Online Game Addiction and the Level of Depression Among Adolescents in Manila, Philippines

Introduction: World Health Organization recognizes online game addiction as a mental health condition. The rise of excessive online gaming is emerging in the Philippines, with 29.9 million gamers recorded in the country. The incidence of depression is also increasing in the country. The current correlational analysis evaluated the association between online game addiction and depression in Filipino adolescents. Methods: A paper-and-pencil self-administered questionnaire assessing depression and online game addiction was distributed from August to November, 2018. The questionnaire included socio-demographic profiles of the respondents, and the 14-item Video Game Addiction Test (VAT) (Cronbach's ?=0.91) and the Patient Health Questionnaire-9 (Cronbach's ?=0.88) to determine levels of online game addiction and depression, respectively. Multiple regression analyses were used to test the association between depression and online game addiction. Results: Three hundred adolescents (59% males, 41% females) participated in the study. Fifty-three out of 300 respondents (12.0% males, 5.7% females) had high level of online game addiction as reflected in their high VAT scores. In this study, 37 respondents (6.7% males, 5.7% females) had moderately severe depression and 6 (2.0%) females had severe depression. Online game addiction was positively correlated with depression in this study (r=0.31; p<0.001). When multiple regression analysis was computed, depression was found to be a predictor of online game addiction (Coefficient=0.0121; 95% CI-8.1924 - 0.0242; p=0.05). Conclusion: Depression, as associated with online game addiction, is a serious threat that needs to be addressed. High level of online game addiction, as positively correlated to the rate of depression among adolescents in Manila, could potentially be attributed to the booming internet industry and lack of suffiicent mental health interventions in the country. Recommended interventions include strengthening depression management among adolescents and improving mental health services for this vulnerable population groups in schools and within the communities

Development of an Animal Microbiota Online Web-Based System Basilio: Bacterial Assemblages and Species Identity Library Online

Zoonotic diseases caused by infectious microorganisms transmitted from animals to humans, are becoming a greater threat to global health security. With the massive amount of data on animal microbiota and zoonosis research that recognize the pivotal roles of microorganisms in animal physiology and human health, it is necessary to process and present the data and make them accessible to researchers, educators, and to those involved in microbiota and zoonotic infections studies. The Bacterial Assemblages and Species Identity Library Online (BASILIO) is a web-based bacterial system and database that was created to curate data from various research publications on isolated bacteria from a variety of animal species worldwide. The system focuses on the presentation of animal and bacterial taxonomy, with an emphasis on the abundance of bacterial diversity in the animals studied and the classification of bacteria according to their pathogenicity. The project gathered data on animal microbiota from published studies in reputable peer-reviewed journals. AGILE development approach was utilized to develop the information system iteratively. Unit, system, and acceptability testing were used to execute test plans. The BASILIO web-system was evaluated using ISO 9126 standards and found to be functional, usable, reliable, efficient, portable, maintainable, and usable. The BASILIO in its current state, fills in the need for a database dedicated to fast access to information on pathogenic bacteria found in animals. Future developers are proposed to adopt the BASILIO system and incorporate other data management tools and algorithms to create a more complex and robust system

Antimicrobial Activity of the Rhizospheric Bacillus Species Isolated from Potato (Solanum tuberosum) Organic Farm Soils in the Philippines

The purpose of this study is to determine the potential of rhizospheric bacteria belonging to the genus Bacillus isolated from the organic soil of Solanum tuberosum (potato) as an untapped and promising source of novel antimicrobials to combat infections, particularly multidrug-resistant strains. The rhizospheric Bacillus species were isolated using serial dilution and aerobic cultivation. Hydrolytic exoenzyme production was determined using plate techniques, whereas antimicrobial activity was determined using the cross-streak method and agar-disc diffusion assay. The data indicate that the Bacillus isolates possess antimicrobial property against gram-positive bacterial pathogens. The activities were compared to those of the antibiotic Rifampicin as a control. Notably, several Bacillus isolates inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). The top performing Bacillus isolates were identified by 16s rRNA gene sequence analysis, which showed the similarities of the isolates to known soil-associated and plant-growth-promoting species; B. velezensis, B. mojavensis, B. subtilis, B. sonorensis, B. tequilensis, B. clausii, B. amyloliquefaciens, B. altitudinis, and B. siamensis from those sequences available in GENBANK. The present investigation establishes the presence of antagonistic Bacillus species in S. tuberosum's rhizosphere. The findings may form the basis for further investigation of the active compounds produced by the isolates and the mechanisms underlying their antimicrobial activity, while optimizing the culture medium for efficient production of potent antimicrobial compounds to combat infectious agents may further be investigated

