129 research outputs found

    Floristic and forest inventory of Santa Catarina: species of evergreen rainforest.

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    O presente trabalho objetivou apresentar a lista de espécies da floresta pluvial subtropical (Floresta Ombrófila Densa) em Santa Catarina, com base em 202 unidades amostrais implantadas pelo Inventário Florístico Florestal de Santa Catarina para estudo do componente arbóreo-arbustivo e da regeneração, além de coletas florísticas externas às unidades amostrais. Foram registradas 1.473espécies, 19,0% das espécies citadas para esta tipologia florestal no Brasil, dentre estas três gimnospermas e 1.470 angiospermas. As famílias mais ricas em espécies foram: Orchidaceae (143 espécies), Myrtaceae (142), Asteraceae (98), Melastomataceae (86), Fabaceae (78), Rubiaceae (65), Solanaceae (61), Bromeliaceae (57), Piperaceae (56) e Lauraceae (52). Entre as espécies registradas, oito constam na Lista Oficial das Espécies da Flora Brasileira Ameaçadas de Extinção: Aechmea blumenavii, Araucaria angustifolia, Billbergia alfonsijoannis, Euterpe edulis, Heliconia farinosa, Ocotea catharinensis, O. odorifera e O. porosa

    The Telomerase Database

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    Telomerase is a ribonucleoprotein enzyme that extends DNA at the chromosome ends in most eukaryotes. Since 1985, telomerase has been studied intensively and components of the telomerase complex have been identified from over 160 eukaryotic species. In the last two decades, there has been a growing interest in studying telomerase owing to its vital role in chromosome stability and cellular immortality. To keep up with the remarkable explosion of knowledge about telomerase, we compiled information related to telomerase in an exhaustive database called the Telomerase Database (http://telomerase.asu.edu/). The Telomerase Database provides comprehensive information about (i) sequences of the RNA and protein subunits of telomerase, (ii) sequence alignments based on the phylogenetic relationship and structure, (iii) secondary structures of the RNA component and tertiary structures of various subunits of telomerase, (iv) mutations of telomerase components found in human patients and (v) active researchers who contributed to the wealth of current knowledge on telomerase. The information is hierarchically organized by the components, i.e. the telomerase reverse transcriptase (TERT), telomerase RNA (TR) and other telomerase-associated proteins. The Telomerase Database is a useful resource especially for researchers who are interested in investigating the structure, function, evolution and medical relevance of the telomerase enzyme

    Inventário Florístico Florestal de Santa Catarina: espécies da Floresta Estacional Decidual.

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    O presente trabalho visou apresentar e analisar a flora da Floresta Estacional Decidual em Santa Catarina, Brasil, tendo como fonte de dados os levantamentos realizados durante o Inventário Florístico Florestal de Santa Catarina. Foram avaliadas as espécies de indivíduos lenhosos de 79 unidades amostrais de 4.000 m². Foram feitas coletas extras de indivíduos férteis, no entorno e nas unidades amostrais, das demais formas de vida. Este esforço amostral registrou 420 espécies, abrangendo 90 famílias e 275 gêneros. Nas unidades amostrais, registrou-se 233 espécies, sendo 204 com diâmetro na altura do peito (DAP) ? 10 cm e 162 com diâmetro na altura do peito DAP ? 10 cm e altura ? 1,50 m, portanto com espécies em comuns. A coleta de material extra registrou 332 angiospermas e uma gimnosperma (Araucaria angustifolia), demonstrando a importância de coletas externas às áreas previamente delimitadas. Entre as ameaçadas de extinção foram registradas Ocotea odorifera e Araucaria angustifolia

    Inventário de Dicksonia sellowiana Hook. em Santa Catarina.

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    (Inventário de Dicksonia sellowiana Hook. em Santa Catarina). A intensa exploração comercial de Dicksonia sellowiana a deixaram na categoria de ameaçada de extinção, sendo incluída na lista brasileira de espécies da flora ameaçada de extinção. Por isso, o presente trabalho visa apresentar o estudo da distribuição das populações de D. sellowiana para apontar os locais de maior ocorrência da espécie em Santa Catarina, e avaliar a relação da densidade populacional com fatores ambientais, como altitude e clima. Foram instaladas 225 unidades amostrais, com 4.000 m2 nas regiões do planalto e oeste catarinense, baseadas na grade de 10 km x 10 km conforme procedimento do Inventário Florístico Florestal de Santa Catarina. Nestas 225 unidades amostrais, D. sellowiana foi encontrada em 94, com variação de um a 391 indivíduos. A grande densidade da espécie em áreas mais elevadas (superiores a 1.000 m) está relacionada com os dados climáticos que influenciam a população diretamente, apontados pelo Critério de Informação de Akaike corrigido, ou seja, a altitude e a variação de temperatura que ela provoca. Observou-se também, que a espécie apresenta maior concentração da população nos menores intervalos de altura e diâmetro, chegando a elevadas densidades com até 977 indivíduos/ha em algumas áreas. As informações obtidas pelo inventário sobre esta espécie possibilitam indicar medidas de conservação para a espécie, como área a serem conservadas e apoio a produtores rurais

