53 research outputs found
Subnanosecond spectral diffusion measurement using photon correlation
Spectral diffusion is a result of random spectral jumps of a narrow line as a
result of a fluctuating environment. It is an important issue in spectroscopy,
because the observed spectral broadening prevents access to the intrinsic line
properties. However, its characteristic parameters provide local information on
the environment of a light emitter embedded in a solid matrix, or moving within
a fluid, leading to numerous applications in physics and biology. We present a
new experimental technique for measuring spectral diffusion based on photon
correlations within a spectral line. Autocorrelation on half of the line and
cross-correlation between the two halves give a quantitative value of the
spectral diffusion time, with a resolution only limited by the correlation
set-up. We have measured spectral diffusion of the photoluminescence of a
single light emitter with a time resolution of 90 ps, exceeding by four orders
of magnitude the best resolution reported to date
Recent Advances in Graph Partitioning
We survey recent trends in practical algorithms for balanced graph
partitioning together with applications and future research directions
Diffraction techniques and vibrational spectroscopy opportunities to characterise bones
From a histological point of view, bones that allow body mobility and protection of internal organs consist not only of different organic and inorganic tissues but include vascular and nervous elements as well. Moreover, due to its ability to host different ions and cations, its mineral part represents an important reservoir, playing a key role in the metabolic activity of the organism. From a structural point of view, bones can be considered as a composite material displaying a hierarchical structure at different scales. At the nanometre scale, an organic part, i.e. collagen fibrils and an inorganic part, i.e. calcium phosphate nanocrystals are intimately mixed to assure particular mechanical properties
Desk research of 107 case studies on state-of-the-art of cultural tourism interventions.
Cultural tourism; cultural heritage; case study; tourism interventions
SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation
Despite growing demand for truly naïve imaging, label-free observation of cilium-related structure remains challenging, and validation of the pertinent molecules is correspondingly difficult. In this study, in retinas and cultured cells, we distinctively visualized Rootletin filaments in rootlets in the second harmonic generation (SHG) channel, integrated in custom coherent nonlinear optical microscopy (CNOM) with a simple, compact, and ultra-broadband supercontinuum light source. This SHG signal was primarily detected on rootlets of connecting cilia in the retinal photoreceptor and was validated by colocalization with anti-Rootletin staining. Transfection of cells with Rootletin fragments revealed that the SHG signal can be ascribed to filaments assembled from the R234 domain, but not to cross-striations assembled from the R123 domain. Consistent with this, Rootletin-depleted cells lacked SHG signal expected as centrosome linker. As a proof of concept, we confirmed that similar fibrous SHG was observed even in unicellular ciliates. These findings have potential for broad applications in clinical diagnosis and biophysical experiments with various organisms
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