Influence of transcutaneous vagus nerve stimulation on cardiac vagal activity: Not different from sham stimulation and no effect of stimulation intensity

© 2019 Borges et al. The present study investigated the effects of transcutaneous vagus nerve stimulation on cardiac vagal activity, the activity of the vagus nerve regulating cardiac functioning. We applied stimulation on the left cymba conchae and tested the effects of different stimulation intensities on a vagally-mediated heart rate variability parameter (i.e., the root mean square of successive differences) as well as on subjective ratings of strength of perceived stimulation intensity and unpleasantness due to the stimulation. Three experiments (within-subject designs, M = 61 healthy participants each) were carried out: In Experiment 1, to choose one fixed stimulation intensity for the subsequent studies, we compared three preset stimulation intensities (i.e., 0.5, 1.0 and 1.5 mA) with each other. In Experiment 2, we compared the set stimulation method with the free stimulation method, in which the participants were instructed to freely choose an intensity. In Experiment 3, to control for placebo effects, we compared both methods (i.e., set stimulation vs. free stimulation) with their respective sham stimulations. In the three experiments, an increase of cardiac vagal activity was found from resting to the stimulation phases. However, this increase in cardiac vagal activity was not dependent on stimulation intensity (Experiment 1), the method used to stimulate (i.e., set vs. free; Experiment 2), or whether stimulation was active or sham (Experiment 3). This pattern of results was solidly supported by Bayesian estimations. On the subjective level, higher stimulation intensities were perceived as significantly stronger and a stronger stimulation was generally also perceived as more unpleasant. The results suggest that cardiac vagal activity may be similarly influenced by afferent vagal stimuli triggered by active and sham stimulation with different stimulation intensities. Potential explanations for these findings and its implications for future research with tVNS are discussed

Transcutaneous Vagus Nerve Stimulation May Enhance Only Specific Aspects of the Core Executive Functions. A Randomized Crossover Trial

Background: Individuals are able to perform goal-directed behaviors thanks to executive functions. According to the neurovisceral integration model, executive functions are upregulated by brain areas such as the prefrontal and cingulate cortices, which are also crucially involved in controlling cardiac vagal activity. An array of neuroimaging studies already showed that these same brain areas are activated by transcutaneous vagus nerve stimulation (tVNS). Despite evidence towards effects of tVNS on specific executive functions such as inhibitory control, there have been no studies investigating what type of inhibition is improved by tVNS by systematically addressing them within the same experiment. Furthermore, the effect of tVNS on another core executive function, cognitive flexibility, has not yet been investigated. Objective: We investigated the effects of tVNS on core executive functions such as inhibitory control and cognitive flexibility. Methods: Thirty-two participants (nine women, Mage = 23.17) took part in this study. Vagally-mediated heart rate variability parameters (root mean square of successive differences, RMSSD, and high frequency, HF) were measured while participants performed four different cognitive tasks that mainly rely on different aspects of both the aforementioned executive functions. Results: Despite clear conflict effects in the four tasks, only performance on the task used to measure set-shifting paradigm was improved by tVNS, with switch costs being lower during tVNS than during sham stimulation. Furthermore, HF increased during each of the cognitive flexibility tasks, although HF during tVNS did not differ from HF during sham stimulation. Conclusion: The results indicate for the first time a) that tVNS can increase cognitive flexibility in a set-shifting paradigm, and b) that tVNS may exert a stronger effect on cognitive flexibility than inhibition. The present study provides only partial evidence for the neurovisceral integration model. Future studies should address further paradigms that demand cognitive flexibility, thus investigating this new hypothesis on the specificity of the tVNS effects on cognitive flexibility

The diving response and cardiac vagal activity: A systematic review and meta‐analysis

This article aimed to synthesize the various triggers of the diving response and to perform a meta-analysis assessing their effects on cardiac vagal activity. The protocol was preregistered on PROSPERO (CRD42021231419; 01.07.2021). A systematic and meta-analytic review of cardiac vagal activity was conducted, indexed with the root mean square of successive differences (RMSSD) in the context of the diving response. The search on MEDLINE (via PubMed), Web of Science, ProQuest and PsycNet was finalized on November 6th, 2021. Studies with human participants were considered, measuring RMSSD pre- and during and/or post-exposure to at least one trigger of the diving response. Seventeen papers (n = 311) met inclusion criteria. Triggers examined include face immersion or cooling, SCUBA diving, and total body immersion into water. Compared to resting conditions, a significant moderate to large positive effect was found for RMSSD during exposure (Hedges' g = 0.59, 95% CI 0.36 to 0.82, p < .001), but not post-exposure (g = 0.11, 95% CI −0.14 to 0.36, p = .34). Among the considered moderators, total body immersion had a significantly larger effect than forehead cooling (QM = 23.46, df = 1, p < .001). No further differences were detected. Limitations were the small number of studies included, heterogenous triggers, few participants and low quality of evidence. Further research is needed to investigate the role of cardiac sympathetic activity and of the moderators

