Exogenous cadaverine induces oxidative burst and reduces cadaverine conjugate content in the common ice plant

The effect of free cadaverine (Cad) on its conjugates formation was analyzed in roots of the common ice plants (Mesembryanthemum crystallinum L.). It was found for the first time that Cad could induce oxidative burst in the roots of adult plants, as was evident from the sharp decrease in the content of Cad soluble or insoluble conjugates. This unusual effect was associated with the increased oxidative degradation of exogenous Cad (1 mM, 1.5 h) and intense H2O2 production in the root cells of adult plants. Root treatment of both juvenile and adult plants with H2O2 (1 mM, 1.5 h) reduced the content of soluble Cad conjugates and increased the content of their components, free Cad and phenols. We also found that one of the possible reasons of the negative effect of exogenous diamine on the formation of conjugated forms in adult roots was alkalization of the root apoplast at Cad addition to nutrient medium and the unusual O2 {radical dot}- synthase function as a pH-dependent guaiacol peroxidase in the presence of a high content of H2O2. This was confirmed by the data on the accumulation of O2 {radical dot}- and enhanced superoxide dismutase activity in adult roots under treatment with Cad. It is possible that the accumulation of O2 {radical dot}- together with H2O2 was also responsible for oxidative burst, which induced a decrease in the content of Cad conjugates in adult roots of the common ice plants. © 2008

Exogenous cadaverine induces oxidative burst and reduces cadaverine conjugate content in the common ice plant

The effect of free cadaverine (Cad) on its conjugates formation was analyzed in roots of the common ice plants (Mesembryanthemum crystallinum L.). It was found for the first time that Cad could induce oxidative burst in the roots of adult plants, as was evident from the sharp decrease in the content of Cad soluble or insoluble conjugates. This unusual effect was associated with the increased oxidative degradation of exogenous Cad (1 mM, 1.5 h) and intense H2O2 production in the root cells of adult plants. Root treatment of both juvenile and adult plants with H2O2 (1 mM, 1.5 h) reduced the content of soluble Cad conjugates and increased the content of their components, free Cad and phenols. We also found that one of the possible reasons of the negative effect of exogenous diamine on the formation of conjugated forms in adult roots was alkalization of the root apoplast at Cad addition to nutrient medium and the unusual O2 {radical dot}- synthase function as a pH-dependent guaiacol peroxidase in the presence of a high content of H2O2. This was confirmed by the data on the accumulation of O2 {radical dot}- and enhanced superoxide dismutase activity in adult roots under treatment with Cad. It is possible that the accumulation of O2 {radical dot}- together with H2O2 was also responsible for oxidative burst, which induced a decrease in the content of Cad conjugates in adult roots of the common ice plants. © 2008

Features of Oropharyngeal Microbiota in Children with Bronchial Asthma and Acute Respiratory Іnfection

The paper deals with the comparative analysis of composition of colonizing microflora in children with bronchial asthma (BA) and acute respiratory infection (ARI). The constant oropharyngeal biotopes in children with ARI and BA were Staphylococci and α-hemolytic Staphylococci. The arm groups differed by the content of secondary microorganisms: ARI were associated with Streptococcus spp. with β-hemolysis, E.coli and Candida spp., BA was associated with autochtonous microorganisms (Streptococcus spp. with β-hemolysis, Moraxella spp., Corynebacterium spp.). The variety of species composition of oropharyngeal biota was doubled in patients with ARI compared with the children with BA. Staphylococci and Streptococci prevailed at both ARI and BA

Calorimetry of an electronuclear target for uranium lead assembly at proton energy 1.5-GeV

El abordaje de la pertinencia de los instrumentos de desarrollo empresarial ofertados por las entidades que conforman la Comisión Regional de Competitividad e Innovación del Quindío, se hace con base en tres corrientes teóricas de la literatura académica: teorías y políticas de desarrollo local y regional, estrategias de competitividad y análisis de cadena de valor y clústeres. En el análisis de la información obtenida se pudo determinar que se están realizando ejercicios participativos de planificación estratégica con miras a fortalecer las capacidades competitivas de la estructura empresarial, pero la oferta de apoyos no tiene en cuenta la etapa del ciclo de vida de las empresas ni las especificidades del modelo de negocio donde compiten. También se evidencia la desarticulación interinstitucional y se refleja tímidamente en algunos sectores el enfoque orientado a incentivar interrelaciones entre empresas para estimular, fortalecer y desarrollar las capacidades competitivas. Finalmente es necesario crear un sistema territorial de información para estudiar las cadenas productivas y obtener datos estadísticos para diseñar las políticas de desarrollo productivo regional