In-sewer field-evaluation of an optical fibre-based condition monitoring system

A Fiber Bragg Grating (FBG) based monitoring system for continuous humidity and temperature measurement has been designed and evaluated experimentally in a sewer environment with high corrosion rates, humidity and the presence of gaseous hydrogen sulfide. The monitoring system has been designed specifically for field use, including packaging prepared for the harsh environment and the challenges of the operation. The system is battery powered and has hardware for controlling the interrogation equipment, power management, data logging and 4G connectivity. Results obtained show the long-term performance, over a 6-month period of non-stop monitoring of real-time data using the same probe. The data acquired was compared to the environmental data of temperature and precipitation for this period from the same location, which showed a good correlation between the expected and the measured data values. The data obtained point to the success of the optical fibre-based sensor system for monitoring in these harsh environments over long periods

Predictive Analytics Toolkit for H2S Estimation and Sewer Corrosion

This paper presents a predictive analytics toolkit, which is based on the emerging spatiotemporal data analysis techniques, for the estimation of hydrogen sulphide (H2S) gas distribution and prediction of sewer concrete corrosion level. The toolkit is an easy-to-use desktop application with a user-friendly interface for querying and producing output results on GIS. The inputs to the toolkit are the sewer network geometry, monitored factors, and hydraulic information; the outputs of the toolkit are spatiotemporal estimates of H2S gas concentration and concrete corrosion levels on the entire sewer network with uncertainties of the predictions. The toolkit is also able to integrate experts’ domain knowledge or existing physical model’s results as prior knowledge into the analytics model. The final outcomes of the toolkit can be used to prioritise high risk areas, recommend chemical dosing locations, and suggest deployment of sensors. A simulation of H2S and corrosion level prediction on a subsystem of the sewer network in the greater Sydney area is reported to demonstrate the capability of the toolki

Extended study of fiber optic-based humidity sensing system performance for sewer network condition monitoring

This paper reports on an extended (20-month) period of monitoring of humidity in situ at two locations in the sewer network operated by Sydney Water using a fibre optic network into which a series of Bragg Grating-based sensors had been installed. The locations (Eustace Street in Manly, Sydney and Old Toongabbie at Oakes Reserve, western Sydney, Australia) both had different operating environments and thus conditions for evaluating the sensor system. It was designed to provide a solution to enable long term, low cost and more reliable monitoring in the harsh conditions of the sewer environments in terms of high relative humidity > 95% and a broad range of hydrogen sulfide levels. The results of the study show that even after ~20 months of use, the same sensor is reliably recording humidity and temperature in the sewer environment – overcoming the problems seen with conventional electrical sensors, which typically fail within a couple of weeks of use in this continuous high acid/high humidity environment. The data, recorded constantly from the sensor system, were stable throughout the full monitoring period and further, a comparison with the changing weather conditions was made over the different seasons during the study. The sensor system developed was battery operated and had 4G connectivity for data transfer and debugging. These features have enabled the system to be installed in situations where power is not available and operate successfully with minimal human operation, thus allowing for additional systems to be integrated to the measurement system in the future

Heterologous Replacement of the Supposed Host Determining Region of Avihepadnaviruses: High In Vivo Infectivity Despite Low Infectivity for Hepatocytes

Hepadnaviruses, including hepatitis B virus (HBV), a highly relevant human pathogen, are small enveloped DNA viruses that replicate via reverse transcription. All hepadnaviruses display a narrow tissue and host tropism. For HBV, this restricts efficient experimental in vivo infection to chimpanzees. While the cellular factors mediating infection are largely unknown, the large viral envelope protein (L) plays a pivotal role for infectivity. Furthermore, certain segments of the PreS domain of L from duck HBV (DHBV) enhanced infectivity for cultured duck hepatocytes of pseudotyped heron HBV (HHBV), a virus unable to infect ducks in vivo. This implied a crucial role for the PreS sequence from amino acid 22 to 90 in the duck tropism of DHBV. Reasoning that reciprocal replacements would reduce infectivity for ducks, we generated spreading-competent chimeric DHBVs with L proteins in which segments 22–90 (Du-He4) or its subsegments 22–37 and 37–90 (Du-He2, Du-He3) are derived from HHBV. Infectivity for duck hepatocytes of Du-He4 and Du-He3, though not Du-He2, was indeed clearly reduced compared to wild-type DHBV. Surprisingly, however, in ducks even Du-He4 caused high-titered, persistent, horizontally and vertically transmissable infections, with kinetics of viral spread similar to those of DHBV when inoculated at doses of 108 viral genome equivalents (vge) per animal. Low-dose infections down to 300 vge per duck did not reveal a significant reduction in specific infectivity of the chimera. Hence, sequence alterations in PreS that limited infectivity in vitro did not do so in vivo. These data reveal a much more complex correlation between PreS sequence and host specificity than might have been anticipated; more generally, they question the value of cultured hepatocytes for reliably predicting in vivo infectivity of avian and, by inference, mammalian hepadnaviruses, with potential implications for the risk assessment of vaccine and drug resistant HBV variants