Free and conjugated androgen and progestin levels in the serum of stellate sturgeon (Acipenser stellatus Pallas) males treated with female coelomic fluid

Based on the supposition that female coelomic fluid contents have a certain chemical influence on sturgeons, blood serum concentrations of free and conjugated testosterone (T), 11-ketotestosterone (11KT), 17,20b,21-trihydroxy-4-pregnen- 3-one (20bS), 17,20b-dihydroxy-4-pregnen-3-one (DHP), 11-desoxycortisol (S) and free progesterone (P4) have been measured by radioimmunoassay (RIA) and enzyme-linked immuno-sorbent assay (ELISA) in stellate sturgeon (Acipenser stellatus Pallas) males treated with female coelomic fluid (CF); CF steroid levels have also been analyzed. After treatment a significant elevation of free 20bS and glucuronidated DHP and S levels and the decrease of free T and KT levels have been observed. The obtained data support the idea that the components of CF may play some part in pheromonal effects for sturgeon reproduction

Levels of free and conjugated androgens and progestins in coelomic fluid and serum of stellate sturgeon (Acipenser stellatus Pallas) females

Stellate sturgeon (Acipenser stellatus Pallas) females from the Volga River were analysed for sex steroids after hormonal stimulation. The results show a significant increase of sulfated sex steroid levels in serum and coelomic fluid (CF) at final maturation (FM). It is hypothesized that the steroid metabolites could be part of a chemical communication system related to reproduction

Free androgens and progestins and their conjugated forms in serum and urine of stellate sturgeon (Acipenser stellatus Pallas) males

Stellate sturgeon (Acipenser stellatus Pallas) males from Volga River treated with LH-RH-A showed an increase of free, sulfated and glucuronided sex steroid levels in serum and urine at spermiation. Conjugated forms of sex steroids could act as pheromones in sturgeon

Effects of alpha fetoprotein on escape of Bel 7402 cells from attack of lymphocytes

BACKGROUND: Involvement of AFP against apoptosis of tumor cell has been implicated in its evasion of immune surveillance. However, the molecular events of immune escape mechanisms are still unknown. The major observations reported here relate to a possible mechanism by which heptoloma Bel 7402 cells escape immune surveillance in vitro. METHODS: Western blotting and a well-characterized cofocal scanning image were performed to analyze the expression of Fas/FasL and caspase-3 in co-cultured Bel 7402 and Jurkat cells. RESULTS: After co-culture with Jurkat cells, up-regulated Fas and reduced FasL expression could be observed. Treatment with AFP could remarkably inhibit the elevated Fas and, whereas, induce the FasL expression in co-cultured Bel 7402 cells. Cells co-culture could induce the expression of caspase-3 in both cells line. The elevated caspase-3 in Bel 7402 cells was abolished following the treatment of AFP. The expression of caspase-3 was elevated in co-cultured Jurkat cells treated with AFP. No detectable change on the expression of survivin was examined in both cells line. Monoclonal antibody against AFP treatment alone did not obviously influence the growth of cells, as well as the expression of Fas/FasL and caspase-3. However, the effect of AFP could be blocked by antibody. CONCLUSIONS: our results provide evidence that AFP could promote the escape of liver cancer cells from immune surveillance through blocking the caspase signal pathway of tumor cells and triggering the Fas/FasL interaction between tumor cells and lymphocytes

Клиническая оценка эффективности кашля у больных неспецифическими заболеваниями легких на основе туссографии и времени экспекторации

The article deals with the investigation of cough and mico-cilliary clearence in patients with nonspecific iung diseases (lobular pneumonia, lung abscess, intrinsic asthma). The aim of this study is to define quantitative criteria of cough efficacy which in terms give the integral characteristic of tracheobronchial clearence components. It is achieved by monitoring the time of inuline expectoration and registering of cough jolts with the help of tussograph IKT-1. In case of prolongation of inuline expectoration time using drug suppression of cough, the latter is regarded effective.

The Apoptosome: Emerging Insights and New Potential Targets for Drug Design

Apoptosis plays a crucial role in tissue homeostasis, development and many diseases. The relevance of Apaf1, the molecular core of apoptosome, has been underlined in mitochondria-dependent apoptosis, which according to a growing body of evidence, is involved in various pathologies where the equilibrium of life-and-death is dysregulated, such as heart attack, stroke, liver failure, cancer and autoimmune diseases. Consequently, great interest has emerged in devising therapeutic strategies for regulating the key molecules involved in the life-and-death decision. Here we review recent progress in apoptosis-based pharmacological therapies and, in particular, we point out a possible role of the apoptosome as an emerging and promising pharmacological target

General types of idiomatic verbal units in English and Swahili

The article deals with the comparative analysis of the idiomatic verbal units-word combination of the two typologically different languages - English and Swahili: As a result a number of general syntactic types of their idiomatic verbal units are determined

The formation and specific features of Swahili socio-political newspaper vocabulary

The article deals with a number of questions concerning Swahili socio-political newspaper vocabulary - its forming and development, the appearance of neologisms and means of new word formation, figurative expressions used in the texts

The Role of Borrowings in the Development of Swahili Vocabulary (on the Material of Arabic Loan Words)

The article deals with the problems of Swahili vocabulary development by means of borrowings from the Arab language. A lot of borrowed words and word combinations belonging to different semantic groups are being analyzed here