16,630 research outputs found
Clonal variation in cell surface display of an H-2 protein lacking a cytoplasmic tail
Truncated variants of the gene encoding H-2Ld, an integral membrane protein encoded by the major histocompatibility complex, were constructed by in vitro mutagenesis to elucidate the function of charged amino acids found on the cytoplasmic side of the transmembrane (TM) region. Analysis of cloned L cells transfected with these genes shows that the seven amino acids following the TM segment, four of which are basic, enhance the cell surface expression of H-2Ld protein but are not required for it. However, some clones do not express a tailless H-2Ld protein on the cell surface but express it intracellularly where it has a long half-life. Turnover measurements on cell surface H-2Ld proteins suggest that the basic residues following the TM segment are not a "stop transfer" sequence (Blobel, G., 1980, Proc. Natl. Acad. Sci. USA., 77:1496-1500) which anchors the H-2Ld protein in the membrane. Pulse-chase and endoglycosidase H sensitivity studies show that H-2Ld proteins lacking some or all of the basic residues and H-2Ld proteins which have a full-length cytoplasmic tail are processed with different kinetics. These results suggest an involvement of the membrane-proximal region of the cytoplasmic tail in the intracellular transport of H-2Ld. We further suggest that the L cell clones which do and do not express a tailless H-2Ld protein on the cell surface differ in the ability to transport a tailless integral membrane protein to the cell surface
Complete hyperfine Paschen-Back regime at relatively small magnetic fields realized in Potassium nano-cell
A one-dimensional nano-metric-thin cell (NC) filled with potassium metal has
been built and used to study optical atomic transitions in external magnetic
fields. These studies benefit from the remarkable features of the NC allowing
one to use - and -methods for effective investigations of
individual transitions of the K D_1 line. The methods are based on strong
narrowing of the absorption spectrum of the atomic column of thickness L equal
to and to (with \lambda = 770\un{nm} being the resonant
laser radiation wavelength). In particular, for a -polarized radiation
excitation the -method allows us to resolve eight atomic transitions
(in two groups of four atomic transitions) and to reveal two remarkable
transitions that we call Guiding Transitions (GT). The probabilities of all
other transitions inside the group (as well as the frequency slope versus
magnetic field) tend to the probability and to the slope of GT. Note that for
circular polarization there is one group of four transitions and GT do not
exist. Among eight transitions there are also two transitions (forbidden for
= 0) with the probabilities undergoing strong modification under the
influence of magnetic fields. Practically the complete hyperfine Paschen-Back
regime is observed at relatively low (\sim 1\un{kG}) magnetic fields. Note
that for K line GT are absent. Theoretical models describe the experiment
very well.Comment: 6 page
Expansions of the solutions of the biconfluent Heun equation in terms of incomplete Beta and Gamma functions
Starting from equations obeyed by functions involving the first or the second
derivatives of the biconfluent Heun function, we construct two expansions of
the solutions of the biconfluent Heun equation in terms of incomplete Beta
functions. The first series applies single Beta functions as expansion
functions, while the second one involves a combination of two Beta functions.
The coefficients of expansions obey four- and five-term recurrence relations,
respectively. It is shown that the proposed technique is potent to produce
series solutions in terms of other special functions. Two examples of such
expansions in terms of the incomplete Gamma functions are presente
Stimulated Raman Adiabatic Passage via bright state in Lambda medium of unequal oscillator strengths
We consider the population transfer process in a Lambda-type atomic medium of
unequal oscillator strengths by stimulated Raman adiabatic passage via
bright-state (b-STIRAP) taking into account propagation effects. Using both
analytic and numerical methods we show that the population transfer efficiency
is sensitive to the ratio q_p/q_s of the transition oscillator strengths. We
find that the case q_p>q_s is more detrimental for population transfer process
as compared to the case where . For this case it is possible to
increase medium dimensions while permitting efficient population transfer. A
criterion determining the interaction adiabaticity in the course of propagation
process is found. We also show that the mixing parameter characterizing the
population transfer propagates superluminally
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