Innovative experiences in teaching conservation. Involving communities’ interests on preservationtopics by fast investigations and social media dissemination

Since 2019, the authors carried out a didactical experience trough the Preservation Studio workshop in the historical center of Vimercate, a town in the north east area of Milan, implementing a convention agreement between the Municipality and the Atheneum. The convention was arranged in order to set the relationship between the three academic courses of the Politecnico di Milano and the administration of Vimercate, supporting the teaching staff by providing ac- cessibility to various services and some public properties located in the city-cen- ter. Thanks to this kind of agreement, the courses could be supported in their activities by document centers, public associations and the members of the local community, while the teaching staff offered a constant sharing of the main activ- ities by social media and periodical disseminations through public lectures. After maturing several years of didactical workshops on the main buildings of the his- torical center of Vimercate, this paper shows the results collected with the stu- dios: the active class strategies, the on-site survey campaigns, the evolution of the results observed by year after year inspections, ND testing activities and local community involvement. The impact coming from the researches developed by the preservation classes and specific in depth studies realized by graduation thesis showed an increasing participation of the community to the topics connected to the city center: from conservation policies to future uses, historical buildings reached the attention of the people through the development of a new sensibility and perception of new values associated to the local architectural heritage

Assessment of Poly(ADP-ribose) Polymerase1 (PARP1) expression and activity in cells purified from blood and milk of dairy cattle

Poly(ADP-ribosyl)ation (PAR) is a post-translational protein modification catalysed by enzyme member of the poly(ADP-ribose) polymerases (PARPs) family. The activation of several PARPs is triggered by DNA strand breakage and the main PARP enzyme involved in this process is PARP1. Besides its involvement in DNA repair, PARP1 is involved in several cellular processes including transcription, epigenetics, chromatin re-modelling as well as in the maintenance of genomic stability. Moreover, several studies in human and animal models showed PARP1 activation in various inflammatory disorders. The aims of the study were (1) to characterize PARP1 expression in bovine peripheral blood mononuclear cells (PBMC) and (2) to evaluate PAR levels as a potential inflammatory marker in cells isolated from blood and milk samples following different types of infection, including mastitis. Our results show that (i) bovine PBMC express PARP1; (ii) lymphocytes exhibit higher expression of PARP1 than monocytes; (iii) PARP1 and PAR levels were higher in circulating PBMCs of infected cows; (iv) PAR levels were higher in cells isolated from milk with higher Somatic Cell Counts (SCC > 100,000 cells/mL) than in cells from milk with low SCCs. In conclusion, these findings suggest that PARP1 is activated during mastitis, which may prove to be a useful biomarker of mastitis

Structure of cattle, sheep, goat and buffalo populations using single nucleotide polymorphisms in genes affecting lipid metabolism

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THE MENSIOCHRONOLOGY ANALYSIS SUPPORTED BY NEW ADVANCED SURVEY TECHNIQUES: FIELD TESTS IN MILANESE AREA

Mensiochronology of brickwork, even in areas where reference curves have been constructed and well tested, requires precise measurements and a representative amount of data due to the many factors affecting the reliability of the measurements, such as the defects caused by the time and the environment that changed the geometry of the bricks (deformation or lack of material along the edges), as well as the operators’ skills. Sometimes, the number of measurable bricks is limited in a wall, or in a stratigraphic unit. Furthermore, if a scaffolding is not available, the analysis is concentrated only on the lower courses of the bricks, being not possible to directly measure the bricks of the higher levels. In order to implement the number of bricks taken into account for this study, a comparison from direct measurements and indirect measurements is here proposed.The aim of this paper is to explore the applications of photogrammetry for undertaking brick measurements for chronological dating: its advantage and drawbacks. Using high-resolution digital rectified pictures, the masonry texture of some well-documented building prospects was scaled into vector graphic software for recording the measures of the bricks. The results presented here by the authors are an attempt for validating this method for future applications. In detail, three case studies are analysed in the historic centre of Vimercate (Italy) testing the effectiveness of the presented method on dated buildings that display diverse features, including the presence of reused bricks, possibly coming from dismantled pre-existing structures. The results proved that the proposed geomatics method entails an accuracy that does not affect the usability of data for the investigation of buildings and the material culture inbuilt.</p

Casein SNP in Norwegian goats: additive and dominance effects on milk composition and quality

<p>Abstract</p> <p>Background</p> <p>The four casein proteins in goat milk are encoded by four closely linked casein loci (<it>CSN1S1</it>, <it>CSN2</it>, <it>CSN1S2 </it>and <it>CSN3</it>) within 250 kb on caprine chromosome 6. A deletion in exon 12 of <it>CSN1S1</it>, so far reported only in Norwegian goats, has been found at high frequency (0.73). Such a high frequency is difficult to explain because the national breeding goal selects against the variant's effect.</p> <p>Methods</p> <p>In this study, 575 goats were genotyped for 38 Single Nucleotide Polymorphisms (SNP) located within the four casein genes. Milk production records of these goats were obtained from the Norwegian Dairy Goat Control. Test-day mixed models with additive and dominance fixed effects of single SNP were fitted in a model including polygenic effects.</p> <p>Results</p> <p>Significant additive effects of single SNP within <it>CSN1S1 </it>and <it>CSN3 </it>were found for fat % and protein %, milk yield and milk taste. The allele with the deletion showed additive and dominance effects on protein % and fat %, and overdominance effects on milk quantity (kg) and lactose %. At its current frequency, the observed dominance (overdominance) effects of the deletion allele reduced its substitution effect (and additive genetic variance available for selection) in the population substantially.</p> <p>Conclusions</p> <p>The selection pressure of conventional breeding on the allele with the deletion is limited due to the observed dominance (overdominance) effects. Inclusion of molecular information in the national breeding scheme will reduce the frequency of this deletion in the population.</p