On output feedback nonlinear model predictive control using high gain observers for a class of systems

In recent years, nonlinear model predictive control schemes have been derived that guarantee stability of the closed loop under the assumption of full state information. However, only limited advances have been made with respect to output feedback in connection to nonlinear predictive control. Most of the existing approaches for output feedback nonlinear model predictive control do only guarantee local stability. Here we consider the combination of stabilizing instantaneous NMPC schemes with high gain observers. For a special MIMO system class we show that the closed loop is asymptotically stable, and that the output feedback NMPC scheme recovers the performance of the state feedback in the sense that the region of attraction and the trajectories of the state feedback scheme are recovered for a high gain observer with large enough gain and thus leading to semi-global/non-local results

AN ECONOMIC ANALYSIS OF GENETIC INFORMATION: LEPTIN GENOTYPING IN FED CATTLE

The use of genetic knowledge is widespread in crop production but is just recently being utilized in livestock production. This study investigates the economic value to feedlots of a polymorphism in the bovine leptin gene. Previous studies indicate that this polymorphism is associated with fat deposition. Since fed cattle are often priced on a grid that considers both yield and quality grades, fat deposition is an important factor in the value and profitability of fed cattle. Using data from 590 crossbred steers and heifers, we estimate growth curves for relevant biological traits, both with and without genotypic information. Using the resulting functions, we then simulate carcass traits to various days-on-feed and compute the associated profit under three price grids. Maximum profits are determined in an unconstrained profit maximization model and in a model that constrains cattle to be marketed in 45-head "potloads." Results indicate that leptin genotypic knowledge has little impact on optimal days-on-feed but may play a role in valuing feeder cattle. The differences in value of cattle varied by as much as $37 per head between genotypes.genetics, leptin genotype, beef cattle, value of information, Livestock Production/Industries,

Concurrent Verbal Encouragement and Wingate Anaerobic Cycle Test Performance in Females: Athletes vs. Non-Athletes

International Journal of Exercise Science 5(3) : 239-244, 2012. To examine the effect of concurrent verbal encouragement on the performance of the WAnT in female athletes vs. female non-athletes. College-age female subjects were recruited where ten of the subjects were intercollegiate athletes (ATH, n1=10) and the nine were non-athletes (NON, n2=9). The WAnT was novel to all subjects and the subjects were blinded to the study’s purpose. Prior to the experimental trials, subjects were measured for body composition and performed a familiarity WAnT trial without verbal encouragement. Subjects then performed the WAnT twice, once with concurrent verbal encouragement (VE) and once without (NVE), in a balanced cross-over design. Peak (PP) and mean power (MP), and total work (TW) were compared between ATH and NON across VE and NVE using an ANOVA (1 between, 1 within), α=0.05. ATH and NON did not differ (p\u3e0.05) in age or body composition with the exception of fat-free mass which differed significantly (ATH=53.7±6.6, NON=46.1±5.7 kg) (p\u3c0.05). A significant (p\u3c0.05) main effect for ATH/NON was observed where ATH outperformed NON when pooled across VE/NVE trials for PP (ATH=13.0±1.4, NON=11.3±1.7 W·kg-1), MP (ATH=7.7±1.1, NON=6.7±0.9 W·kg-1) and TW (ATH=232±35, NON=201±26 J·kg-1). When pooled across all subjects (ATH and NON), the VE/NVE trials did not differ (p\u3e0.05) for PP (VE=12.4±1.7, NVE=12.0±1.9 W·kg-1), MP (VE=7.3±1.1, NVE=7.2±1.2 W·kg-1) and TW (VE=219±33, NVE=215±35 J·kg-1). The ATH/NON interaction with VE/NVE was not significant (p\u3e0.05). Concurrent verbal encouragement does not affect performance on the WAnT in females, nor does it affect WAnT performance in female athletes and non-athletes differently

Concurrent Verbal Encouragement and Wingate Anaerobic Cycle Test Performance in Females: Athletes vs. Non-Athletes

