Agrobacterium tumefaciens Deploys a Superfamily of Type VI Secretion DNase Effectors as Weapons for Interbacterial Competition In Planta

The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many Proteobacteria to target effectors/toxins into both eukaryotic and prokaryotic cells. We report that Agrobacterium tumefaciens, a soil bacterium that triggers tumorigenesis in plants, produces a family of type VI DNase effectors (Tde) that are distinct from previously known polymorphic toxins and nucleases. Tde exhibits an antibacterial DNase activity that relies on a conserved HxxD motif and can be counteracted by a cognate immunity protein, Tdi. In vitro, A. tumefaciens T6SS could kill Escherichia coli but triggered a lethal counterattack by Pseudomonas aeruginosa upon injection of the Tde toxins. However, in an in planta coinfection assay, A. tumefaciens used Tde effectors to attack both siblings cells and P. aeruginosa to ultimately gain a competitive advantage. Such acquired T6SS-dependent fitness in vivo and conservation of Tde-Tdi couples in bacteria highlights a widespread antibacterial weapon beneficial for niche colonization

Two-dimensional viscous flow computations of hypersonic scramjet nozzle flowfields at design and off-design conditions

The PARC2D code has been selected to analyze the flowfields of a representative hypersonic scramjet nozzle over a range of flight conditions from Mach 3 to 20. The flowfields, wall pressures, wall skin friction values, heat transfer values and overall nozzle performance are presented

Strange nonchaotic attractors in noise driven systems

Strange nonchaotic attractors (SNAs) in noise driven systems are investigated. Before the transition to chaos, due to the effect of noise, a typical trajectory will wander between the periodic attractor and its nearby chaotic saddle in an intermittent way, forms a strange attractor gradually. The existence of SNAs is confirmed by simulation results of various critera both in map and continuous systems. Dimension transition is found and intermittent behavior is studied by peoperties of local Lyapunov exponent. The universality and generalization of this kind of SNAs are discussed and common features are concluded

Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei

BACKGROUND Vacuolar H-ATPase (V-ATPase) is a highly conserved protein complex which hydrolyzes ATP and pumps protons to acidify vacuolar vesicles. Beyond its role in pH maintenance, the involvement of V-ATPase in endocytosis is well documented in mammals and plants but is less clear in Trypanosoma brucei. METHODS In this study, the subcellular localization of V-ATPase subunit B (TbVAB) of T. brucei was assessed via in situ N-terminal YFP-tagging and immunofluorescence assays. Transgenic bloodstream forms (BSF) of T. brucei were generated which comprised either a V-ATPase subunit B (TbVAB) conditional knockout or a V-ATPase subunit A (TbVAA) knockdown. Acridine orange and BCECF-AM were employed to assess the roles of V-ATPase in the pH regulation of BSF T. brucei. The endocytic activities of three markers were also characterized by flow cytometry analyses. Furthermore, trypanosomes were counted from trypanolysis treatment groups (either containing 1% or 5% NHS) and endocytosed trypanosome lytic factor (TLF) was also analyzed by an immunoblotting assay. RESULTS TbVAB was found to localize to acidocalcisomes, lysosomes and probably also to endosomes of BSF of T. brucei and was demonstrated to be essential for cell growth. TbVAB depletion neutralized acidic organelles at 24 hours post-tetracycline depletion (hpd), meanwhile the steady state intracellular pH increased from 7.016 ± 0.013 to 7.422 ± 0.058. Trypanosomes with TbVAB depletion at 24 hpd were found to take up more transferrin (2.068 ± 0.277 fold) but less tomato lectin (49.31 ± 22.57%) by endocytosis, while no significant change was detected in dextran uptake. Similar endocytic dysregulated phenotypes were also observed in TbVAA knockdown cells. In addition, TbVAB depleted trypanosomes showed a low uptake of TLF and exhibited less sensitive to lysis in both 1% and 5% NHS treatments. CONCLUSIONS TbVAB is a key component of V-ATPase and was found to play a key function in endocytosis as well as exhibiting different effects in a receptor/cargo dependent manner in BSF of T. brucei. Besides vacuolar alkalinization, the dysregulation of endocytosis in TbVAB depleted T. brucei is considered to contribute to the reduced sensitivity to lysis by normal human serum

Lorentz Symmetry and the Internal Structure of the Nucleon

To investigate the internal structure of the nucleon, it is useful to introduce quantities that do not transform properly under Lorentz symmetry, such as the four-momentum of the quarks in the nucleon, the amount of the nucleon spin contributed by quark spin, etc. In this paper, we discuss to what extent these quantities do provide Lorentz-invariant descriptions of the nucleon structure.Comment: 6 pages, no figur

General-relativistic coupling between orbital motion and internal degrees of freedom for inspiraling binary neutron stars

We analyze the coupling between the internal degrees of freedom of neutron stars in a close binary, and the stars' orbital motion. Our analysis is based on the method of matched asymptotic expansions and is valid to all orders in the strength of internal gravity in each star, but is perturbative in the ``tidal expansion parameter'' (stellar radius)/(orbital separation). At first order in the tidal expansion parameter, we show that the internal structure of each star is unaffected by its companion, in agreement with post-1-Newtonian results of Wiseman (gr-qc/9704018). We also show that relativistic interactions that scale as higher powers of the tidal expansion parameter produce qualitatively similar effects to their Newtonian counterparts: there are corrections to the Newtonian tidal distortion of each star, both of which occur at third order in the tidal expansion parameter, and there are corrections to the Newtonian decrease in central density of each star (Newtonian ``tidal stabilization''), both of which are sixth order in the tidal expansion parameter. There are additional interactions with no Newtonian analogs, but these do not change the central density of each star up to sixth order in the tidal expansion parameter. These results, in combination with previous analyses of Newtonian tidal interactions, indicate that (i) there are no large general-relativistic crushing forces that could cause the stars to collapse to black holes prior to the dynamical orbital instability, and (ii) the conventional wisdom with respect to coalescing binary neutron stars as sources of gravitational-wave bursts is correct: namely, the finite-stellar-size corrections to the gravitational waveform will be unimportant for the purpose of detecting the coalescences.Comment: 22 pages, 2 figures. Replaced 13 July: proof corrected, result unchange

Quasi-Local Energy Flux of Spacetime Perturbation

A general expression for quasi-local energy flux for spacetime perturbation is derived from covariant Hamiltonian formulation using functional differentiability and symplectic structure invariance, which is independent of the choice of the canonical variables and the possible boundary terms one initially puts into the Lagrangian in the diffeomorphism invariant theories. The energy flux expression depends on a displacement vector field and the 2-surface under consideration. We apply and test the expression in Vaidya spacetime. At null infinity the expression leads to the Bondi type energy flux obtained by Lindquist, Schwartz and Misner. On dynamical horizons with a particular choice of the displacement vector, it gives the area balance law obtained by Ashtekar and Krishnan.Comment: 8 pages, added appendix, version to appear in Phys. Rev.