86 research outputs found
Genetic integrity of the Dark European honey bee (Apis mellifera mellifera) from protected populations: a genome-wide assessment using SNPs and mtDNA sequence data
The recognition that the Dark European honey bee, Apis mellifera mellifera, is increasingly threatened in its native range has led to the establishment of conservation programmes and protected areas throughout western Europe. Previous molecular surveys showed that, despite management strategies to preserve the genetic integrity of A. m. mellifera, protected populations had a measurable component of their gene pool derived from commercial C-lineage honey bees. Here we used both sequence data from the tRNAleu-cox2 intergenic mtDNA region and a genome-wide scan, with over 1183 single nucleotide polymorphisms (SNPs), to assess genetic diversity and introgression levels in several protected populations of A. m. mellifera, which were then compared with samples collected from unprotected populations. MtDNA analysis of the protected populations revealed a single colony bearing a foreign haplotype, whereas SNPs showed varying levels of introgression ranging from virtually zero in Norway to about 14% in Denmark. Introgression overall was higher in unprotected (30%) than in protected populations (8%), and is reflected in larger SNP diversity levels of the former, although opposite diversity levels were observed for mtDNA. These results suggest that, despite controlled breeding, some protected populations still require adjustments to the management strategies to further purge foreign alleles, which can be identified by SNPs.Pint
The mitochondrial genome of the stingless bee Melipona bicolor (Hymenoptera, Apidae, Meliponini): sequence, gene organization and a unique tRNA translocation event conserved across the tribe Meliponini
High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool
The natural distribution of the honeybee (Apis mellifera L.) has been changed by humans in recent
decades to such an extent that the formerly widest-spread European subspecies, Apis mellifera
mellifera, is threatened by extinction through introgression from highly divergent commercial strains
in large tracts of its range. Conservation efforts for A. m. mellifera are underway in multiple European
countries requiring reliable and cost-efficient molecular tools to identify purebred colonies. Here, we
developed four ancestry-informative SNP assays for high sample throughput genotyping using the
iPLEX Mass Array system. Our customized assays were tested on DNA from individual and pooled,
haploid and diploid honeybee samples extracted from different tissues using a diverse range of
protocols. The assays had a high genotyping success rate and yielded accurate genotypes. Performance
assessed against whole-genome data showed that individual assays behaved well, although the
most accurate introgression estimates were obtained for the four assays combined (117 SNPs).
The best compromise between accuracy and genotyping costs was achieved when combining two
assays (62 SNPs). We provide a ready-to-use cost-effective tool for accurate molecular identification
and estimation oinfo:eu-repo/semantics/publishedVersio
Genetic structure of drone congregation areas of Africanized honeybees in southern Brazil
As yet, certain aspects of the Africanization process are not well understood, for example, the reproductive behavior of African and European honeybees and how the first Africanized swarms were formed and spread. Drone congregation areas (DCAs) are the ideal place to study honeybee reproduction under natural conditions since hundreds of drones from various colonies gather together in the same geographical area for mating. In the present study, we assessed the genetic structure of seven drone congregations and four commercial European-derived and Africanized apiaries in southern Brazil, employing seven microsatellite loci for this purpose. We also estimated the number of mother-colonies that drones of a specific DCA originated from. Pairwise comparison failed to reveal any population sub-structuring among the DCAs, thus indicating low mutual genetic differentiation. We also observed high genetic similarity between colonies of commercial apiaries and DCAs, besides a slight contribution from a European-derived apiary to a DCA formed nearby. Africanized DCAs seem to have a somewhat different genetic structure when compared to the European
Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder
A survivor population of wild colonies of European honeybees in the northeastern United States: investigating its genetic structure
The physical, insemination, and reproductive quality of honey bee queens (Apis mellifera L.)
Variabilité de l'ADN mitochondrial de l'abeille domestique implications phylogénétiques
* Inra Poitou-Charentes, ERIST, Route de Saintes BP 6, 86600 Lusignan (FRA) Diffusion du document : Inra Poitou-Charentes, ERIST, Route de Saintes BP 6, 86600 Lusignan (FRA) DiplĂŽme : Dr. d'Universit
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