Heterotopic heart transplantation in the rat: a preliminary study.

The aims of this study are to settle and verify how trustful is the heterotopic transplantation of the heart in an experimental model utilizing the original technique of Abbott modified by Ono and Lindsey distinguishing from it for the administration of the anaesthesia and the preservation of the organ. In this type of operation is necessary to realize only two micro-surgical vascular anastomosis that represent the basis of any other micro-surgical operation of organ's transplantation in the rat. There have been used 30 rats of the Wistar stock of male sex and weighing about 200-300 grs. both as donor and as receiving. The anaesthesia consisted in the administration of methoxyflourane by inhalation during the operation. The heart is kept in a container with Stanford solution on ice. The 77% of the cases (23 on 30) have been successful, even considering the ischaemic damage that has been evaluated examining the cardiac beat and the color of the organ. The study of Ono and Lindsey gave as result the success of the operation on 91% of cases (147 on 161 operated), compared to the result obtained by us, on a smaller number of cases (77% of success on 30 operated rats) permits to consider this experimental model to be utilizable even for further more complex studies

PRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICO

The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in milk production as well as milk in fat production. The immunological diagnosis involves purchasing costly diagnostic kits on the market. Therefore, the aim of this study was the production of hybridomas secreting polyclonal antibodies with affinity to Neospora caninum (Nc-1 strain) for immunodiagnostic use. For antibodies production, we used sonicated protozoa from Vero cells in culture, purified by filtration. These tachyzoites were employed for immunization of BALB / c mice using saponin as adjuvant, which allowed obtaining polyclonal antibodies capable of revealing fluorescein reaction in indirect immunofluorescence. The fusion of splenic cells, from the immunized mice with myeloma cells SP2 / 0 resulted in 72.4% hybridomas secreting anti-Nc-1antibodies. These hybridomas secreted antibodies positive to N. caninum and negative to Toxoplasma gondii