NUTRITIONAL AND PHYTOCHEMICAL ASSESMENT OF WILD EDIBLE FRUIT OF AEGLE MARMELOS (LINN.) USED BY THE TRIBES OF BHIWAPUR TAHSIL NAGPUR DISTRICT, INDIA

Objectives: Wild edible plants play an important role in human life and are the vital constituent of the traditional diet. People of the Bhiwapur Tahsilare very close to the nature, wild fruits like Aegle marmelos (Bael), which is one of the natural resources in the Tahsil. They have a direct dependence onthe wild plants for their sustenance. Owing to the easy accessibility, the fruits are very commonly utilized by the tribal populations and travelers.Thepresent investigation aimed to assess the nutritional and phytochemical analysis of ripened fruits of a popularly known medicinal plant A. marmelose.Methods: The study includes the estimation of ash content, protein, carbohydrate (sugar), vitamins and mineral contents (Cu, Fe, Mn, Zn, Ca, Na, K)of Bael fruit. The water extract was screened for the qualitative phytochemical analysis.Results: The fresh fruits were found a rich source of protein (6.91±0.11 g/100 g), carbohydrate (22.55±0.15 g/100 g) fiber (7.26±0.23 g/100 g) andenergy (133.14 Kcal/100 g). The fruits were found to contain high calcium (86.69±0.01 mg/100 g) content and may be considered as a rich sourceto facilitate the rehabilitation of bone problems in the human being. The Na/K and Zn/Cu ratio were found to be 0.012 and 12.26 may attribute themedicinal properties in cardiovascular disorders. The water extract showed the presence of carbohydrates, proteins, sterols, alkaloids, glycosides,saponins, polyphenols, and flavonoids.Conclusion: The nutritional and phytochemical analysis reveals that the fruits are not only acting as supplementary foods, but is the tonic requirementsof the tribal's and deprived of poor Bhiwapur Tahsil.Keywords: Aegle marmelos, Bhiwapur, nutritional, Phytochemical, Tribal

Spherically symmetric Einstein-Maxwell theory and loop quantum gravity corrections

Effects of inverse triad corrections and (point) holonomy corrections, occuring in loop quantum gravity, are considered on the properties of Reissner-Nordstr\"om black holes. The version of inverse triad corrections with unmodified constraint algebra reveals the possibility of occurrence of three horizons (over a finite range of mass) and also shows a mass threshold beyond which the inner horizon disappears. For the version with modified constraint algebra, coordinate transformations are no longer a good symmetry. The covariance property of spacetime is regained by using a \emph{quantum} notion of mapping from phase space to spacetime. The resulting quantum effects in both versions of these corrections can be associated with renormalization of either mass, charge or wave function. In neither of the versions, Newton's constant is renormalized. (Point) Holonomy corrections are shown to preclude the undeformed version of constraint algebra as also a static solution, though time-independent solutions exist. A possible reason for difficulty in constructing a covariant metric for these corrections is highlighted. Furthermore, the deformed algebra with holonomy corrections is shown to imply signature change.Comment: 38 pages, 9 figures, matches published versio

From the discrete to the continuous - towards a cylindrically consistent dynamics

Discrete models usually represent approximations to continuum physics. Cylindrical consistency provides a framework in which discretizations mirror exactly the continuum limit. Being a standard tool for the kinematics of loop quantum gravity we propose a coarse graining procedure that aims at constructing a cylindrically consistent dynamics in the form of transition amplitudes and Hamilton's principal functions. The coarse graining procedure, which is motivated by tensor network renormalization methods, provides a systematic approximation scheme towards this end. A crucial role in this coarse graining scheme is played by embedding maps that allow the interpretation of discrete boundary data as continuum configurations. These embedding maps should be selected according to the dynamics of the system, as a choice of embedding maps will determine a truncation of the renormalization flow.Comment: 22 page

LHC Magnet Tests: Operational Techniques and Empowerment for Successful Completion

The LHC magnet tests operation team developed various innovative techniques, particularly since early 2004, to complete the superconductor magnet tests by Feb. 2007. Overall and cryogenic priority handling, rapid on-bench thermal cycling, rule-based goodness evaluation on round-the-clock basis, multiple, mashed web systems are some of these techniques applied with rigour for successful tests completion in time. This paper highlights these operation empowerment tools which had a pivotal role for success. A priority handling method was put in place to enable maximum throughput from twelve test benches, having many different constraints. For the cryogenics infrastructure, it implied judicious allocation of limited resources to the benches. Rapid On-Bench Thermal Cycle was a key strategy to accelerate magnets tests throughput, saving time and simplifying logistics. First level magnet appraisal was developed for 24 hr decision making so as to prepare a magnet further for LHC or keep it on standby. Web based systems (Tests Management and E-Traveller) were other essential ideas to track & coordinate various stages of tests handled by different teams