Previous research has shown that concurrent verbal encouragement improves performance on the Wingate Anaerobic Cycle Test (WACT), a 30-s supramaximal exercise protocol, in male non-athletes. This effect has not previously been examined in athletes or females. Consistently, athletes and females display a more self-determined motivational profile than non-athletes and males, suggesting verbal encouragement to be of lesser consequence to athletes and females. PURPOSE: To examine the effect of concurrent verbal encouragement on the performance of the WACT in female athletes vs. female non-athletes. METHODS: Nineteen college-age female subjects were recruited where ten of the subjects were active intercollegiate athletes (ATH, n1=10) and the remainder were non-athletes (NON, n2=9). The WACT was novel to all subjects and the subjects were blinded to the purpose of the study. Prior to participation in the experimental trials, subjects were measured for body composition and performed a familiarity WACT trial without verbal encouragement. Subjects then performed the WACT twice, once with concurrent verbal encouragement (VE) and once without (NVE), in a balanced cross-over design. All WACT trials were performed at least one week apart. Peak power (PP), mean power (MP), and total work (TW) were compared between ATH and NON across VE and NVE using an ANOVA (1 between, 1 within), α=0.05. RESULTS: ATH and NON did not differ significantly (p\u3e0.05) with regard to age or body composition with the exception of fat-free mass which differed significantly (ATH=53.7±6.6, NON=46.1±5.7 kg)(p\u3c0.05). As expected, a significant (p\u3c0.05) main effect for ATH/NON was observed where ATH outperformed NON when pooled across VE/NVE trials for PP (ATH=13.0±1.4, NON=11.3±1.7 W·kg-1), MP (ATH=7.7±1.1, NON=6.7±0.9 W·kg-1) and TW (ATH=232±35, NON=201±26 J·kg-1). However, when pooled across all subjects (ATH and NON), the VE/NVE trials did not differ significantly (p\u3e0.05) for PP (VE=12.4±1.7, NVE=12.0±1.9 W·kg-1), MP (VE=7.3±1.1, NVE=7.2±1.2 W·kg-1) and TW (VE=219±33, NVE=215±35 J·kg-1). Additionally, the ATH/NON interaction with VE/NVE was not significant (p\u3e0.05). CONCLUSION: Concurrent verbal encouragement does not affect performance on the WACT in females, nor does it affect WACT performance in female athletes and non-athletes differently

The recurrent atypical e8a2 BCR::ABL1 transcript with insertion of an inverted 55 base pair ABL1 intron 1b sequence: a detailed molecular analysis

Atypical BCR::ABL1 transcripts are found in approximately 2% of cases of chronic myeloid leukemia. It is important to detect them, since affected patients benefit from tyrosine kinase inhibitor therapy, similar to patients with typical BCR::ABL1 variants. In the rare e8a2 atypical BCR::ABL1 transcript two out-of-frame exons are fused, thus, interposed nucleotides are usually found at the fusion site to restore the reading frame. In approximately half of previously reported e8a2 BCR::ABL1 cases an inserted 55 bp sequence homologous to an inverted sequence from ABL1 intron 1b was detected. The generation of this recurrent transcript variant is not obvious. This work describes the molecular analysis of such an e8a2 BCR::ABL1 translocation from a CML patient. The genomic chromosomal breakpoint is identified, and the formation of this transcript variant is theoretically explained. The clinical course of the patient is reported, and recommendations are provided for the molecular analysis of future e8a2 BCR::ABL1 cases

Evaluation of the American-English Quality of Life in Short Stature Youth (QoLISSY) questionnaire in the United States.

BACKGROUND: The European Quality of Life in Short Stature Youth (QoLISSY) is a novel condition-specific instrument developed to assess health related quality of life (HrQoL) in children/adolescents with short stature from patient and parent perspectives. Study objective was to linguistically validate and psychometrically test the American-English version of the QoLISSY instrument. METHODS: Upon conversion of the British-English version to American-English, content validity and acceptance of the questionnaire were examined through focus group discussions with cognitive debriefing in 28 children/adolescents with growth hormone deficiency (GHD) or idiopathic short stature (ISS) and their parents. In the subsequent field test with 51 families and a re-test with 25 families the psychometric performance of the American-English version was examined and compared with the original European dataset. RESULTS: Pilot test results supported the suitability of the American-English version. Good internal consistency with Cronbach\u27s Alpha ranging from 0.84 to 0.97 and high test-re-test reliabilities were observed in the field test. The QoLISSY was able to detect significant differences according to the degree of short stature with higher HrQoL for taller children. Correlations with a generic HrQoL tool support the QoLISSY\u27s concurrent validity. The scale\u27s operating characteristics were comparable to the original European data. CONCLUSION: Results support that the QoLISSY American-English version is a psychometrically sound short stature-specific instrument to assess the patient- and parent- perceived impact of short stature. The QoLISSY instrument is fit for use in clinical studies and health services research in the American-English speaking population

Localization-associated immune phenotypes of clonally expanded tumor-infiltrating T cells and distribution of their target antigens in rectal cancer

