer structure revealed by XRD for high Cu-contents in the magnetic layer confirmed the phase-separation concluded from the GMR data

Circulating miRNA Profiling in Plasma Samples of Ovarian Cancer Patients

L

The effect of the presence of gold nanoparticles on the laser induced breakdown in argon gas

Noble metal nanoparticles can greatly affect the sensitivity and selectivity of many spectroscopic techniques, thus they are widely used in the analytical chemistry. In this current study we investigated the effects of the presence of gold nanoparticles on the formation of laserinduced breakdown plasmas in argon gas

The impact of delayed sample handling and type of anticoagulant on the interpretation of dysplastic signs detected by flow cytometry

Introduction: A growing body of evidence supports the usefulness of dysplastic signs detected by flow cytometry in the diagnosis of myelodysplastic syndromes (MDS). Our aim was to assess the impact of pre-analytical variables (delayed sample handling, type of anticoagulant, and different clones of antibody) in the interpretation of flow cytometric results. Material and methods: Bone marrow samples were labelled and analysed immediately after aspiration and on two consecutive days. The effect of anticoagulant type was evaluated in 16 bone marrow samples. Thirty-seven different immunophenotypic variables were recorded after eight-colour staining. Furthermore, 8 normal peripheral blood samples collected in K3-EDTA and Na-heparin were examined with different clones of CD11b antibodies and four parameters were recorded with both anticoagulants on two consecutive days. Results: Fourteen significant differences were detected in the initial immunophenotype of fresh samples collected in K3-EDTA and Na-heparin. Regardless of the anticoagulant type, eleven parameters remained stable despite delayed sample handling. Due to delayed sample processing, more alterations were detected in the samples collected in K3-EDTA than in the samples collected in Na-heparin. The type of CD11b clone influenced the reduction of fluorescence intensity only in samples collected in K3-EDTA, where the alterations were contrary to the changes observed in Na-heparin. Conclusions: Delayed sample processing causes considerable immunohenotypic alterations, which can lead to false interpretation of the results. If delayed sample evaluation is unavoidable, markers that remain more stable over time should be considered with more weight in the diagnosis of MDS

Signal Integration of IFN-I and IFN-II With TLR4 Involves Sequential Recruitment of STAT1-Complexes and NFkB to Enhance Pro-inflammatory Transcription

L

Specific enhancer selection by IRF3, IRF5 and IRF9 is determined by ISRE half-sites, 5' and 3' flanking bases, collaborating transcription factors and the chromatin environment in a combinatorial fashion

L