A novel dry method for surface modification of SU-8 for immobilization of biomolecules in Bio-MEMS

SU-8 has been primarily used for structural elements and microfludics components in MEMS. Microsystems for biological applications require immobilization of biomolecules on the MEMS structures. In order to functionalize SU-8 for such purposes, the surface needs to be modified. In this paper, we report a novel dry method of surface modification of SU-8 which is compatible with standard microfabrication techniques. The surface obtained by spin coating SU-8 (2002) on silicon wafer was modified by grafting amine groups using pyrolytic dissociation of ammonia in a hotwire CVD setup. To demonstrate the presence of amine groups on modified SU-8 surface, the surface characteristic after modification was assessed using Fourier transform infrared spectroscopy. The change in SU-8 surface morphology before and after surface modification was investigated using atomic force microscopy. To show the utility of this process for application in Bio-MEMS, SU-8 microcantilevers were fabricated and subjected to the same surface modification protocol. Following this, the cantilevers were incubated first in a suspension of human immunoglobulin (HIgG) and then in FITC tagged goat anti-human IgG in order to demonstrate the utility of the surface modification performed. The efficacy of the process was assessed by observing the cantilevers under a fluorescence microscope

Influence of different organic sources of nutrients on growth and flowering behaviour of pomegranate (Punica granatum L.) cv. Bhagwa

A field experiment on the influence of different organic sources of nutrients on growth and flowering behaviour of pomegranate (Punica granatum L.) cv. Bhagwa was conducted at College Farm, College of Horticulture, Sardarkrushinagar Dantiwada Agricultural University, Jagudan, District-Mehsana in Gujarat, India during Mrig bahar (June-January)  2017-18 and 2018-19. The present investigation was laid out in Randomized Block Design with three replications consisting of twenty-two treatments with four different organic manures viz., farmyard manure, vermicompost, poultry manure and neem cake as a source of nitrogen and recommended dose of manure and fertilizers based on plant age with or without biofertilizers (Azotobacter, PSB & KMB) and biopesticides (Trichoderma viride and Paecilomyces lilacinus). The results based on pooled data revealed that a maximum number of hermaphrodite flowers (85.17) and incomplete flowers (96.50) up to two months after treatment application, fruit set (69.45 %) along with minimum fruit drop (13.18 %) and days taken for marketable picking (168.83) were significantly (@5%) noted under treatment 100 % RDN through poultry manure + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. However, significantly (@5%) highest incremental primary growth parameters viz., plant height (0.60 m), plant spread (E-W: 0.41 m and N-S: 0.43 m) and stem girth (1.47 cm) after two months of treatment application were observed under treatment 100 % RDN through vermicompost + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. Thus, the organic sources viz., vermicompost and poultry manure with biofertilizers and biopesticides would be very helpful for enhancing vegetative growth and flowering behaviour of the pomegranate

Downregulation of host tryptophan-aspartate containing coat (TACO) gene restricts the entry and survival of Leishmania donovani in human macrophage model

Leishmania are obligate intracellular protozoan parasites of mammalian hosts. Promastigotes of Leishmania are internalized by macrophages and transformed into amastigotes in phagosomes, and replicate in phagolysosomes. Phagosomal maturation arrest is known to play a crucial role in the survival of pathogenic Leishmania within activated macrophages. Recently, tryptophan-aspartate containing coat (TACO) gene has been recognized as playing a central role in the survival of Mycobacterium tuberculosis within human macrophages by arresting the phagosome maturation process. We postulated that a similar association of TACO gene with phagosomes would prevent the vacuole from maturation in the case of Leishmania. In this study we attempted to define the effect of TACO gene downregulation on the entry/survival of Leishmania donovani intracellularly, by treatment with Vitamin D3 (Vit.D3)/Retinoic acid (RA) and chenodeoxycholic acid (CDCA)/RA combinations in human THP-1 macrophages (in vitro). Treatment with these molecules downregulated the TACO gene in macrophages, resulting in reduced parasite load and marked reduction of disease progression in L. donovani infected macrophages. Taken together, these results suggest that TACO gene downregulation may play a role in subverting macrophage machinery in establishing the L. donovani replicative niche inside the host. Our study is the first to highlight the important role of the TACO gene in Leishmania entry, survival and to identify TACO gene downregulation as potential drug target against leishmaniasis

Comparison of Recovery Capital in Patients with Alcohol and Opioid Dependence – An Exploratory Study

Background: Recovery capital helps in the assessment of the personal strengths and challenges that exist in an individual with substance use which may have an impact on recovery process. This study aims at finding out the factors which help such individuals to sustain their recovery and how these factors differ across the two groups of people suffering from Alcohol Dependence Syndrome and Opioid Dependence Syndrome.Methods: A cross-sectional observational was designed where sociodemographic and clinical variables, the recovery capital ARC (Assessment of Recovery Capital) Scale and Severity of substance use SDS (Severity of Dependence) Scale of patients diagnosed with Alcohol Dependence Syndrome (ADS group) and those with Opioid Dependence Syndrome (ODS group) were assessed among patients not reporting withdrawal symptoms.Findings: A total of 49 subjects in the ODS group and 30 subjects in the ADS group were enrolled. The majority of the subjects in both groups were married, belonged to urban areas, practiced Hinduism, and were living in nuclear families. There was a significant difference between the educational status (p<0.001), religion practiced (p<0.001), age of onset of dependence (p<0.001), severity of dependence (p=0.11), and duration of abstinence (p<0.001) between the ADS and ODS groups. The mean scores on ARC Scale were 45.9 (S.D. =3.5) in the ODS group and 47.4 (S.D. =4.3) in the ADS group. ADS group had higher scores in Social Support Domain (p=0.034) and Housing and Safety domain (p=0.025). Other domains like global health, citizenship, meaningful activities, risk-taking, coping, and recovery experience did not significantly differ between the groups.Conclusion: This study aims at comparing the recovery capital of ADS patients with ODS patients. It also suggests that tailored treatment plans for people with ADS and ODS especially in housing and social support and common treatment approach in other domains of recovery will help them sustain the state for a longer term

Microstructural and mechanical properties of CNT-reinforced ZrO2-Y2O3 coated boiler tube steel T-91

The main purpose of this study was to fabricate carbon nanotubes (CNTs) reinforced zircon­nium yttrium coatings on boiler tube steel and to investigate the microstructural and mechanical properties of these coatings. Plasma sprayed conventional ZrO2-Y2O3, ZrO2-Y2O3 and 1 wt.% CNT and ZrO2-Y2O3 and 4 wt.% CNT were prepared and deposited successfully on boiler tube steel material T-91 (ASTM SA-213) by plasma thermal spray technology. Microhardness, porosity, XRD (X-ray diffraction), SEM/EDAX (scanning elec­tron microscopy/energy dispersive X-ray spectroscopy), X-ray mapping and cross-sectional analyses were used to analyse the specimens. The hardness of CNT reinforced ZrO2-Y2O3 increased with the increase in the percentage of CNT, whereas the porosity of the composite coatings decreased with the increase in the CNT percentage. The observed increase in hardness may be attributed to the content of CNT in the composite coating. The present research gives important information related to the fabrication and physical characteristics of CNT-reinforced ZrO2-Y2O3 coatings deposited on T-91 boiler tube steel