Efeito de diferentes meios de cultura e da luz no crescimento e na esporulação de Verticillium lecanii.

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso

Efeito de diferentes regimes de luminosidade na produção de Verticillium lecanii (Zimm.) Viégas em meio sólido.

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso

Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil.

The use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates. This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil. The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil. The bacteria were authenticated and evaluated regarding to their symbiotic capacity. The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box- (PCR). Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures. For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted. The majority of the obtained isolates showed rapid growth and acidified the culture medium. Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates. The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut

Mancha-foliar-de-Phaeosphaeria (mancha-branca-do-milho): fungo ou bactéria?

Diferentes espécies de fungos e a bactéria Pantoea ananatis (Pa) foram relatados como agentes etiológicos da doença mancha-de-Phaeosphaeria (MF) do milho. Este trabalho teve como objetivo a identificação molecular de fungos e bactérias que ocorrem em MF e sua etiologia. Os DNAs genômicos de 1) amostras de cada um dos quatro estágios das lesões MF; 2 ) bactérias e fungos isolados de lesões naturais (NPLS) e lesões artificial (AI), e 3) fungos isolados de lesões obtidas a partir de plantas inoculadas com Pa em casa de vegetação (GH) foram utilizados em reações de PCR com primers universais para genes rRNA de bactérias e fungos, e primers espécie específicos para Pa. Amplicons bacterianos foram observados em todas as fases das lesões e amplicons fúngicos foram observados nos estádios 3 e 4. Amplicons bacterianos de amostras NPLS eram de Pa enquanto amplicons fúngicos foram de Phaeosphaeria sp. e Phoma sp. Bactérias nas NPLS, GH e lesões AI foram identificadas como Pa, Pa e Bacillus subtilis, respectivamente, enquanto os fungos foram Epicoccum nigrum, Leptosphaeria sacchari, Cochliobolus geniculatus, Pithomyces chartarum, Alternaria alternata, A. ricini, Gibberella intricans, G. fujikuroi, Phaeosphaeria sp. , P. avenaria, Phoma sp., Phyllosticta sp., Sarocladium strictum, Glomerella graminicola e Cochliobolus heterostrophus. Os resultados indicam fortemente que Pa é o agente causador da doença PLS do milho e que diferentes espécies de fungos oportunistas só aparecem no final das fases de necrose de lesões causadas por Pa.bitstream/item/97961/1/bol-79.pd

IFCARDS: AVALIAÇÃO DE ACEITAÇÃO DE TECNOLOGIA POR ALUNOS DO ENSINO MÉDIO DO IFS

Forgetfulness is a natural and gradual process of the humanbrain. He is responsible for loss of memory informations notreviewed periodically. At graduate courses, the students attendnumerous curricular units where several subjects aretaught. However, without review, they are forgotten, affectingperformance in assessments such as ENADE. Thus,this work presents a multiplatform application for spacedcontent review with SM2 algorithm through mini-games. Itwas evaluated quantitatively using the Technology AcceptanceModel (TAM) using the ve level Likert scale by 35users. The results show that acceptance was greater than78 % in all categories, with a recommendation for use by88.6 % of respondents. In the qualitative assessment, wordclouds were used, which presented a positive scenario in thelearning process with 57.89 % of students satised with theIFCards