Identificação e caracterização de microssatélites provenientes de sequências expressas do projeto brasileiro de genoma café.

Dentre os marcadores genéticos disponíveis, os microssatélites ou SSRs (Simple Sequence Repeats) têm sido os escolhidos para diferentes estudos genéticos por apresentarem características que agregam simplicidade técnica, grande poder de resolução e alto nível de polimorfismo. Com a disponibilidade das seqüências ESTs (Expressed Sequence Tags), geradas pelo Projeto Brasileiro do Genoma Café, surgiu a oportunidade de desenvolver esses marcadores, de forma direta e eficiente, por meio de análise eletrônica. Visando o desenvolvimento futuro de marcadores SSRs para café, nesse trabalho foram identificados e caracterizados microssatélites nas seqüências expressas do genoma do cafeeiro. A mineração dos dados foi realizada utilizando-se todas as combinações de di-, tri- e tetranucleotídeos formadores dos microssatélites, perfazendo um total de 46 combinações (quatro de dinucleotídeos, 10 de trinucleotídeos e 32 de tetranucleotídeos). Foram considerados os microssatélites perfeitos e com tamanho mínimo de 12 pares de bases. Do total de 130.792 ESTs proveniente de C. arabica foram identificadas 37.826 contendo microssatélites, que após a clusterização resultou em 24.031 EST-SSR. Dentre estas, 45,11% apresentaram tetranucleotídeos, 36,67% trinucleotídeos e 18,23% dinucleotídeos. As unidades repetitivas mais abundantes dentro de cada categoria de EST-SSR foram (AG)n encontrada em 57,08% das sequências contendo dinucleotídeos, (AGG)n com 30,79% dos trinucleotídeos e (AGGG)n com 33,24% dos tetranucleotídeos. A grande quantidade de SSRs identificada nas seqüências transcritas do genoma do cafeeiro demonstra que essas são fontes valiosas para o desenvolvimento dos marcadores moleculares EST-SSRs

On the variability of HD 170699 - a possible COROT target

We present the analysis of the variability of HD 170699, a COROT star showing the characteristics of a non evolutionary Delta Scuti star with high rotational velocity. There is a clear period of 10.45 c/d with 5.29 mmag amplitude in the y filter. From the data, it can be seen that the star shows multi-periodicity and it is necessary to add more frequencies to adjust the observationsComment: To appear in RevMexAA(SC) in Proceedings of XII Reunion Regional Latinoamericana de la UAI held in Isla Margarita, Venezuela, October 22-26, 200

An analog of Heisenberg uncertainty relation in prequantum classical field theory

Prequantum classical statistical field theory (PCSFT) is a model which provides a possibility to represent averages of quantum observables, including correlations of observables on subsystems of a composite system, as averages with respect to fluctuations of classical random fields. PCSFT is a classical model of the wave type. For example, "electron" is described by electronic field. In contrast to QM, this field is a real physical field and not a field of probabilities. An important point is that the prequantum field of e.g. electron contains the irreducible contribution of the background field, vacuum fluctuations. In principle, the traditional QM-formalism can be considered as a special regularization procedure: subtraction of averages with respect to vacuum fluctuations. In this paper we derive a classical analog of the Heisenberg-Robertson inequality for dispersions of functionals of classical (prequantum) fields. PCSFT Robertson-like inequality provides a restriction on the product of classical dispersions. However, this restriction is not so rigid as in QM. The quantum dispersion corresponds to the difference between e.g. the electron field dispersion and the dispersion of vacuum fluctuations. Classical Robertson-like inequality contains these differences. Hence, it does not imply such a rigid estimate from below for dispersions as it was done in QM

Brownian Entanglement

We show that for two classical brownian particles there exists an analog of continuous-variable quantum entanglement: The common probability distribution of the two coordinates and the corresponding coarse-grained velocities cannot be prepared via mixing of any factorized distributions referring to the two particles in separate. This is possible for particles which interacted in the past, but do not interact in the present. Three factors are crucial for the effect: 1) separation of time-scales of coordinate and momentum which motivates the definition of coarse-grained velocities; 2) the resulting uncertainty relations between the coordinate of the brownian particle and the change of its coarse-grained velocity; 3) the fact that the coarse-grained velocity, though pertaining to a single brownian particle, is defined on a common context of two particles. The brownian entanglement is a consequence of a coarse-grained description and disappears for a finer resolution of the brownian motion. We discuss possibilities of its experimental realizations in examples of macroscopic brownian motion.Comment: 18 pages, no figure