Antimicrobial Activity of the Rhizospheric Bacillus Species Isolated from Potato (Solanum tuberosum) Organic Farm Soils in the Philippines

The purpose of this study is to determine the potential of rhizospheric bacteria belonging to the genus Bacillus isolated from the organic soil of Solanum tuberosum (potato) as an untapped and promising source of novel antimicrobials to combat infections, particularly multidrug-resistant strains. The rhizospheric Bacillus species were isolated using serial dilution and aerobic cultivation. Hydrolytic exoenzyme production was determined using plate techniques, whereas antimicrobial activity was determined using the cross-streak method and agar-disc diffusion assay. The data indicate that the Bacillus isolates possess antimicrobial property against gram-positive bacterial pathogens. The activities were compared to those of the antibiotic Rifampicin as a control. Notably, several Bacillus isolates inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). The top performing Bacillus isolates were identified by 16s rRNA gene sequence analysis, which showed the similarities of the isolates to known soil-associated and plant-growth-promoting species; B. velezensis, B. mojavensis, B. subtilis, B. sonorensis, B. tequilensis, B. clausii, B. amyloliquefaciens, B. altitudinis, and B. siamensis from those sequences available in GENBANK. The present investigation establishes the presence of antagonistic Bacillus species in S. tuberosum's rhizosphere. The findings may form the basis for further investigation of the active compounds produced by the isolates and the mechanisms underlying their antimicrobial activity, while optimizing the culture medium for efficient production of potent antimicrobial compounds to combat infectious agents may further be investigated

Community-based surveillance of in the indigenous community of Boliwong, Philippines: from April to December 2017

OBJECTIVES For the first time, Boliwong, an indigenous community in the Philippines, was surveyed for the prevalence of Cryptosporidium from April to December 2017. METHODS Cryptosporidium oocysts were detected in samples from the river, creek, and water pumps via immunomagnetic separation techniques, and from human and animal concentrated faecal samples using the modified Ziehl-Neelsen technique. RESULTS Seven of the 24 water samples (29.2%) were positive for Cryptosporidium, with the highest concentration (0.8 oocyst/L) detected in the creek. Of 35 fecal samples from different animal groups, 8 (21.6%) were positive for Cryptosporidium oocysts. The highest intensity of oocyst shedding was detected in dogs (χ2 =8.00). Of the 137 human fecal samples, 39 (28.5%) were infected with Cryptosporidium. In this study, 3 risk factors were found to be associated with infection: (1) location (crude odds ratio [cOR], 16.39; 95% confidence interval [CI], 2.11 to 127.41; p=0.008), (2) drinking water from the natural spring (cOR, 0.29; 95% CI, 0.11 to 0.82; p<0.05), and (3) using an open pit as a sanitary toilet facility (cOR, 2.44; 95% CI, 1.14 to 5.20; p<0.05). When the cOR was adjusted, using an open pit as a sanitary toilet facility remained a significant risk factor of infection (adjusted OR, 0.41; 95% CI, 0.19 to 0.90; p<0.05). CONCLUSIONS There is a potentially emerging Cryptosporidium zoonosis in Boliwong, Lagawe, Philippines. It is recommended that the toilet facilities and the water system in the community be rehabilitated to avoid any possible disease outbreak. Health education is also needed in the community to maintain proper hygiene and sanitation practices

Development of an Animal Microbiota Online Web-Based System Basilio: Bacterial Assemblages and Species Identity Library Online

Zoonotic diseases caused by infectious microorganisms transmitted from animals to humans, are becoming a greater threat to global health security. With the massive amount of data on animal microbiota and zoonosis research that recognize the pivotal roles of microorganisms in animal physiology and human health, it is necessary to process and present the data and make them accessible to researchers, educators, and to those involved in microbiota and zoonotic infections studies. The Bacterial Assemblages and Species Identity Library Online (BASILIO) is a web-based bacterial system and database that was created to curate data from various research publications on isolated bacteria from a variety of animal species worldwide. The system focuses on the presentation of animal and bacterial taxonomy, with an emphasis on the abundance of bacterial diversity in the animals studied and the classification of bacteria according to their pathogenicity. The project gathered data on animal microbiota from published studies in reputable peer-reviewed journals. AGILE development approach was utilized to develop the information system iteratively. Unit, system, and acceptability testing were used to execute test plans. The BASILIO web-system was evaluated using ISO 9126 standards and found to be functional, usable, reliable, efficient, portable, maintainable, and usable. The BASILIO in its current state, fills in the need for a database dedicated to fast access to information on pathogenic bacteria found in animals. Future developers are proposed to adopt the BASILIO system and incorporate other data management tools and algorithms to create a more complex and robust system