Sub-megahertz frequency stabilization of a diode 2 laser by digital laser current modulation

Digital laser current modulation (DLCM) is a convenient laser stabilization scheme whose major advantages are simplicity and inexpensiveness of implementation. However, there is a tradeoff between the SNR of the error signal and the laser linewidth due to the direct laser frequency modulation. In this paper, we demonstrated that DLCM can reduce the FWHM linewidth of a tunable diode laser down to 500 kHz using the modulation transfer spectrum of 2 line of a Li6 atomic vapor. For this purpose, a theoretical model is provided to analyze the DLCM-based modulation transfer spectrum. From the analysis, we experimentally explored the modulation effect on the DLCM spectrum to minimize the laser linewidth. Our result shows the optimized DLCM can stabilize a diode laser into the sub-megahertz regime without requiring acousto-optic and electro-optic modulators

Apyrase treatment prevents ischemia–reperfusion injury in rat lung isografts

ObjectiveEndothelial cells express the ectoenzyme ectonucleoside adenosine triphosphate diphosphohydrolase, an apyrase that inhibits vascular inflammation by catalyzing the hydrolysis of adenosine triphosphate and adenosine diphosphate. However, ectonucleoside adenosine triphosphate diphosphohydrolase expression is rapidly lost following oxidative stress, leading to the potential for adenosine triphosphate and related purigenic nucleotides to exacerbate acute solid organ inflammation and injury. We asked if administration of a soluble recombinant apyrase APT102 attenuates lung graft injury in a cold ischemia reperfusion model of rat syngeneic orthotopic lung transplantation.MethodsMale Fisher 344 donor lungs were cold preserved in a low-potassium dextrose solution in the presence or absence of APT102 for 18 hours prior to transplantation into syngeneic male Fisher 344 recipients. Seven minutes after reperfusion, lung transplant recipients received either a bolus of APT102 or vehicle (saline solution). Four hours after reperfusion, APT102- and saline solution–treated groups were evaluated for lung graft function and inflammation.ResultsAPT102 significantly reduced lung graft extracellular pools of adenosine triphosphate and adenosine diphosphate, improved oxygenation, and protected against pulmonary edema. Apyrase treatment was associated with attenuated neutrophil graft sequestration and less evidence of tissue inflammation as assessed by myeloperoxidase activity, expression of proinflammatory mediators, and numbers of apoptotic endothelial cells.ConclusionsAdministration of a soluble recombinant apyrase promotes lung function and limits the tissue damage induced by prolonged cold storage, indicating that extracellular purigenic nucleotides play a key role in promoting ischemia–reperfusion injury following lung transplantation