Synchrotron radiation photoionization mass spectrometry of laser ablated species

The present paper describes an experimental apparatus suitable to create and study free clusters by combining laser ablation and synchrotron radiation. First tests on sulfur samples, S, showed the production, through laser ablation, of neutral Sn clusters (n = 1–8). These clusters were ionized using synchrotron radiation at photon energies from 160 eV to 175 eV, across the S 2p core edge. The feasibility of such combined ablation–synchrotron radiation experiments is demonstrated, opening new possibilities on the investigation of free clusters and radical

Diversidad y variabilidad morfológica de catorce colectas de cacao (Theobroma cacao L.).

El cacao es un cultivo de gran importancia en el trópico americano, por lo tanto, el conocimiento y entendimiento de la diversidad genética de las colecciones colombianas de cacao contribuyen a la utilización y conservación de la especie, bien sea en programas de mejoramiento ó en el intercambbio del recurso germoplásmico. Con el propósito de proveer información acerca de la caracterización de los patrones de diversidad de la especie, se avaluaron 14 accesiones mediante 9 características morfológicas del fruto, de la colección Colombiana de cacao, localizada en el Centro de Investigación del ICA Palmira (Valle). En análisis de componentes principales mostró que los primeros 3 valores característicos explican el 83.8 por ciento de la variación total. El dendograma calculado a partir de la distancia de Mahalanobis entre pares de colectas indicó la existencia de alta variabilidad entre las accesiones y las clasificó en 3 gruposCacao-Theobroma caca

A cryptic transcription factor regulates Caulobacter adhesin development

Alphaproteobacteria commonly produce an adhesin that is anchored to the exterior of the envelope at one cell pole. In Caulobacter crescentus this adhesin, known as the holdfast, facilitates attachment to solid surfaces and cell partitioning to air-liquid interfaces. An ensemble of two-component signal transduction (TCS) proteins controls C. crescentus holdfast biogenesis by indirectly regulating expression of HfiA, a potent inhibitor of holdfast synthesis. We performed a genetic selection to discover direct hfiA regulators that function downstream of the adhesion TCS system and identified rtrC, a hypothetical gene. rtrC transcription is directly activated by the adhesion TCS regulator, SpdR. Though its primary structure bears no resemblance to any defined protein family, RtrC binds and regulates dozens of sites on the C. crescentus chromosome via a pseudo-palindromic sequence. Among these binding sites is the hfiA promoter, where RtrC functions to directly repress transcription and thereby activate holdfast development. Either RtrC or SpdR can directly activate transcription of a second hfiA repressor, rtrB. Thus, environmental regulation of hfiA transcription by the adhesion TCS system is subject to control by an OR-gated type I coherent feedforward loop; these regulatory motifs are known to buffer gene expression against fluctuations in regulating signals. We have further assessed the functional role of rtrC in holdfast-dependent processes, including surface adherence to a cellulosic substrate and formation of pellicle biofilms at air-liquid interfaces. Strains harboring insertional mutations in rtrC have a diminished adhesion profile in a competitive cheesecloth binding assay and a reduced capacity to colonize pellicle biofilms in select media conditions. Our results add to an emerging understanding of the regulatory topology and molecular components of a complex bacterial cell adhesion control system

Scalar cosmological perturbations from inflationary black holes

We study the correction to the scale invariant power spectrum of a scalar field on de Sitter space from small black holes that formed during a pre-inflationary matter dominated era. The formation probability of such black holes is estimated from primordial Gaussian density fluctuations. We determine the correction to the spectrum by first deriving the Keldysh propagator for a massless scalar field on Schwarzschild-de Sitter space. Our results suggest that the effect is strong enough to be tested -- and possibly even ruled out -- by observations.Comment: 41 pages, 11 figures, published versio

Physico-Chemical and structural interpretation of discrete derivative indices on N-tuples atoms

This report examines the interpretation of the Graph Derivative Indices (GDIs) from three different perspectives (i.e., in structural, steric and electronic terms). It is found that the individual vertex frequencies may be expressed in terms of the geometrical and electronic reactivity of the atoms and bonds, respectively. On the other hand, it is demonstrated that the GDIs are sensitive to progressive structural modifications in terms of: size, ramifications, electronic richness, conjugation effects and molecular symmetry. Moreover, it is observed that the GDIs quantify the interaction capacity among molecules and codify information on the activation entropy. A structure property relationship study reveals that there exists a direct correspondence between the individual frequencies of atoms and Hückel’s Free Valence, as well as between the atomic GDIs and the chemical shift in NMR, which collectively validates the theory that these indices codify steric and electronic information of the atoms in a molecule. Taking in consideration the regularity and coherence found in experiments performed with the GDIs, it is possible to say that GDIs possess plausible interpretation in structural and physicochemical terms. © 2016 by the authors; licensee MDPI, Basel, Switzerland.Pharmaceutical Preparation

