21 research outputs found
Metal-Free g-C3N4/Nanodiamond Heterostructures for Enhanced Photocatalytic Pollutant Removal and Bacteria Photoinactivation
Heterogeneous photocatalysis has emerged as a promising alternative for both micropollutant removal and bacterial inactivation under solar irradiation. Among a variety of photocatalysts explored in the literature, graphite carbon nitride (g-C3N4) is a metal-free semiconductor with acceptable chemical stability, low toxicity, and excellent cost-effectiveness. To minimize its high charge recombination rate and increase the photocatalyst adsorption capacity whilst keeping the metal-free photocatalyst system idea, we proposed the heterojunction formation of g-C3N4 with diamond nanocrystals (DNCs), also known as nanodiamonds. Samples containing different amounts of DNCs were assessed as photocatalysts for pollutant removal from water and as light-activated antibacterial agents against Staphylococcus sureus. The sample containing 28.3 wt.% of DNCs presented the best photocatalytic efficiency against methylene blue, removing 71% of the initial dye concentration after 120 min, with a pseudo-first-order kinetic and a constant rate of 0.0104 min−1, which is nearly twice the value of pure g-C3N4 (0.0059 min−1). The best metal-free photocatalyst was able to promote an enhanced reduction in bacterial growth under illumination, demonstrating its capability of photocatalytic inactivation of Staphylococcus aureus. The enhanced photocatalytic activity was discussed and attributed to (i) the increased adsorption capacity promoted by the presence of DNCs; (ii) the reduced charge recombination rate due to a type-II heterojunction formation; (iii) the enhanced light absorption effectiveness; and (iv) the better charge transfer resistance. These results show that g-C3N4/DNC are low-cost and metal-free photoactive catalysts for wastewater treatment and inactivation of bacteria
Lipoxygenase Inhibitors Nordihydroguaiaretic Acid and Fungus Lecanicillum lecanii Extract Induce Death of Lymphoid Leukemia Cells
Blood Pressure Changes After Exposures Increasing Angiotensin-Converting Enzyme Activity and After Its Normalization with Dihydroquercetin in Male Wistar Rats
In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood Testes in vitro de plantas Amazônicas para atividade hemolítica e inibição da agregação plaquetária em sangue humano
In the present study, different aerial parts from twelve Amazonian plant species found in the National Institute for Amazon Research's (INPA's) Adolpho Ducke Forest Reserve (in Manaus, Amazonas, Brazil) were collected. Separate portions of dried, ground plant materials were extracted with water (by infusion), methanol and chloroform (by continuous liquid-solid extraction) and solvents were removed first by rotary evaporation, and finally by freeze-drying which yielded a total of seventy-one freeze-dried extracts for evaluation. These extracts were evaluated initially at concentrations of 500 and 100 µg/mL for in vitro hemolytic activity and in vitro inhibition of platelet aggregation in human blood, respectively. Sixteen extracts (23 % of all extracts tested, 42 % of all plant species), representing the following plants: Chaunochiton kappleri (Olacaceae), Diclinanona calycina (Annonaceae), Paypayrola grandiflora (Violaceae), Pleurisanthes parviflora (Icacinaceae), Sarcaulus brasiliensis (Sapotaceae), exhibited significant inhibitory activity towards human platelet aggregation. A group of extracts with antiplatelet aggregation activity having no in vitro hemolytic activity has therefore been identified. Three extracts (4 %), all derived from Elaeoluma nuda (Sapotaceae), exhibited hemolytic activity. None of the plant species in this study has known use in traditional medicine. So, these data serve as a baseline or minimum of antiplatelet and hemolytic activities (and potential usefulness) of non-medicinal plants from the Amazon forest. Finally, in general, these are the first data on hemolytic and inhibitory activity on platelet aggregation for the genera which these plant species represent.<br>No presente estudo, partes aéreas obtidas de doze (12) espécies vegetais da Amazônia encontradas na Reserva Florestal Adolpho Ducke (localizada na cidade de Manaus, Estado do Amazonas, Brasil) do Instituto Nacional de Pesquisas da Amazônia foram coletadas, secadas e moídas. Porções dos materiais vegetais em pó foram extraídas com água (por infusão), metanol e clorofórmio (por extração líquido-sólido contínua) e os solventes foram removidos por evaporação rotatória e finalmente liofilização, forneceram um total de setenta e one (71) extratos liofilizados. Esses extratos foram avaliados inicialmente para atividade hemolítica in vitro e atividade inibitória da agregação plaquetária em sangue humano in vitro em concentrações de 500 e 100 µg/mL, respectivamente. Dezesseis (16) extratos (23 % dos extratos testados, 42 % das espécies vegetais) representando as seguintes plantas, apresentaram inibição significativa frente a agregação de plaquetas humanas in vitro: Chaunochiton kappleri (Olacaceae), Diclinanona calycina (Annonaceae), Paypayrola grandiflora (Violaceae), Pleurisanthes parviflora (Icacinaceae), Sarcaulus brasiliensis (Sapotaceae). Como principal resultado, um grupo de extratos apresentando atividade inibitória da agregação plaquetária e em que não há atividade hemolítica in vitro foi identificado. Três (3) extratos (4 % do total de extratos testados), todos obtidos a partir de Elaeoluma nuda (Sapotaceae), apresentaram atividade hemolítica. Nenhuma das espécies vegetais nesse estudo tem uso medicinal conhecido. Assim, esses dados servem de linha base ou mínimas das atividades antiplaquetária e hemolítica (e utilidade potencial) de plantas da floresta Amazônica. Finalmente, em geral, esses dados são os primeiros disponíveis sobre ação hemolítica e inibição da agregação plaquetária dos gêneros representados por essas espécies de plantas