Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features

Imaging of mesophyll cell suspensions prepared from Arabidopsis has been pivotal for forming our current understanding of the molecular control of chloroplast division over the past 25 years. In this chapter, we provide a method for the preparation of leaf cell suspensions that improves upon a previous method by optimizing cellular preservation and cell separation. This technique is accessible to all researchers and amenable for use with all plant species. The leaf suspensions can be used for imaging chloroplast features within a cell that are important for photosynthesis such as size, number, and distribution. However, we also provide examples to illustrate how the cells in the suspensions can be easily stained to image other features, for example pit fields where plasmodesmata are located and organelles such as mitochondria, to improve our understanding of traits that are important for photosynthetic physiology

Engaging Teenagers with Science Through Comics

It is increasingly important for all citizens, and especially youth, to understand how viruses impact our health, communities and environment. Particularly for youth less interested in traditional science learning materials, comics may provide a way to engage teenagers with scientific information about viruses. We compared the impacts of different formats of educational materials on teenagers’ knowledge of, attitudes toward, and engagement with information about viruses. High school students (N = 873) were randomly assigned to read either a high quality comic or essay about viruses. Latent class analysis grouped youth into one of four categories that captured a range from low to high science identity. We compared material type (comic/essay) and science identity (low to high) on knowledge, attitudes and engagement. There were no comic/essay differences on knowledge, nor on attitudes about the importance of or interest in viruses. Across all levels of science identity, however, teenagers in the comic group were significantly more likely to want to read more similar materials than teenagers in the essay group, thus indicating more engagement. This effect was more pronounced among youth in the low compared to the higher identity categories. Our findings support the notion that comics can appeal to a wider audience of learners than traditional essays while still resulting in similar knowledge scores. This suggests that comics can be an important and effective tool to engage a broad spectrum of youth with science learning materials