Biodiversity of Prokaryotic Communities Associated with the Ectoderm of Ectopleura crocea (Cnidaria, Hydrozoa)

The surface of many marine organisms is colonized by complex communities of microbes, yet our understanding of the diversity and role of host-associated microbes is still limited. We investigated the association between Ectopleura crocea (a colonial hydroid distributed worldwide in temperate waters) and prokaryotic assemblages colonizing the hydranth surface. We used, for the first time on a marine hydroid, a combination of electron and epifluorescence microscopy and 16S rDNA tag pyrosequencing to investigate the associated prokaryotic diversity. Dense assemblages of prokaryotes were associated with the hydrant surface. Two microbial morphotypes were observed: one horseshoe-shaped and one fusiform, worm-like. These prokaryotes were observed on the hydrozoan epidermis, but not in the portions covered by the perisarcal exoskeleton, and their abundance was higher in March while decreased in late spring. Molecular analyses showed that assemblages were dominated by Bacteria rather than Archaea. Bacterial assemblages were highly diversified, with up to 113 genera and 570 Operational Taxonomic Units (OTUs), many of which were rare and contributed to <0.4%. The two most abundant OTUs, likely corresponding to the two morphotypes present on the epidermis, were distantly related to Comamonadaceae (genus Delftia) and to Flavobacteriaceae (genus Polaribacter). Epibiontic bacteria were found on E. crocea from different geographic areas but not in other hydroid species in the same areas, suggesting that the host-microbe association is species-specific. This is the first detailed report of bacteria living on the hydrozoan epidermis, and indeed the first study reporting bacteria associated with the epithelium of E. crocea. Our results provide a starting point for future studies aiming at clarifying the role of this peculiar hydrozoan-bacterial association

The Role of Transporters in the Pharmacokinetics of Orally Administered Drugs

Drug transporters are recognized as key players in the processes of drug absorption, distribution, metabolism, and elimination. The localization of uptake and efflux transporters in organs responsible for drug biotransformation and excretion gives transporter proteins a unique gatekeeper function in controlling drug access to metabolizing enzymes and excretory pathways. This review seeks to discuss the influence intestinal and hepatic drug transporters have on pharmacokinetic parameters, including bioavailability, exposure, clearance, volume of distribution, and half-life, for orally dosed drugs. This review also describes in detail the Biopharmaceutics Drug Disposition Classification System (BDDCS) and explains how many of the effects drug transporters exert on oral drug pharmacokinetic parameters can be predicted by this classification scheme

Effectiveness of the Brazilian influenza vaccination policy, a systematic review Efetividade da política brasileira de vacinação contra influenza, uma revisão sistemática

Since 1999, Brazil has undertaken annual influenza vaccine campaigns, free of charge, targeting the elderly population, health professionals, and immune-deficient patients. We conducted a systematic review of literature in order to evaluate the effectiveness of the initiative. We used the keywords influenza, vaccine, Brazil and effectiveness to search the main databases. Thirty-one studies matched our inclusion and exclusion criteria. Influenza vaccine coverage among the elderly is high, though not as high as suggested by the official figures. Estimates on effectiveness are scarce. The majority come from ecological studies that show a modest reduction in mortality and hospital admissions due to influenza-related causes. Such reduction is not evident in the North and Northeastern states of Brazil, a finding that is probably related to the different seasonal pattern of influenza in equatorial and tropical regions. Brazilian epidemiologists still owe society better-designed studies addressing the effectiveness of influenza vaccine campaigns.<br>Desde 1999 o Brasil realiza campanhas anuais de vacinação contra influenza. A vacinação é gratuita, e tem como grupos alvo os idosos, os profissionais de saúde e os pacientes com comprometimento imunitário. Nós realizamos uma revisão sistemática da literatura, com o propósito de avaliar a efetividade desta iniciativa. Foram usadas as palavras chave influenza, vacina, Brasil e efetividade, para a busca nas bases de dados mais relevantes. Trinta e um estudos se encaixaram nos nossos critérios de inclusão e exclusão. A cobertura vacinal nos idosos é alta, porém não tão alta como apresentado nos dados oficiais. Estimativas de efetividade são escassas. A maioria delas vem de estudos ecológicos, que demonstram um modesto efeito na redução da mortalidade e internações hospitalares por causas relacionadas à influenza. Esta redução não se evidencia nos estados do Norte e Nordeste do Brasil, achado que provavelmente relaciona-se ao padrão distinto de sazonalidade da influenza nas regiões equatoriais e tropicais. Os epidemiologistas brasileiros ainda devem à sociedade estudos com desenhos mais adequados à análise da efetividade das campanhas de vacinação contra influenza

Integrated weed management in rice

Weeds are one of the major constraints in rice production systems. The development and adoption of proper integrated weed management strategies must form an integral part of sustainable rice production. An integrated weed management is defined as combinations of two or more weed control measures at low inputs in order to reduce weed competition in rice below an economical threshold level. Use of preventive methods and cultural practices such as crop simulation through nutrient application, method of rice sowing, planting time, plant population, and weed competitive cultivars have been recommended for minimizing impact of weeds on crop yield. The use of herbicide rotation, herbicide-resistant crops, bioherbicides, and their integration with hand tools or agricultural implements should be encouraged for better weed control efficiency