3,541 research outputs found
Laser driven self-assembly of shape-controlled potassium nanoparticles in porous glass
We observe growth of shape-controlled potassium nanoparticles inside a random
network of glass nanopores, exposed to low-power laser radiation. Visible laser
light plays a dual role: it increases the desorption probability of potassium
atoms from the inner glass walls and induces the self-assembly of metastable
metallic nanoparticles along the nanopores. By probing the sample transparency
and the atomic light-induced desorption flux into the vapour phase, the
dynamics of both cluster formation/evaporation and atomic photo-desorption
processes are characterized. Results indicate that laser light not only
increases the number of nanoparticles embedded in the glass matrix but also
influences their structural properties. By properly choosing the laser
frequency and the illumination time, we demonstrate that it is possible to
tailor the nanoparticles'shape distribution. Furthermore, a deep connection
between the macroscopic behaviour of atomic desorption and light-assisted
cluster formation is observed. Our results suggest new perspectives for the
study of atom/surface interaction as well as an effective tool for the
light-controlled reversible growth of nanostructures.Comment: 14 pages,6 figures, http://iopscience.iop.org/1612-202X/11/8/085902
The n-acetyl phenylalanine glucosamine derivative attenuates the inflammatory/catabolic environment in a chondrocyte-synoviocyte co-culture system
Osteoarthritis (OA), the most prevalent degenerative joint disease, still lacks a true disease-modifying therapy. The involvement of the NF-κB pathway and its upstream activating kinases in OA pathogenesis has been recognized for many years. The ability of the N-acetyl phenylalanine glucosamine derivative (NAPA) to increase anabolism and reduce catabolism via inhibition of IKKα kinase has been previously observed in vitro and in vivo. The present study aims to confirm the chondroprotective effects of NAPA in an in vitro model of joint OA established with primary cells, respecting both the crosstalk between chondrocytes and synoviocytes and their phenotypes. This model satisfactorily reproduces some features of the previously investigated DMM model, such as the prominent induction of ADAMTS-5 upon inflammatory stimulation. Both gene and protein expression analysis indicated the ability of NAPA to counteract key cartilage catabolic enzymes (ADAMTS-5) and effectors (MCP-1). Molecular analysis showed the ability of NAPA to reduce IKKα nuclear translocation and H3Ser10 phosphorylation, thus inhibiting IKKα transactivation of NF-κB signalling, a pivotal step in the NF-κB-dependent gene expression of some of its targets. In conclusion, our data confirm that NAPA could truly act as a disease-modifying drug in OA
Ab initio analysis of the x-ray absorption spectrum of the myoglobin-carbon monoxide complex: Structure and vibrations
We present a comparison between Fe K-edge x-ray absorption spectra of
carbonmonoxy-myoglobin and its simulation based on density-functional theory
determination of the structure and vibrations and spectral simulation with
multiple-scattering theory. An excellent comparison is obtained for the main
part of the molecular structure without any structural fitting parameters. The
geometry of the CO ligand is reliably determined using a synergic approach to
data analysis. The methodology underlying this approach is expected to be
especially useful in similar situations in which high-resolution data for
structure and vibrations are available.Comment: 13 pages, 3 figure
Genetic Variability of Lucerne Landraces from Central Italy Detected by RAPD Markers
With the aim to characterize six lucerne landraces (Medicago sativa L.), representing a sample of a collection from central Italy, sixty individuals per landrace were evaluated by screening for RAPD markers with three lucerne-specific primers. Twenty-one amplification products were scored as present or absent across all plants. The dendrogram from mean genetic similarity estimates displayed Casalina alone and the other landraces clustered into one distinct group, showing a single branch point with more than 73% of genetic similarity. The discriminant analysis grouped the landraces in a similar manner. The first function maximally separated the group Grosseto, Gubbio and C. Pieve from Latina and L’Aquila while the second function maximally separated Casalina from the rest of landraces. Overall 56% of individual plants were correctly reclassified into their own groups. Owing to their rather narrow geographic provenance, more primers are needed to increase precision in the estimate of the genetic variability
PCV83 ADHERENCE WITH ANTIHYPERTENSIVE DRUG TREATMENT: EVIDENCE FROM PRIMARY CARE PRACTICE IN ITALY
Fantastic animals as an experimental model to teach animal adaptation