Loop Quantum Cosmology: A Status Report

The goal of this article is to provide an overview of the current state of the art in loop quantum cosmology for three sets of audiences: young researchers interested in entering this area; the quantum gravity community in general; and, cosmologists who wish to apply loop quantum cosmology to probe modifications in the standard paradigm of the early universe. An effort has been made to streamline the material so that, as described at the end of section I, each of these communities can read only the sections they are most interested in, without a loss of continuity.Comment: 138 pages, 15 figures. Invited Topical Review, To appear in Classical and Quantum Gravity. Typos corrected, clarifications and references adde

Novel internal regulators and candidate miRNAs within miR-379/miR-656 miRNA cluster can alter cellular phenotype of human glioblastoma

Clustered miRNAs can affect functioning of downstream pathways due to possible coordinated function. We observed 78-88% of the miR-379/miR-656 cluster (C14MC) miRNAs were downregulated in three sub-types of diffuse gliomas, which was also corroborated with analysis from The Cancer Genome Atlas (TCGA) datasets. The miRNA expression levels decreased with increasing tumor grade, indicating this downregulation as an early event in gliomagenesis. Higher expression of the C14MC miRNAs significantly improved glioblastioma prognosis (Pearson’s r=0.62; p<3.08e-22). ENCODE meta-data analysis, followed by reporter assays validated existence of two novel internal regulators within C14MC. CRISPR activation of the most efficient internal regulator specifically induced members of the downstream miRNA sub-cluster and apoptosis in glioblastoma cells. Luciferase assays validated novel targets for miR-134 and miR-485-5p, two miRNAs from C14MC with the most number of target genes relevant for glioma. Overexpression of miR-134 and miR-485-5p in human glioblastoma cells suppressed invasion and proliferation, respectively. Furthermore, apoptosis was induced by both miRs, individually and in combination. The results emphasize the tumor suppressive role of C14MC in diffuse gliomas, and identifies two specific miRNAs with potential therapeutic value and towards better disease management and therapy

Antioxidant rich flavonoids from Oreocnide integrifolia enhance glucose uptake and insulin secretion and protects pancreatic β-cells from streptozotocin insult

<p>Abstract</p> <p>Background</p> <p>Insulin deficiency is the prime basis of all diabetic manifestations and agents that can bring about insulin secretion would be of pivotal significance for cure of diabetes. To test this hypothesis, we carried out bioactivity guided fractionation of <it>Oreocnide integrifolia </it>(Urticaceae); a folklore plant consumed for ameliorating diabetic symptoms using experimental models.</p> <p>Methods</p> <p>We carried out bioassay guided fractionation using RINmF and C2C12 cell line for glucose stimulated insulin secretion (GSIS) and glucose uptake potential of fractions. Further, the bioactive fraction was challenged for its GSIS in cultured mouse islets with basal (4.5 mM) and stimulated (16.7 mM) levels of glucose concentrations. The Flavonoid rich fraction (FRF) was exposed to 2 mM streptozotocin stress and the anti-ROS/RNS potential was evaluated. Additionally, the bioactive fraction was assessed for its antidiabetic and anti-apoptotic property <it>in-vivo </it>using multidose streptozotocin induced diabetes in BALB/c mice.</p> <p>Results</p> <p>The results suggested FRF to be the most active fraction as assessed by GSIS in RINm5F cells and its ability for glucose uptake in C2C12 cells. FRF displayed significant potential in terms of increasing intracellular calcium and cAMP levels even in presence of a phosphodiesterase inhibitor, IBMX in cultured pancreatic islets. FRF depicted a dose-dependent reversal of all the cytotoxic manifestations except peroxynitrite and NO formation when subjected <it>in-vitro </it>along with STZ. Further scrutinization of FRF for its <it>in-vivo </it>antidiabetic property demonstrated improved glycemic indices and decreased pancreatic β-cell apoptosis.</p> <p>Conclusions</p> <p>Overall, the flavonoid mixture has shown to have significant insulin secretogogue, insulinomimetic and cytoprotective effects and can be evaluated for clinical trials as a therapeutant in the management of diabetic manifestations.</p