The degree and type of T cell infiltration influence rectal cancer prognosis regardless of classical tumor staging. We asked whether clonal expansion and tumor infiltration are restricted to selected-phenotype T cells; which clones are accessible in peripheral blood; and what the spatial distribution of their target antigens is. From five rectal cancer patients, we isolated paired tumor-infiltrating T cells (TILs) and T cells from unaffected rectum mucosa (T(UM)) using 13-parameter FACS single cell index sorting. TCRαβ sequences, cytokine, and transcription factor expression were determined with single cell sequencing. TILs and T(UM) occupied distinct phenotype compartments and clonal expansion predominantly occurred within CD8(+) T cells. Expanded TIL clones identified by paired TCRαβ sequencing and exclusively detectable in the tumor showed characteristic PD-1 and TIM-3 expression. TCRβ repertoire sequencing identified 49 out of 149 expanded TIL clones circulating in peripheral blood and 41 (84%) of these were PD-1(-) TIM-3(-). To determine whether clonal expansion of predominantly tumor-infiltrating T cell clones was driven by antigens uniquely presented in tumor tissue, selected TCRs were reconstructed and incubated with cells isolated from corresponding tumor or unaffected mucosa. The majority of clones exclusively detected in the tumor recognized antigen at both sites. In summary, rectal cancer is infiltrated with expanded distinct-phenotype T cell clones that either i) predominantly infiltrate the tumor, ii) predominantly infiltrate the unaffected mucosa, or iii) overlap between tumor, unaffected mucosa, and peripheral blood. However, the target antigens of predominantly tumor-infiltrating TIL clones do not appear to be restricted to tumor tissue

A Performance-Based Scenario Methodology to Assess Collaborative Networks Business Model Dynamicity

[EN] In today's business marketplace many enterprises collaborate forming a collaborative network (CN) in order to achieve competitive and sustainable advantages. In this context, CNs should have not only well-defined business models but also mechanisms and tools that help them out to assess such business models as well as other CN operations at their early stages. Due to shorter life-cycles and to the current fierce competition such an evaluation should be made as quickly as possible and analyzing real data rather than based on opinions and subjective judgments. This paper presents the application of a methodology that allows such an assessment as well as the generation of business scenarios based on the performance of the CN. Then, it first defines the appropriate CN key performance indicators (KPIs), gathering data for a certain time-period; then, it applies multivariate techniques to this data, identifying relationships between the KPIs, and being able to build the timely evolution of the CN based on this data; next, it is able to design a business scenario based on the timely evolution that the CN should have according to its business models and operations results achieved so far. With all this additional information decision-makers could decide whether the CN's business models succeeded or not so far and what actions to take in order to achieve the future desirable scenario.This work has been developed within the research project called “Design of business scenarios to improve the efficiency and management of industrial supply chain” (reference GV/2013/045).Rodríguez Rodríguez, R.; Alfaro Saiz, JJ.; Verdecho Sáez, MJ. (2015). A Performance-Based Scenario Methodology to Assess Collaborative Networks Business Model Dynamicity. IFIP Advances in Information and Communication Technology. 463:511-517. https://doi.org/10.1007/978-3-319-24141-8_47S511517463Achtenhagen, L., Melin, L., Naldi, L.: Dynamics of business models – strategizing, critical capabilities and activities for sustained value creation. Long Range Plann. 46, 427–442 (2013)Chesbrough, H.: Business model innovation: opportunities and barriers. Long Range Plann. 43, 354–363 (2010)Chermack, T.J.: Studying scenario planning: theory, research, suggestions, and hypotheses. Technol. Forecast. Soc. Change 72, 59–73 (2005)Harries, C.: Correspondence to what? Coherence to what? What is good scenario-based decision making? Technol. Forecast. Soc. Change 70, 797–817 (2003)Gunasekaran, A., Patel, C., Tirtiroglu, E.: Performance measures and metrics in a supply chain environment. Int. J. Oper. Prod. Manage. 21, 71–87 (2001)Bullinger, H.J., Kühner, M., Hoof, A.V.: Analysing supply chain performance using a balanced measurement method. Int. J. Prod. Res. 40, 3533–3543 (2002)Folan, P., Browne, J.: Development of an extended enterprise performance measurement system. Prod. Plann. Control 16, 531–544 (2005)Fink, A., Marr, B., Siebe, A., Khule, J.-P.: The future scorecard: combining external and internal scenarios to create strategic foresight. Manage. Decis. 43, 360–381 (2005)Othman, R.: Enhancing the effectiveness of the balanced scorecard with scenario planning. Int. J. Prod. Perform. Manage. 57, 259–266 (2008)Rodriguez-Rodriguez, R., Saiz, J.J.A., Bas, A.O., Carot, J.M., Jabaloyes, J.M.: Building internal business scenarios based on real data from a performance measurement system. Technol. Forecast. Soc. Change 77, 50–62 (2010

Refining AML treatment: the role of genetics in response and resistance evaluation to new agents

The number of treatment options for acute myeloid leukemia (AML) has greatly increased since 2017. This development is paralleled by the broad implantation of genetic profiling as an integral part of clinical studies, enabling us to characterize mutation–response, mutation–non-response, or mutation–relapse patterns. The aim of this review is to provide a concise overview of the current state of knowledge with respect to newly approved AML treatment options and the association of response, relapse and resistance with genetic alterations. Specifically, we will highlight current genetic data regarding FLT3 inhibitors, IDH inhibitors, hypomethylating agents (HMA), the BCL-2 inhibitor venetoclax (VEN), the anti-CD33 antibody conjugate gemtuzumab ozogamicin (GO) and the liposomal dual drug CPX-351