Revised reference model for nitric acid

A nearly global set of data on the nitric acid distribution was obtained for seven months by the Limb Infrared Monitor of the Stratosphere (LIMS) experiment on the Nimbus 7 spacecraft. The evaluation of the accuracy, precision, and resolution of these data is described, and a description of the major features of the nitric acid distributions is presented. The zonal mean for nitric acid is distributed in a stratospheric layer that peaks near 30 mb, with the largest mixing ratios occurring in polar regions, especially in winter

Chandra Observation of Abell 2065: An Unequal Mass Merger?

We present an analysis of a 41 ks Chandra observation of the merging cluster Abell 2065 with the ACIS-I detector. Previous observations with ROSAT and ASCA provided evidence for an ongoing merger, but also suggested that there were two surviving cooling cores, which were associated with the two cD galaxies in the center of the cluster. The Chandra observation reveals only one X-ray surface brightness peak, which is associated with the more luminous, southern cD galaxy. The gas related with that peak is cool and displaced slightly from the position of the cD. The data suggest that this cool material has formed a cold front. On the other hand, in the higher spatial resolution Chandra image, the second feature to the north is not associated with the northern cD; rather, it appears to be a trail of gas behind the main cD. We argue that only one of the two cooling cores has survived the merger, although it is possible that the northern cD may not have possessed a cool core prior to the merger. We use the cool core survival to constrain the kinematics of the merger and we find an upper limit of ~< 1900 km/s for the merger relative velocity. A surface brightness discontinuity is found at ~140 kpc from the southern cD; the Mach number for this feature is ${\cal M} = 1.66^{+0.24}_{-0.32}$, although its nature (shock or cold front) is not clear from the data. We argue that Abell 2065 is an example of an unequal mass merger. The more massive southern cluster has driven a shock into the ICM of the infalling northern cluster, which has disrupted the cool core of the latter, if one existed originally. We estimate that core crossing occurred a few hundred Myr ago, probably for the first time.Comment: 15 pages, 10 figures, ApJ in pres

Using food intake records to estimate compliance with the Eatwell plate dietary guidelines

This work was supported by the Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division. The original studies, from which the current data were taken, were funded by the Food Standards Agency, UK, and the Biscuit, Cake, Chocolate and Confectionery Association, London, UK.Peer reviewedPostprin

The Toxoplasma gondii plastid replication and repair enzyme complex, PREX

A plastid-like organelle, the apicoplast, is essential to the majority of medically and veterinary important apicomplexan protozoa including Toxoplasma gondii and Plasmodium. The apicoplast contains multiple copies of a 35 kb genome, the replication of which is dependent upon nuclear-encoded proteins that are imported into the organelle. In P. falciparum an unusual multi-functional gene, pfprex, was previously identified and inferred to encode a protein with DNA primase, DNA helicase and DNA polymerase activities. Herein, we report the presence of a prex orthologue in T. gondii. The protein is predicted to have a bi-partite apicoplast targeting sequence similar to that demonstrated on the PfPREX polypeptide, capable of delivering marker proteins to the apicoplast. Unlike the P. falciparum gene that is devoid of introns, the T. gondii prex gene carries 19 introns, which are spliced to produce a contiguous mRNA. Bacterial expression of the polymerase domain reveals the protein to be active. Consistent with the reported absence of a plastid in Cryptosporidium species, in silico analysis of their genomes failed to demonstrate an orthologue of prex. These studies indicate that prex is conserved across the plastid-bearing apicomplexans and may play an important role in the replication of the plastid genome

Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions

Phenotypic robustness is evidenced when single-gene mutations do not result in an obvious phenotype. It has been suggested that such phenotypic stability results from 'buffering' activities of homologous genes as well as non-homologous genes acting in parallel pathways. One approach to characterizing mechanisms of phenotypic robustness is to identify genetic interactions, specifically, double mutants where buffering is compromised. To identify interactions among genes implicated in posterior patterning of the Caenorhabditis elegans embryo, we measured synthetic lethality following RNA interference of 22 genes in 15 mutant strains. A pair of homologous T-box transcription factors (tbx-8 and tbx-9) is found to interact in both C. elegans and C. briggsae, indicating that their compensatory function is conserved. Furthermore, a muscle module is defined by transitive interactions between the MyoD homolog hlh-1, another basic helix-loop-helix transcription factor, hnd-1, and the MADS-box transcription factor unc-120. Genetic interactions within a homologous set of genes involved in vertebrate myogenesis indicate broad conservation of the muscle module and suggest that other genetic modules identified in C. elegans will be conserved

