Engineering biological gradients

Biological gradients profoundly influence many cellular activities, such as adhesion, migration, and differentiation, which are the key to biological processes, such as inflammation, remodeling, and tissue regeneration. Thus, engineered structures containing bioinspired gradients can not only support a better understanding of these phenomena, but also guide and improve the current limits of regenerative medicine. In this review, we outline the challenges behind the engineering of devices containing chemical-physical and biomolecular gradients, classifying them according to gradient-making methods and the finalities of the systems. Different manufacturing processes can generate gradients in either in-vitro systems or scaffolds, which are suitable tools for the study of cellular behavior and for regenerative medicine; within these, rapid prototyping techniques may have a huge impact on the controlled production of gradients. The parallel need to develop characterization techniques is addressed, underlining advantages and weaknesses in the analysis of both chemical and physical gradients

Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics

Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue. Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time. Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen). Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered. Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments. By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition. In addition, the results indicate that not only cells survive and proliferate following 3D-bioprinting, but they can also interact and reorganize hydrogel microstructure. Taken together, the results suggest that pectin-based hydrogels could be successfully applied for neural cell culture

Asymptotics of LQG fusion coefficients

The fusion coefficients from SO(3) to SO(4) play a key role in the definition of spin foam models for the dynamics in Loop Quantum Gravity. In this paper we give a simple analytic formula of the EPRL fusion coefficients. We study the large spin asymptotics and show that they map SO(3) semiclassical intertwiners into $SU(2)_L\times SU(2)_R$ semiclassical intertwiners. This non-trivial property opens the possibility for an analysis of the semiclassical behavior of the model.Comment: 14 pages, minor change

Gel for the regeneration of mucosa

The object of the present invention is a composition in the form of a gel which comprises aloe vera juice and pectin in a concentration of between 0.1 and 3%, preferably about 0.3%. Optionally, said composition comprises further active ingredients. A further object of the present invention is said composition for use in the local treatment of inflammatory states of the mucosa

Gel per la rigenerazione di mucose

Forma oggetto della presente invenzione una composizione in forma di gel che comprende succo di aloe vera e pectina in concentrazione compresa tra 0,1 e 3%, preferibilmente circa 0,3%. Opzionalmente, detta composizione comprende ulteriori attivi. Forma ulteriore oggetto della presente invenzione detta composizione per l’uso nel trattamento locale di stati infiammatori delle mucose

Advanced organ-on-a-chip devices to investigate liver multi-organ communication: Focus on gut, microbiota and brain

The liver is a key organ that can communicate with many other districts of the human body. In the last few decades, much interest has focused on the interaction between the liver and the gut microbiota, with their reciprocal influence on biosynthesis pathways and the integrity the intestinal epithelial barrier. Dysbiosis or liver disorders lead to0 epithelial barrier dysfunction, altering membrane permeability to toxins. Clinical and experimental evidence shows that the permeability hence the delivery of neurotoxins such as LPS, ammonia and salsolinol contribute to neurological disorders. These findings suggested multi-organ communication between the gut microbiota, the liver and the brain. With a view to in vitro modeling this liver-based multi-organ communication, we describe the latest advanced liver-on-a-chip devices and discuss the need for new organ-on-a-chip platforms for in vitro modeling the in vivo multi-organ connection pathways in physiological and pathological situations