Preliminary results from the STEPHI2009 campaign on the open cluster NGC 1817

We present preliminary observational results of the multi-site STEPHI campaign on the cluster NGC 1817. The three observatories involved are San Pedro Martir (Mexico), Xing Long (China) and the Observatorio del Teide (Spain) - giving an ideal combination to maximise the duty cycle. The cluster has 12 known delta Scuti stars and at least two detached eclipsing binary systems. This combination of characteristics is ideal for extracting information about global parameters of the targets, which will in turn impose strict constraints on the stellar models. From an initial comparison with stellar models using the known fundamental parameters, and just the observed pulsation frequencies and measured effective temperatures, it appears that a lower value of initial helium mass fraction will most likely explain the observations of these stars.Comment: 4 pages, proceedings from HELAS IV meeting 2010, Lanzarot

Jets, knots and tails in planetary nebulae: NGC 3918, K 1-2 and Wray 17-1

We analyze optical images and high-resolution, long-slit spectra of three planetary nebulae which possess collimated, low-ionization features. NGC 3918 is composed of an inner, spindle-shaped shell mildly inclined with respect to the plane of the sky. Departing from the polar regions of this shell, we find a two-sided jet expanding with velocities which increase linearly with distance from 50 to 100 km/s. The jet is probably coeval with the inner shell (with the age of approximately 1000 D yr, where D is the distance in kpc), suggesting that its formation should be ascribed to the same dynamical processes which also shaped the main nebula, and not to a more recent mass loss episode. We discuss the formation of the aspherical shell and jet in the light of current hydrodynamical and magnetohydrodynamical theories. K 1-2 is a planetary nebula with a close binary nucleus which shows a collimated string of knots embedded in a diffuse, elliptical shell. The knots expand with a velocity similar to that of the elliptical nebula (25 km/s), except for an extended tail located out of the main nebula, which linearly accelerates up to 45 km/s. We estimate an inclination on the line of the sight of 40 degres for the string of knots; once the orientation of the orbit is also determined, this information will allow us to test the prediction of current theories of the occurrence of polar jets from close binary systems. Wray 17-1 has a complex morphology, showing two pairs of low-ionization structures located in almost perpendicular directions from the central star, and embedded in a large, diffuse nebula. The two pairs show notable similarities and differences, and their origin is very puzzling.Comment: 20 pages plus 10 figures. ApJ recently published (ApJ 523, 721 (1999)

Deficiency of Kruppel-Like Factor KLF4 in Myeloid-Derived Suppressor Cells Inhibits Tumor Pulmonary Metastasis in Mice Accompanied by Decreased Fibrocytes

The importance of immunosuppressive myeloid-derived suppressor cells (MDSCs) bearing monocyte markers in tumor metastasis has been well established. Recently, it was reported that these cells possess phenotypic plasticity and differentiate into fibrocytes, very distinct cells that are precursors of tumorigenic myofibroblasts. However, the importance of this transdifferentiation in tumor metastasis has not been explored. Here, we describe the role of MDSC-derived fibrocytes in tumor metastasis that is regulated by Kruppel-like factor 4 (KLF4), a transcription factor that is critical to monocyte differentiation and to promotion of cancer development. Using mouse metastasis models of melanoma and breast cancer, we found that KLF4 knockout was associated with significantly reduced pulmonary metastasis, which was accompanied by decreased populations of MDSCs, fibrocytes and myofibroblasts in the lung. Cause-effect studies by adoptive transfer revealed that KLF4 deficiency in MDSCs led to significantly reduced lung metastasis that was associated with fewer MDSC-derived fibrocytes and myofibroblasts. Mechanistically, KLF4 deficiency significantly compromised the generation of fibrocytes from MDSCs in vitro. During this process, KLF4 expression levels were tightly linked with those of fibroblast-specific protein-1 (FSP-1), deficiency of which resulted in no metastasis in mice as has been previously reported. In addition, KLF4 bound directly to the FSP-1 promoter as determined by chromatin immunoprecipitation and overexpression of KLF4 increased the FSP-1 promoter activities. Taken together, our results suggest that MDSCs not only execute their immunosuppressive function to promote metastatic seeding as reported before, but also boost metastatic tumor growth after they adopt a fibrocyte fate. Therefore, KLF4-mediated fibrocyte generation from MDSCs may represent a novel mechanism of MDSCs contributing to tumor metastasis and supports the feasibility of inhibiting KLF4 or FSP-1 to prevent tumor metastasis