Heating of the Intracluster Gas in the Triangulum Australis Cluster

ASCA and ROSAT X-ray data are used to obtain two-dimensional maps of the gas temperature, pressure and specific entropy in the Triangulum Australis cluster of galaxies. We find that this hot (T_e=10.3+-0.8 keV) system probably has a temperature peak (T_e>12 keV) at the cluster core, which approximately corresponds to the adiabatic relation. An underdense gas sector, found in the ROSAT cluster image eastward of the core, has a higher temperature than average at that radius. At this higher temperature, the gas pressure in this region is equal to that of the rest of the cluster at the same radius, but the specific entropy of this gas is significantly higher (although the temperature difference itself is only marginally significant). We speculate that the existence of this region of underdense high-entropy gas, as well as the adiabatic central temperature peak, indicate recent or ongoing heating of the intergalactic medium in this cluster. The most probable source of such heating is a subcluster merger, for which the hydrodynamic simulations predict a qualitatively similar temperature structure. We point out that entropy maps can provide a physically meaningful way of diagnosing merging clusters and comparing the predictions of merger simulations to the data.Comment: Text revised to give more technical details and clarify discussion. Accepted for ApJ Letters. Latex, 5 pages incl. figures, macros include

What features of a nutrition resource are important to adolescents of a low socioeconomic status?

Acknowledgements: The authors would like to thank Dr Mary Bellizzi, Dr Julia Allan, Julia Clark, Karen Tosh, Dr Heather Morgan and Neil Hendry for their contributions during the development of structured interview questions. We would like to thank Hannah Clews for her continued support during the Streetsport sessions, both prior to and during data collection. Additionally, we would like to thank the staff at Streetsport for their feedback on structured interview questions. Financial support: This work was supported by the University of Aberdeen.Peer reviewedPostprin

Optical binding in nanoparticle assembly: Potential energy landscapes

Optical binding is an optomechanical effect exhibited by systems of micro- and nanoparticles, suitably irradiated with off-resonance laser light. Physically distinct from standing-wave and other forms of holographic optical traps, the phenomenon arises as a result of an interparticle coupling with individual radiation modes, leading to optically induced modifications to Casmir-Polder interactions. To better understand how this mechanism leads to the observed assemblies and formation of patterns in nanoparticles, we develop a theory in terms of optically induced energy landscapes exhibiting the three-dimensional form of the potential energy field. It is shown in detail that the positioning and magnitude of local energy maxima and minima depend on the configuration of each particle pair, with regards to the polarization and wave vector of the laser light. The analysis reveals how the positioning of local minima determines the energetically most favorable locations for the addition of a third particle to each equilibrium pair. It is also demonstrated how the result of such an addition subtly modifies the energy landscape that will, in turn, determine the optimum location for further particle additions. As such, this development represents a rigorous and general formulation of the theory, paving the way toward full comprehension of nanoparticle assembly based on optical binding

Raman scattering mediated by neighboring molecules

Raman scattering is most commonly associated with a change in vibrational state within individual molecules, the corresponding frequency shift in the scattered light affording a key way of identifying material structures. In theories where both matter and light are treated quantum mechanically, the fundamental scattering process is represented as the concurrent annihilation of a photon from one radiation mode and creation of another in a different mode. Developing this quantum electrodynamical formulation, the focus of the present work is on the spectroscopic consequences of electrodynamic coupling between neighboring molecules or other kinds of optical center. To encompass these nanoscale interactions, through which the molecular states evolve under the dual influence of the input light and local fields, this work identifies and determines two major mechanisms for each of which different selection rules apply. The constituent optical centers are considered to be chemically different and held in a fixed orientation with respect to each other, either as two components of a larger molecule or a molecular assembly that can undergo free rotation in a fluid medium or as parts of a larger, solid material. The two centers are considered to be separated beyond wavefunction overlap but close enough together to fall within an optical near-field limit, which leads to high inverse power dependences on their local separation. In this investigation, individual centers undergo a Stokes transition, whilst each neighbor of a different species remains in its original electronic and vibrational state. Analogous principles are applicable for the anti-Stokes case. The analysis concludes by considering the experimental consequences of applying this spectroscopic interpretation to fluid media; explicitly, the selection rules and the impact of pressure on the radiant intensity of this process

A retarded coupling approach to intermolecular interactions

A wide range of physical phenomena such as optical binding and resonance energy transfer involve electronic coupling between adjacent molecules. A quantum electrodynamical description of these intermolecular interactions reveals the presence of retardation effects. The clarity of the procedure associated with the construction of the quantum amplitudes and the precision of the ensuing results for observable energies and rates are widely acknowledged. However, the length and complexity of the derivations involved in such quantum electrodynamical descriptions increase rapidly with the order of the process under study. Whether through the use of time-ordering approaches, or the more expedient state-sequence method, time-consuming calculations cannot usually be bypassed. A simple and succinct method is now presented, which provides for a direct and still entirely rigorous determination of the quantum electrodynamical amplitudes for processes of arbitrarily high order. Using the approach, new results for optical binding in two- and three-particle systems are secured and discussed