Association between Body Size and Mortality in Later Life

The rising prevalence of obesity in the United States has focused attention on the health consequences of excess weight. Obesity is linked to many of the major causes of death in the United States, including heart disease, some types of cancer, strokes, diabetes, and atherosclerosis. However, the effects of body size on mortality and health among the elderly are much less clear. This paper extends our current understanding of the relationship between body size and mortality by using two nationally representative, longitudinal datasets of individuals at older ages. These analyses indicate that obesity may not contribute greatly to increased mortality risks after age 70. In fact, obesity may be somewhat protective and lower the risk of death. At younger ages, however, the effects of obesity tend to operate in the opposite direction. Women especially appear to be at greater risk of death in their 50s and 60s if they are overweight

Dating Around: Investigating Gender Differences in First Date Behavior Using Self‐Report and Content Analyses from Netflix

In the current studies, we used qualitative, quantitative, and content analysis to understand which frst date behaviors individuals perceive as critical for landing second dates. In Study 1 (N=100), men and women reported tactics or behaviors that they believed someone of their gender would perform on a successful frst date. We observed that men and women perceive similar behaviors as important to engage in on successful frst dates. Study 2 (N=131) had men and women rate the perceived efectiveness of these tactics. When the behaviors are aggregated, men perceive women who engage in “Involvement” behaviors as better at obtaining sec- ond dates. In contrast, women perceive men who engage in “Etiquette” behaviors as more likely to land second dates. In Study 3, we coded the Netfix show, Dat- ing Around, using the tactics from Study 1 to examine if engaging in these tactics more frequently predicted having a successful date. Results revealed that receiving a second date was not related to an individual’s engagement in these tactics. This research is the frst to attempt to empirically delineate tactics that occur on frst dates and lead to second dates for men and women

In utero exposure to cigarette smoking, environmental tobacco smoke and reproductive hormones in US girls approaching puberty

BACKGROUND/AIMS: Evidence is unclear whether prenatal smoking affects age at menarche and pubertal development, and its impact upon hormones has not been well studied. We aim to identify potential pathways through which prenatal smoking and environmental tobacco smoke (ETS) affect reproductive hormones in girls approaching puberty. METHODS: We examined the association between prenatal smoking, current ETS and luteinizing hormone (LH) and inhibin B (InB) in 6- to 11-year-old girls in the 3rd National Health and Nutrition Examination Survey, 1988-1994. Parents/guardians completed interviewer-assisted questionnaires on health and demographics at the time of physical examination. Residual blood samples were analyzed for reproductive hormones in 2008. RESULTS: Of 660 girls, 19 and 39% were exposed to prenatal smoke and current ETS, respectively. Accounting for multiple pathways in structural equation models, prenatally exposed girls had significantly lower LH (β = -0.205 log-mIU/ml, p < 0.0001) and InB (β = -0.162, log-pg/ml, p < 0.0001). Prenatal smoking also influenced LH positively and InB negatively indirectly through BMI-for-age. ETS was positively associated with LH, but not with InB. CONCLUSION: Exposure to maternal smoking may disrupt reproductive development manifesting in altered hormone levels near puberty

L30A Mutation of Phospholemman Mimics Effects of Cardiac Glycosides in Isolated Cardiomyocytes

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biochemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.biochem.6b00633To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM–Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to NKA for several PLM mutants compared to that of the wild type (WT), including L27A, L30A, and I32A. In isolated cardiomyocytes, overexpression of WT PLM increased the amplitude of the Ca2+ transient compared to the GFP control. The Ca2+ transient amplitude was further increased by L30A PLM overexpression. The L30A mutation also delayed Ca2+ extrusion and increased the duration of cardiomyocyte contraction. This mimics aspects of the effect of cardiac glycosides, which are known to increase contractility through inhibition of NKA. No significant differences between WT and L30A PLM-expressing myocytes were observed after treatment with isoproterenol, suggesting that the superinhibitory effects of L30A are reversible with β-adrenergic stimulation. We also observed a decrease in the extent of PLM tetramerization with L30A compared to WT using FRET, suggesting that L30 is an important residue for mediating PLM–PLM binding. Molecular dynamics simulations revealed that the potential energy of the L30A tetramer is greater than that of the WT, and that the transmembrane α helix is distorted by the mutation. The results identify PLM residue L30 as an important determinant of PLM tetramerization and of functional inhibition of NKA by PLM.Peer reviewe