Radiance and Doppler shift distributions across the network of the quiet Sun

The radiance and Doppler-shift distributions across the solar network provide observational constraints of two-dimensional modeling of transition-region emission and flows in coronal funnels. Two different methods, dispersion plots and average-profile studies, were applied to investigate these distributions. In the dispersion plots, we divided the entire scanned region into a bright and a dark part according to an image of Fe xii; we plotted intensities and Doppler shifts in each bin as determined according to a filtered intensity of Si ii. We also studied the difference in height variations of the magnetic field as extrapolated from the MDI magnetogram, in and outside network. For the average-profile study, we selected 74 individual cases and derived the average profiles of intensities and Doppler shifts across the network. The dispersion plots reveal that the intensities of Si ii and C iv increase from network boundary to network center in both parts. However, the intensity of Ne viii shows different trends, namely increasing in the bright part and decreasing in the dark part. In both parts, the Doppler shift of C iv increases steadily from internetwork to network center. The average-profile study reveals that the intensities of the three lines all decline from the network center to internetwork region. The binned intensities of Si ii and Ne viii have a good correlation. We also find that the large blue shift of Ne viii does not coincide with large red shift of C iv. Our results suggest that the network structure is still prominent at the layer where Ne viii is formed in the quiet Sun, and that the magnetic structures expand more strongly in the dark part than in the bright part of this quiet Sun region.Comment: 10 pages,9 figure

Upflows in the upper transition region of the quiet Sun

We investigate the physical meaning of the prominent blue shifts of Ne VIII, which is observed to be associated with quiet-Sun network junctions (boundary intersections), through data analyses combining force-free-field extrapolations with EUV spectroscopic observations. For a middle-latitude region, we reconstruct the magnetic funnel structure in a sub-region showing faint emission in EIT-Fe 195. This funnel appears to consist of several smaller funnels that originate from network lanes, expand with height and finally merge into a single wide open-field region. However, the large blue shifts of Ne VIII are generally not associated with open fields, but seem to be associated with the legs of closed magnetic loops. Moreover, in most cases significant upflows are found in both of the funnel-shaped loop legs. These quasi-steady upflows are regarded as signatures of mass supply to the coronal loops rather than the solar wind. Our observational result also reveals that in many cases the upflows in the upper transition region (TR) and the downflows in the middle TR are not fully cospatial. Based on these new observational results, we suggest different TR structures in coronal holes and in the quiet Sun.Comment: 4 pages, 4 figures, will appear in the Proceedings of the Solar wind 12 conferenc

The role of Joule heating in the formation of nanogaps by electromigration

We investigate the formation of nanogaps in gold wires due to electromigration. We show that the breaking process will not start until a local temperature of typically 400 K is reached by Joule heating. This value is rather independent of the temperature of the sample environment (4.2-295 K). Furthermore, we demonstrate that the breaking dynamics can be controlled by minimizing the total series resistance of the system. In this way, the local temperature rise just before break down is limited and melting effects are prevented. Hence, electrodes with gaps < 2 nm are easily made, without the need of active feedback. For optimized samples, we observe quantized conductance steps prior the gap formation.Comment: including 7 figure

Differential Distribution of Ca2+-Activated K+ Channel Splice Variants among Hair Cells along the Tonotopic Axis of the Chick Cochlea

AbstractSummary We have cloned from the receptor epithelium of the chick cochlea a family of alternatively spliced cDNAs derived from cslo, which encodes a Ca2+-activated K+ channel like those shown to help determine the resonant frequency of electrically tuned hair cells. Our results from PCRs using template RNAs from both tonotopically subdivided receptor epithelia and single hair cells demonstrate differential exon usage along the frequency axis of the epithelium at multiple splice sites in cslo. We also show that single hair cells express more than one splice variant at a given splice site. Since channel isoforms encoded by differentially spliced slo transcripts in other species are functionally heterogeneous, these data suggest that differential processing of slo transcripts may account, at least in part, for the systematic variation in hair-cell membrane properties along the frequency axis of electrically tuned auditory receptor epithelia