Background: Science curricula and teachers should emphasize evolution in a mannercommensurate with its importance as a unifying concept in science. The concept of adaptationrepresents a first step to understand the results of natural selection. We settled an experimentalproject of alternative didactic to improve knowledge of organism adaptation. Students wereinvolved and stimulated in learning processes by creative activities. To set adaptation in a historicframe, fossil records as evidence of past life and evolution were considered.Results: The experimental project is schematized in nine phases: review of previous knowledge;lesson on fossils; lesson on fantastic animals; planning an imaginary world; creation of an imaginaryanimal; revision of the imaginary animals; adaptations of real animals; adaptations of fossil animals;and public exposition. A rubric to evaluate the student's performances is reported. The projectinvolved professors and students of the University of Modena and Reggio Emilia and of the "G.Marconi" Secondary School of First Degree (Modena, Italy).Conclusion: The educational objectives of the project are in line with the National Indications ofthe Italian Ministry of Public Instruction: knowledge of the characteristics of living beings, themeanings of the term "adaptation", the meaning of fossils, the definition of ecosystem, and theparticularity of the different biomes. At the end of the project, students will be able to graspparticular adaptations of real organisms and to deduce information about the environment in whichthe organism evolved. This project allows students to review previous knowledge and to form theirpersonalities
DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function
Parkinson's disease is a neurodegenerative disorder characterized by the death of dopaminergic neurons and by accumulation of alpha-synuclein (aS) aggregates in the surviving neurons. The dopamine catabolite 3,4-dihydroxyphenylacetaldehyde (DOPAL) is a highly reactive and toxic molecule that leads to aS oligomerization by covalent modifications to lysine residues. Here we show that DOPAL-induced aS oligomer formation in neurons is associated with damage of synaptic vesicles, and with alterations in the synaptic vesicles pools. To investigate the molecular mechanism that leads to synaptic impairment, we first aimed to characterize the biochemical and biophysical properties of the aS-DOPAL oligomers; heterogeneous ensembles of macromolecules able to permeabilise cholesterol-containing lipid membranes. aS-DOPAL oligomers can induce dopamine leak in an in vitro model of synaptic vesicles and in cellular models. The dopamine released, after conversion to DOPAL in the cytoplasm, could trigger a noxious cycle that further fuels the formation of aS-DOPAL oligomers, inducing neurodegeneration
Microwave-assisted preparation of multi principal element alloys by powder metallurgy approach
According to literature, the synthetic route to produce High entropy alloys (HEAs) should guarantee short alloying time, efficient cooling and capability to operate in controlled atmosphere. Such conditions can be achieved using high frequency electromagnetic fields, like microwave heating. In this work FeCoNiCrAl and FeCoNiCuAl, both equiatomic and reinforced by the 10% wt. of SiC were prepared by microwave assisted techniques. Results show that direct microwave heating of the powder precursors occurs, until the ignition conditions are reached. The temperature and duration of the microwave-assisted process result much lower than other conventional powder metallurgy routes, but at the cost of a higher residual porosity. Sample characterization confirmed that the powder metallurgy approach is suitable to retain the shape of the load imparted during forming by uniaxial pressing. The homogeneity of the samples resulted in being good in all cases, without the dendritic segregation typically occurring by liquid phase processing. © 2017 European Powder Metallurgy Association (EPMA
Clinical efficacy of medical hydrology: an umbrella review
The aim of this research was to summarize available scientific evidence on the efficacy of medical hydrology for the management of any health condition. The search was conducted on 26th March 2021, in the following databases: Medline (via PubMed), EMBASE, Web of Science, Cochrane Library, and Google Scholar. All relevant literature reviews investigating the clinical efficacy of interventions characterized by the use of natural mineral waters and muds were included. The quality of studies was assessed with the “AMSTAR 2” tool. After article screening, 49 reviews were included in this work. Overall, retrieved scientific evidence suggests that spa therapy is beneficial for patients affected by some specific musculoskeletal conditions, with improvements potentially lasting up to 9 months. Moreover, balneotherapy can be an integrative support for the management of chronic venous insufficiency and some inflammatory skin diseases like psoriasis. The role of spa therapy in rehabilitation appears relevant as well. More limited, although interesting evidence exists for inhalation and hydropinic therapies. Globally, retrieved evidence suggests that, besides individual wellbeing, medical hydrology can be useful for public health. In particular, higher-quality studies seem to support the integrative use of spa-related interventions for conditions like osteoarthritis, fibromyalgia, low back pain of rheumatic origin, and chronic venous insufficiency. However, the body of evidence has some limitations and further clinical trials should be designed for each relevant application to consolidate and expand acquired knowledge
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