The long and the short of it: Mechanisms of synchronous and compensatory dynamics across temporal scales

Synchronous dynamics (fluctuations that occur in unison) are universal phenomena with widespread implications for ecological stability. Synchronous dynamics can amplify the destabilizing effect of environmental variability on ecosystem functions such as productivity, whereas the inverse, compensatory dynamics, can stabilize function. Here we combine simulation and empirical analyses to elucidate mechanisms that underlie patterns of synchronous versus compensatory dynamics. In both simulated and empirical communities, we show that synchronous and compensatory dynamics are not mutually exclusive but instead can vary by timescale. Our simulations identify multiple mechanisms that can generate timescale-specific patterns, including different environmental drivers, diverse life histories, dispersal, and non-stationary dynamics. We find that traditional metrics for quantifying synchronous dynamics are often biased toward long-term drivers and may miss the importance of short-term drivers. Our findings indicate key mechanisms to consider when assessing synchronous versus compensatory dynamics and our approach provides a pathway for disentangling these dynamics in natural systems

Revisiting Generalized Chaplygin Gas as a Unified Dark Matter and Dark Energy Model

In this paper, we revisit generalized Chaplygin gas (GCG) model as a unified dark matter and dark energy model. The energy density of GCG model is given as $\rho_{GCG}/\rho_{GCG0}=[B_{s}+(1-B_{s})a^{-3(1+\alpha)}]^{1/(1+\alpha)}$, where $\alpha$ and $B_s$ are two model parameters which will be constrained by type Ia supernova as standard candles, baryon acoustic oscillation as standard rulers and the seventh year full WMAP data points. In this paper, we will not separate GCG into dark matter and dark energy parts any more as adopted in the literatures. By using Markov Chain Monte Carlo method, we find the result: $\alpha=0.00126_{- 0.00126- 0.00126}^{+ 0.000970+ 0.00268}$ and $B_s= 0.775_{- 0.0161- 0.0338}^{+ 0.0161+ 0.0307}$.Comment: 6 pages, 4 figure

Semaphorin 3F signaling actively retains neutrophils at sites of inflammation

Neutrophilic inflammation is central to disease pathogenesis, for example, in chronic obstructive pulmonary disease, yet the mechanisms that retain neutrophils within tissues remain poorly understood. With emerging evidence that axon guidance factors can regulate myeloid recruitment and that neutrophils can regulate expression of a class 3 semaphorin, SEMA3F, we investigated the role of SEMA3F in inflammatory cell retention within inflamed tissues. We observed that neutrophils upregulate SEMA3F in response to proinflammatory mediators and following neutrophil recruitment to the inflamed lung. In both zebrafish tail injury and murine acute lung injury models of neutrophilic inflammation, overexpression of SEMA3F delayed inflammation resolution with slower neutrophil migratory speeds and retention of neutrophils within the tissues. Conversely, constitutive loss of sema3f accelerated egress of neutrophils from the tail injury site in fish, whereas neutrophil-specific deletion of Sema3f in mice resulted in more rapid neutrophil transit through the airways, and significantly reduced time to resolution of the neutrophilic response. Study of filamentous-actin (F-actin) subsequently showed that SEMA3F-mediated retention is associated with F-actin disassembly. In conclusion, SEMA3F signaling actively regulates neutrophil retention within the injured tissues with consequences for neutrophil clearance and inflammation resolution

Recommendations to encourage participation of individuals from diverse backgrounds in psychiatric genetic studies

We present innovative research practices in psychiatric genetic studies to ensure representation of individuals from diverse ancestry, sex assigned at birth, gender identity, age, body shape and size, and socioeconomic backgrounds. Due to histories of inappropriate and harmful practices against marginalized groups in both psychiatry and genetics, people of certain identities may be hesitant to participate in research studies. Yet their participation is essential to ensure diverse representation, as it is incorrect to assume that the same genetic and environmental factors influence the risk for various psychiatric disorders across all demographic groups. We present approaches developed as part of the Eating Disorders Genetics Initiative (EDGI), a study that required tailored approaches to recruit diverse populations across many countries. Considerations include research priorities and design, recruitment and study branding, transparency, and community investment and ownership. Ensuring representation in participants is costly and funders need to provide adequate support to achieve diversity in recruitment in prime awards, not just as supplemental afterthoughts. The need for diverse samples in genetic studies is critical to minimize the risk of perpetuating health disparities in psychiatry and other health research. Although the EDGI strategies were designed specifically to attract and enroll individuals with eating disorders, our approach is broadly applicable across psychiatry and other fields