Non-Hausdorff Symmetries of C*-algebras

Symmetry groups or groupoids of C*-algebras associated to non-Hausdorff spaces are often non-Hausdorff as well. We describe such symmetries using crossed modules of groupoids. We define actions of crossed modules on C*-algebras and crossed products for such actions, and justify these definitions with some basic general results and examples.Comment: very minor changes. To appear in Math. An

Trypanosoma brucei PRMT1 Is a Nucleic Acid Binding Protein with a Role in Energy Metabolism and the Starvation Stress Response.

In Trypanosoma brucei and related kinetoplastid parasites, transcription of protein coding genes is largely unregulated. Rather, mRNA binding proteins, which impact processes such as transcript stability and translation efficiency, are the predominant regulators of gene expression. Arginine methylation is a posttranslational modification that preferentially targets RNA binding proteins and is, therefore, likely to have a substantial impact on T. brucei biology. The data presented here demonstrate that cells depleted of T. brucei PRMT1 (TbPRMT1), a major type I protein arginine methyltransferase, exhibit decreased virulence in an animal model. To understand the basis of this phenotype, quantitative global proteomics was employed to measure protein steady-state levels in cells lacking TbPRMT1. The approach revealed striking changes in proteins involved in energy metabolism. Most prominent were a decrease in glycolytic enzyme abundance and an increase in proline degradation pathway components, changes that resemble the metabolic remodeling that occurs during T. brucei life cycle progression. The work describes several RNA binding proteins whose association with mRNA was altered in TbPRMT1-depleted cells, and a large number of TbPRMT1-interacting proteins, thereby highlighting potential TbPRMT1 substrates. Many proteins involved in the T. brucei starvation stress response were found to interact with TbPRMT1, prompting analysis of the response of TbPRMT1-depleted cells to nutrient deprivation. Indeed, depletion of TbPRMT1 strongly hinders the ability of T. brucei to form cytoplasmic mRNA granules under starvation conditions. Finally, this work shows that TbPRMT1 itself binds nucleic acids in vitro and in vivo, a feature completely novel to protein arginine methyltransferases.IMPORTANCETrypanosoma brucei infection causes human African trypanosomiasis, also known as sleeping sickness, a disease with a nearly 100% fatality rate when untreated. Current drugs are expensive, toxic, and highly impractical to administer, prompting the community to explore various unique aspects of T. brucei biology in search of better treatments. In this study, we identified the protein arginine methyltransferase (PRMT), TbPRMT1, as a factor that modulates numerous aspects of T. brucei biology. These include glycolysis and life cycle progression signaling, both of which are being intensely researched toward identification of potential drug targets. Our data will aid research in those fields. Furthermore, we demonstrate for the first time a direct association of a PRMT with nucleic acids, a finding we believe could translate to other organisms, including humans, thereby impacting research in fields as distant as human cancer biology and immune response modulation. Copyright © 2018 Kafková et al

Effect of periodic parametric excitation on an ensemble of force-coupled self-oscillators

We report the synchronization behavior in a one-dimensional chain of identical limit cycle oscillators coupled to a mass-spring load via a force relation. We consider the effect of periodic parametric modulation on the final synchronization states of the system. Two types of external parametric excitations are investigated numerically: periodic modulation of the stiffness of the inertial oscillator and periodic excitation of the frequency of the self-oscillatory element. We show that the synchronization scenarios are ruled not only by the choice of parameters of the excitation force but depend on the initial collective state in the ensemble. We give detailed analysis of entrainment behavior for initially homogeneous and inhomogeneous states. Among other results, we describe a regime of partial synchronization. This regime is characterized by the frequency of collective oscillation being entrained to the stimulation frequency but different from the average individual oscillators frequency.Comment: Comments and suggestions are welcom