    Structure of mixed ombrophyllous forests with Araucaria angustifolia (Araucariaceae) under external stress in Southern Brazil.

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    This study is part of the Floristic and Forest Inventory of Santa Catarina, conceived to evaluate forest resources, species composition and structure of forest remnants, providing information to update forest conservation and land use policy in Southern Brazilian State of Santa Catarina (95 000km²). In accordance to the Brazilian National Forest Inventory (IFN-BR), the inventory applies systematic sampling, with 440 clusters containing four crosswise 1 000m² plots (20x50m) each, located on a 10x10km grid overlaid to land use map based on classification of SPOT-4 images from 2005. Within the sample units, all woody individuals of the main stratum (DBH?10cm) are measured and collected (fertile and sterile), if not undoubtedly identified in field. Regeneration stratum (height>1.50m; DBH<10cm) is registered in 100m² in each sample unit. Floristic sampling includes collection of all fertile trees, shrubs and herbs within the sample unit and in its surroundings. This study performs analysis based on 92 clusters measured in 2008 within an area of 32 320km² of mixed ombrophyllous forests with Araucaria angustifolia located at the state’s high plateau (500m to 1 560m above sea level at 26º00’-28º30’ S and 49º13’-51º23’ W). Mean density (DBH?10cm) is 578 individuals/ha (ranging from 85/ha to 1 310/ha), mean species richness in measured remnants is 35 (8 to 62), Shannon and Wiener diversity index (H’) varies between 1.05 and 3.48. Despite high total species diversity (364 Magnoliophyta, five Coniferophyta and one tree fern) and relatively high mean basal area (25.75m²/ha, varying from 3.87 to 68.85m²/ ha), the overwhelming majority of forest fragments are considered highly impacted and impoverished, mostly by logging, burning and extensive cattle farming, turning necessary more efficient protection measures. Basal area was considered an appropriate indicator for stand quality and conservation status

    Endonuclease-independent LINE-1 retrotransposition at mammalian telomeres

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    Long interspersed element-1 (LINE-1 or L1) elements are abundant, non-long-terminal-repeat (non-LTR) retrotransposons that comprise 17% of human DNA(1). The average human genome contains similar to 80-100 retrotransposition- competent L1s (ref. 2), and they mobilize by a process that uses both the L1 endonuclease and reverse transcriptase, termed target-site primed reverse transcription(3-5). We have previously reported an efficient, endonuclease-independent L1 retrotransposition pathway (ENi) in certain Chinese hamster ovary (CHO) cell lines that are defective in the non-homologous end-joining (NHEJ) pathway of DNA double-strand-break repair(6). Here we have characterized ENi retrotransposition events generated in V3 CHO cells, which are deficient in DNA-dependent protein kinase catalytic subunit (DNA-PKcs) activity and have both dysfunctional telomeres and an NHEJ defect. Notably, similar to 30% of ENi retrotransposition events insert in an orientation-specific manner adjacent to a perfect telomere repeat (5'-TTAGGG-3'). Similar insertions were not detected among ENi retrotransposition events generated in controls or in XR-1 CHO cells deficient for XRCC4, an NHEJ factor that is required for DNA ligation but has no known function in telomere maintenance. Furthermore, transient expression of a dominant-negative allele of human TRF2 ( also called TERF2) in XRCC4-deficient XR-1 cells, which disrupts telomere capping, enables telomere-associated ENi retrotransposition events. These data indicate that L1s containing a disabled endonuclease can use dysfunctional telomeres as an integration substrate. The findings highlight similarities between the mechanism of ENi retrotransposition and the action of telomerase, because both processes can use a 3' OH for priming reverse transcription at either internal DNA lesions or chromosome ends(7,8). Thus, we propose that ENi retrotransposition is an ancestral mechanism of RNA-mediated DNA repair associated with non-LTR retrotransposons that may have been used before the acquisition of an endonuclease domain.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62964/1/nature05560.pd
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