Forage Quality of Mixtures of \u3ci\u3eBromus wildenowii\u3c/i\u3e and \u3ci\u3eB. parodii\u3c/i\u3e Influenced by Nitrogen Fertilization or Alfalfa Interseeding

Under good soil fertility conditions, Bromus wildenowii Kunth and Bromus parodii Covas et Itria are two high producing forage grasses. The objective of this study was to evaluate the effect of nitrogen fertilization or the association with alfalfa (Medicago sativa L) on yield and forage quality of mixtures of both grasses during the establishment year. Nitrogen fertilizer (300 kg ha-1) was broadcasted in three 100 kg N dosis. Nitrogen fertilization or alfalfa intercropping did not increase dry matter yield. Tiller density and crude protein content of grass forage were increased by nitrogen application. Intercropped alfalfa increased N content of grasses mixture. In vitro dry matter disappearance, neutral detergent fiber and lignin content were not affected by treatments

Coping related variables, cardiac vagal activity and working memory performance under pressure

The aim of this study was to assess the predictive role of coping related variables (trait emotional intelligence and reinvestment, challenge and threat appraisals and cardiac vagal activity) on cardiac vagal activity and working memory under low pressure (LP) and high pressure (HP) conditions. Participants (n = 49) completed trait questionnaires, the Decision Specific Reinvestment Scale, the Movement Specific Reinvestment Scale and Trait Emotional Intelligence Questionnaire. They realized the automated span task, which tests working memory, under counterbalanced LP and HP conditions. Cardiac vagal activity measurements were taken at rest, task and post task for 5 min, along with self-reported ratings of stress. Upon completion of the task, self-report measures of motivation, stress appraisal, attention and perceived pressure were completed. Current findings suggest cardiac vagal activity at rest can predict cardiac vagal activity under pressure, decision reinvestment influences cardiac vagal activity in cognitive tasks under LP and working memory performance is predicted by task cardiac vagal activity in HP only. These results show the importance of combining both subjective and objective psychophysiological variables in performance prediction and strengthen the need for this approach to be adopted across samples

Transcutaneous vagus nerve stimulation via tragus or cymba conchae: Are its psychophysiological effects dependent on the stimulation area?

Efforts in optimizing transcutaneous vagus nerve stimulation (tVNS) are crucial to further develop its potential in improving cognitive and autonomic regulation. The present study focused on this topic. The aim was to compare for the first time the main stimulation areas of the ear currently used in studies with tVNS, taking cognitive as well as neurophysiological effects into account. The main areas to be compared with one another were tragus, cymba conchae, and earlobe (sham) stimulation. Post-error slowing, which has already been shown to be influenced by tVNS, was used to investigate the cognitive effects of tVNS when applied on the different auricular areas. On the neurophysiological level, we measured pupillary responses as an index of norepinephrine activity during post-error slowing, and cardiac vagal activity to investigate the activation of neural pathways involved in post-error slowing. Stimulation of different auricular areas led to no differences in post-error slowing and in pupillary responses. However, the neurological processes involved in post-error slowing could be observed, since norepinephrine activity increased after committing an error. Further, there was an increase in cardiac vagal activity over the test period that was independent of the stimulation areas. The results suggest that tVNS targeting the ear might have a non-specific effect on the processing of error commission, on pupillary responses, and on cardiac vagal activity. We conclude that it is necessary to consider alternatives for sham conditions other than electrical earlobe stimulation. [Abstract copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Effective density of Aquadag and fullerene soot black carbon reference materials used for SP2 calibration

The mass and effective density of black carbon (BC) particles generated from aqueous suspensions of Aquadag and fullerene soot was measured and parametrized as a function of their mobility diameter. The measurements were made by two independent research groups by operating a differential mobility analyser (DMA) in series with an aerosol particle mass analyser (APM) or a Couette centrifugal particle mass analyser (CPMA). Consistent and reproducible results were found in this study for different production lots of Aquadag, indicating that the effective density of these particles is a stable quantity and largely unaffected by differences in aerosol generation procedures and suspension treatments. The effective density of fullerene soot particles from one production lot was also found to be stable and independent of suspension treatments. Some differences to previous literature data were observed for both Aquadag and fullerene soot at larger particle diameters. Knowledge of the exact relationship between mobility diameter and particle mass is of great importance, as DMAs are commonly used to size-select particles from BC reference materials for calibration of single particle soot photometers (SP2), which quantitatively detect the BC mass in single particles