59 research outputs found
Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI
The application of low-intensity electrical stimulation (LIES) to neural tissue increases neurochemical factors responsible for regeneration as nerve growth factor. Stem cell (SC) therapy for patients with Spinal cord injury (SCI) promote some increase functional improvement.ObjectiveInvestigate the electromyographic response in paraplegic dogs undergoing LIES and SC transplantation.Methods27 dogs paraplegics with SCI were divided into three groups with different types of therapy. GADSC: two SC transplants (n = 9); GLIES: LIES (n = 8); GCOMB: two SC transplants and LIES (n = 10). Adipose derived mesenchymal stem cells (ADSCs) were transplanted by lumbar puncture in the amount of 1.2 × 106 cells/50 μL. Acupuncture needles positioned in the interspinous space were used for stimulation. The electrical stimulation was applied with a mean voltage ∼30 mV and four consecutive modulated frequencies (5 Hz, 10 Hz, 15 Hz and 20 Hz) within 5 min each. The patients motor performance was evaluated before (Pre) the procedure and after 30 (Post30) and 60 (Post60) days, from electromyography root mean square (EMGRMS) registered with subcutaneous electrodes in the vastus lateralis muscle, while the animals were in quadrupedal position.ResultsAll three groups showed a significant intra-group increase of EMGRMS (Pre vs. Post30 or Pre vs. Post60). However, there were no statistically significant differences between Post30 and Post60. The inter-group test (GADSC X GLIES X GCOMB) did not present significance when compared the instants Pre (p = 0.34), Post30 (p = 0.78) and Post60 (p = 0.64).ConclusionSome dogs recovered motor activity, expressed by the EMGRMS, in all groups, in pre vs. post (30 or 60 days) comparisons.This is the peer-reviewed version of the article: Krueger, E., Magri, L.M.S., Botelho, A.S., Bach, F.S., Rebellato, C.L.K., Fracaro, L., Fragoso, F.Y.I., Villanova, J.A., Brofman, P.R.S., Popović-Maneski, L., 2019. Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI. Neuroscience Letters 696, 38–45. [https://doi.org/10.1016/j.neulet.2018.12.004]Published version: [https://hdl.handle.net/21.15107/rcub_dais_4595
The importance of the Thr17 residue of phospholamban as a phosphorylation site under physiological and pathological conditions
Brazilian minipig as a large-animal model for basic research and stem cell-based tissue engineering. Characterization and in vitro differentiation of bone marrow-derived mesenchymal stem cells
EVIDENCE THAT BODY IRRITATIONS OR EMOTIONS RETARD GASTRIC EVACUATION, NOT BY PRODUCING PYLOROSPASM BUT BY DEPRESSING GASTRIC MOTILITY 1
Einfluß der Muskelarbeit und der einseitigen Okklusion der Arteria pulmonalis auf die Belastung des kardiopulmonalen Systems
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