Transitions Regulating the Timing of Cytokinesis in Embryonic Cells

AbstractAnaphase, mitotic exit, and cytokinesis proceed in rapid succession, and while mitotic exit is a requirement for cytokinesis in yeast [1, 2], it may not be a direct requirement for furrow initiation in animal cells [3, 4]. In this report, we physically manipulated the proximity of the mitotic apparatus (MA) to the cell cortex in combination with microinjection of effectors of the spindle checkpoint and CDK1 activity to determine how the initiation of cytokinesis is coupled to the onset of anaphase and mitotic exit. Whereas precocious contact between the MA and the cell surface advanced the onset of cytokinesis into early anaphase A, furrowing could not be advanced prior to the metaphase-anaphase transition. Additionally, while cells arrested in anaphase could be induced to initiate cleavage furrows, cells arrested in metaphase could not. Finally, activation of the mitotic checkpoint in one spindle of a binucleate cell failed to arrest cytokinesis induced by the control spindle but did inhibit the formation of furrows between the arrested MA and the control, nonarrested MA. Our experiments suggest that the competence of the mitotic apparatus to initiate cytokinesis is not dependent on cyclin degradation but does require anaphase-promoting complex (APC) activity and, thus, inactivation of the mitotic checkpoint

Distributed Adaptive Attitude Synchronization of Multiple Spacecraft

This paper addresses the distributed attitude synchronization problem of multiple spacecraft with unknown inertia matrices. Two distributed adaptive controllers are proposed for the cases with and without a virtual leader to which a time-varying reference attitude is assigned. The first controller achieves attitude synchronization for a group of spacecraft with a leaderless communication topology having a directed spanning tree. The second controller guarantees that all spacecraft track the reference attitude if the virtual leader has a directed path to all other spacecraft. Simulation examples are presented to illustrate the effectiveness of the results.Comment: 13 pages, 11 figures. To appear in SCIENCE CHINA Technological Science

Escherichia coli induces apoptosis and proliferation of mammary cells

Mammary cell apoptosis and proliferation were assessed after injection of Escherichia coli into the left mammary quarters of six cows. Bacteriological analysis of foremilk samples revealed coliform infection in the injected quarters of four cows. Milk somatic cell counts increased in these quarters and peaked at 24 h after bacterial injection. Body temperature also increased, peaking at 12 h postinjection, The number of apoptotic cells was significantly higher in the mastitic tissue than in the uninfected control. Expression of Bax and interleukin-1 beta converting enzyme increased in the mastitic tissue at 24 h and 72 h postinfection, whereas Bcl-2 expression decreased at 24 h but did not differ significantly from the control at 72 h postinfection, Induction of matrix metalloproteinase-g, stromelysin-1 and urokinase-type plasminogen activator was also observed in the mastitic tissue. Moreover, cell proliferation increased in the infected tissue, These results demonstrate that Escherichia coli-induced mastitis promotes apoptosis and cell proliferation

Paleocene to Pliocene low-latitude, high-elevation basins of southern Tibet: implications for tectonic models of India-Asia collision, Cenozoic climate, and geochemical weathering

The elevation history of the Tibetan Plateau promises insight into the mechanisms and dynamics that develop and sustain high topography over tens of millions of years. We present the first nearly continuous Cenozoic elevation history from two sedimentary basins on the southern Tibetan Plateau within the latest Cretaceous to Eocene Gangdese arc. Oxygen-isotope and Δ47 clumped-isotope compositions of nonmarine carbonates allow us to constrain carbonate formation temperature and reconstruct the paleoprecipitation record of the Eocene to Pliocene Oiyug Basin and Paleocene to Eocene Penbo Basin. We exploit the systematic decrease of surface temperature and meteoric water δ18O values with elevation to derive paleoelevation estimates for these basins. Minimally altered and unaltered pedogenic and lacustrine carbonates from the Oiyug Basin yield Δ47, CDES (relative to the carbon dioxide equilibrium scale [CDES]) values of 0.625‰ to 0.755‰, which correspond to temperatures of 1−30 °C using a Δ47 thermometer for low-temperature carbonates. Similarly, the Penbo Basin yielded Δ47, CDES values of 0.701‰ to 0.726‰, corresponding to temperatures of 6−12 °C. The apparent evidence for survival of primary clumped-isotope values in the face of substantial burial and heating is an important result for the field of carbonate clumped-isotope thermometry. Our paleoelevation estimates for the Eocene to Pliocene Oiyug Basin (∼6.5−4.1 km) support previous evidence that high elevations were attained in southern Tibet by at least ca. 30 Ma. Stable-isotope results allow for the possibility of significant topographic subsidence during the Miocene as a result of regional extension. In the Penbo Basin, our paleoelevation estimates for the Paleocene to Eocene Nianbo Formation (4.4 +1.3/−1.7 km) and Eocene Pana Formation (4.1 +1.2/−1.6 km) extend the altitude record of the southern Tibetan Plateau to pre−India-Asia collision. These results support the “Lhasaplano” model of an Andean-type continental margin tectonic system. The rise of the Himalayas and Tibet is often invoked to understand isotopic proxies for global chemical weathering in the Cenozoic and has constrained the debate on the nature of CO2−climate−weathering feedbacks. The nature of the Tibetan paleoelevations from pre- to postcollision, as presented here, indicates that high relief at low latitude prevailed on the Asian margin much earlier than previously thought. Thus, high topography alone at low latitude is not sufficient to account for the Cenozoic weathering proxy record

