Efeito de fontes de óleo e níveis de suplementação de vitamina E na ração sobre as características físicas e morfológicas do sêmen in natura de suínos Effect of oil sources and vitamin E supplementation in the diet on physical and morphological characteristics of fresh boar semen

Avaliou-se o efeito da adição de diferentes fontes de óleo e níveis de suplementação de vitamina E na ração sobre as características do sêmen in natura de suínos reprodutores. Foram utilizados 24 reprodutores Dalboar 85, com idades entre 12 e 18 meses, distribuídos em delineamento inteiramente ao acaso, em arranjo fatorial 2 x 3, com duas fontes de óleo, soja e salmão, e três níveis de antioxidantes, 150, 300 e 450mg de vitamina E/kg. Volume, motilidade espermática total, teste hiposmótico, porcentagem de espermatozoides vivos e morfologia espermática não diferiram (P>0,05) entre os tratamentos. Óleo de salmão (P0,05). Os animais tratados com óleo de salmão apresentaram menor (PThe addition of oil sources and dietary vitamin E supplementation was evaluated on the characteristics of fresh boar sperm. Twenty-four mature Dalboar 85 boars of proven fertility and in routine semen production for artificial insemination were randomly distributed in a factorial arrangement 2 X 3, with two oil sources, soy and salmon, and three antioxidant levels: 150, 300, and 450mg/kg of vitamin E. Volume, total motile sperm, hyposmotic swelling test, percentage of live cell, and morphology did not differ (P0.05). Salmon oil treatment showed the lowest (P<0.05) concentration of total antioxidants in the semen. Vitamin E and concentration of total antioxidant showed a linear effect (P<0.05). The semen vigor and the concentration of total antioxidants were improved by oil sources, but the sperm concentration was dependent on the levels of vitamin E. Therefore, salmon oil can be used in the diet of male pigs

Crecimiento y supervivencia de postlarvas de piracanjuba "(Brycon orbignyanus)"

This work was conducted in the fish laboratory of the Environmental Station of Itutinga, CEMIG in January 2002. The objective was to evaluate the behavior of piracanjunba (Brycon orbignyanus) post-larvae at different stocking densities and feeding frequencies. The post-larvae were manteined into eighteen plastic rectangular- shaped boxes of 30 liters of volume. All the boxes posessed individual supply and flow of water with a constant renewal, and water temperature controlled (27°C), trough a heating system of the sort utilized in thermal pools. The experimental treatments consisted of three stocking densities (10, 20 and 30 post-larvae/liter) and two feeding frequencies (every 3 and 6 hours). The design was completely randomized with the treatments arranged into a 3 x 2 factorial scheme (stocking density x feeding frequency) with three replicates per treatment. The results showed that the increase of stocking density reduced linearly the fish"s survival. There were significant differences (p0,05). De acuerdo con los resultados, se concluye que la mejor supervivencia se obtiene con la densidad de 10 postlarvas/litro de piracanjuba, alimentadas de tres en tres horas

Comparative study of different techniques of evaluation of spermatic concentration in swines.

The precise and accurate determination of the spermatic concentration of the ejaculate is important in the production of insemination doses with a correct number of spermatozoa. The objective of this work was to compare the efficiency of some measurement techniques for spermatic concentration in the semen and to determine prediction equations, establishing a correlation among them. It were collected 93 ejaculations, its spermatic concentration was determined by direct (Neubauer chamber, NC) or indirect (spermdensimeter, SD and spectro-photometer, SP) methods. A randomized block design was utilized, with 93 repetitions and two analysis per experimental unit. Data were submitted to variance analysis, the SNK test was applied to means and the treatments were interrelated by means od adjustment of linear models among results (prediction equation). It was not difference in the determination of the spermatic concentration obtained with SD (361.7 x 106 sptz/ml) and SP (343.7 x 106 sptz/ml), but these values were higher than the NC (245.3 x 106 sptz/ml). The equations obtained were SD= -0.30 [ejaculate volume (in ml)] + 0.61 SP + 213.47 (R2= 0.72) and SD= -0.50 [ejaculate volume (in ml)] + 0.47 NC (R2= 0.7). The SD and SP measurement techniques of the spermatic concentration overestimate values with regard to CN. New research is need to determine the true efficiency of these techniques in farms.A determinação precisa e acurada da concentração espermática do ejaculado suíno é importante na produção de doses inseminantes com um número determinado de espermatozóides. O objetivo do trabalho foi comparar a eficiência de algumas técnicas de mensuração da concentração espermática no sêmen e determinar equações de predição, estabelecendo uma correlação entre elas. Foram coletados 93 ejaculados que tiveram as concentrações espermáticas determinadas de forma direta (câmara de Neubauer, CN) ou indireta (espermiodensímetro, ED e espectrofotômetro, EF). Foi utilizado um delineamento em blocos casualizados, com 93 repetições e duas análises por parcela experimental. Os valores foram submetidos à análise de variância, as médias submetidas ao teste SNK e os tratamentos interrelacionados pelo ajuste do modelo linear entre os dados (equações de predição). Não houve diferença na determinação da concentração espermática obtida com ED (361,7 x 106 sptz/ml) e EF (343,7 x 106 sptz/ml), porém estes valores foram maiores com relação a CN (245,3 x 106 sptz/ml). As equações obtidas foram: ED= -0,30 [volume (em ml)] + 0,61 EF + 213,47 (R2= 0,72) e ED= -0,50 [volume (ml)] + 0,47 CN (R2= 0,7). As técnicas de mensuração da concentração espermática pelo ED e EF superestimam os valores em relação à CN. Novos estudos devem ser conduzidos para determinar a verdadeira eficiência com que essas técnicas são empregadas a campo