9 research outputs found
Intensifying adverbs in the English language
The article provides an analysis of various aspects of intensification in the English language (colloquial and newspaper), intensifying adverbs in particular. The processes of grammaticalisation and delexicalisation observed in the evolution of intensifying adverbs are described. The author also looks into the renewal and boundedness of intensifying adverbs, provides an overview of modern classifications of intensifying adverbs, and offers statistics on the frequency of occurrence of intensifying adverbs to illustrate their usage in spoken discourse and newspaper language. The findings assume that less intensification is used in written discourse whereas more intensification is employed in spoken discourse, which suggests that the decline in intensification is higher as the formality of the register increases, and vice versa
The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station
Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon's material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genus Mycobacteria, DNA of the strains of capsular bacteria Bacillus, and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genus Mycobacteria and the extreme bacterium of the genus Delftia in the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genus Mycobacteria was genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas' coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin. © 2018 T. V. Grebennikova et al
The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station
Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon's material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genus Mycobacteria, DNA of the strains of capsular bacteria Bacillus, and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genus Mycobacteria and the extreme bacterium of the genus Delftia in the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genus Mycobacteria was genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas' coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin. © 2018 T. V. Grebennikova et al