Turbulence properties and kinetic signatures of electron in Kelvin-Helmholtz waves during a geomagnetic storm

We present a comprehensive study of Magnetospheric Multiscale (MMS) spacecraft encounter with KHI during a geomagnetic storm, focusing on elucidating key turbulence properties and reconnection signatures observed at the edges of KH vortices. The spectral slope for electric field stays approximately constant for frequencies below the ion cyclotron frequency and exhibits a break around the lower hybrid frequency, indicating wave activity. Furthermore, MMS observes a current sheet accompanied by intense electron jets and features consistent with strong guide-field asymmetric reconnection across the magnetopause. Substantial agyrotropy (by a factor of 10) in electron distribution functions is observed in the reconnecting current sheet and at the edges of KH. Our observation presents a multi-scale view into KH turbulence under strongly driven conditions and into the dynamics occurring at electron dissipation scales.Comment: 10 pages, including 4 figure

Emergence of Skyrme crystal in Gross-Neveu and 't Hooft models at finite density

We study two-dimensional, large $N$ field theoretic models (Gross-Neveu model, 't Hooft model) at finite baryon density near the chiral limit. The same mechanism which leads to massless baryons in these models induces a breakdown of translational invariance at any finite density. In the chiral limit baryonic matter is characterized by a spatially varying chiral angle with a wave number depending only on the density. For small bare quark masses a sine-Gordon kink chain is obtained which may be regarded as simplest realization of the Skyrme crystal for nuclear matter. Characteristic differences between confining and non-confining models are pointed out.Comment: 27 pages, 11 figures, added reference, corrected sig

SU(7) Unification of SU(3)_C*SU(4)_W* U(1)_{B-L}

We propose the SUSY SU(7) unification of the SU(3)_C* SU(4)_W* U(1)_{B-L} model. Such unification scenario has rich symmetry breaking chains in a five-dimensional orbifold. We study in detail the SUSY SU(7) symmetry breaking into SU(3)_C* SU(4)_W* U(1)_{B-L} by boundary conditions in a Randall-Sundrum background and its AdS/CFT interpretation. We find that successful gauge coupling unification can be achieved in our scenario. Gauge unification favors low left-right and unification scales with tree-level \sin^2\theta_W=0.15. We use the AdS/CFT dual of the conformal supersymmetry breaking scenario to break the remaining N=1 supersymmetry. We employ AdS/CFT to reproduce the NSVZ formula and obtain the structure of the Seiberg duality in the strong coupling region for 3/2N_c<N_F<3N_C. We show that supersymmetry is indeed broken in the conformal supersymmetry breaking scenario with a vanishing singlet vacuum expectation value.Comment: 25 pages, 1 figure

The VocalNotes Dataset

The VocalNotes dataset is a collection of audio and annotations for excerpts of vocal performances from five musical traditions - Japanese Minyo, Chinese Hebei Bangzi opera, Russian traditional singing, Alpine yodel and Jewish Romaniote chant. For each tradition the dataset contains: about 10 minutes of audio; documentation for the songs from which annotated fragments originate; f0, independent onset, offset and note pitch annotations created by two or three experts; The dataset was created as part of the VocalNotes project [1]. It is released under CC-BY-NC-SA license and can be accessed by filling out a request form

Field-Aligned Current Structures during the Terrestrial Magnetosphere's Transformation into Alfven Wings and Recovery

On April 24th, 2023, a CME event caused the solar wind to become sub-Alfvenic, leading to the development of an Alfven Wing configuration in the Earth's Magnetosphere. Alfven Wings have previously been observed as cavities of low flow in Jupiter's magnetosphere, but the observing satellites did not have the ability to directly measure the Alfven Wings' current structures. Through in situ measurements made by the Magnetospheric Multiscale (MMS) spacecraft, the April 24th event provides us with the first direct measurements of current structures during an Alfven Wing configuration. We have found two distinct types of current structures associated with the Alfven Wing transformation as well as the magnetosphere recovery. These structures are observed to be significantly more anti-field-aligned and electron-driven than typical magnetopause currents, indicating the disruptions caused to the magnetosphere current system by the Alfven Wing formation