The Effectiveness of Biopreparations on the Qualitative Properties of Feedstuff

It has been shown that in the process of enrichment of different green grass (silage), using a consortium endemic probiotic LAB strains (which sintesized bacteriocins) and yeast strain of Kluveromyces marxsianus 83(which synthesizing mycocin) led to increasing amounts of essential amino acids (in particular lysine and methionine) and protein. It has been shown that in the content of spore microflora, mold, bacteria contaminating silage was decreased with respect to the control. The technological parameters of silage have been determined

The Effectiveness of Biopreparations on the Qualitative Properties of Feedstuff

It has been shown that in the process of enrichment of different green grass (silage), using a consortium endemic probiotic LAB strains (which sintesized bacteriocins) and yeast strain of Kluveromyces marxsianus 83(which synthesizing mycocin) led to increasing amounts of essential amino acids (in particular lysine and methionine) and protein. It has been shown that in the content of spore microflora, mold, bacteria contaminating silage was decreased with respect to the control. The technological parameters of silage have been determined

THE URGENCY OF THE PROBLEM OF RESIDUAL NEUROMUSCULAR BLOCK AFTER SINGLE INTRAOPERATIVE RELAXANT ADMINISTRATION IN NEUROSURGICAL PATIENTS: PRELIMINARY DATA

Under the present-day conditions, it is necessary to allow for rapid postoperative awakening in each neurosurgical patient, which is impossible under residual neuromuscular block. The paper gives the preliminary data of a prospective observational study of the frequency of residual neuromuscular block after single intraoperative administration of a moderate- or long-acting myorelaxant. When the myorelaxants are used once according to the standard procedure (in the absence of TOF monitoring and pharmacological elimination of the action of myorelaxants), the rate of residual myorelaxation in neurosurgical patients has been found to be unacceptably high and to hinder their rapid awakening and neurological assessment. The administration of pipecuronium gives rise to residual block in all cases. That of rocuronium makes it possible to reduce the rate of residual myorelaxation, but not to eliminate it completely. Residual block can be preserved 2–4.5 hours after single administration of rocuronium or pipecuronium

POSTTREATMENT NEUROBLASTOMA MATURATION TO GANGLIONIC CELL TUMOR

Tumor cells can differentiate into more mature forms in undifferentiated or poorly differentiated tumors, such as medulloblastomas with increased nodularity, as well as neuroblastomas. The authors describe 2 cases of neuroblastoma maturation into ganglioneuroblastoma 5 months after chemotherapy in a 2-year-old girl and 3 years after radiotherapy in a 16-year-old girl

Integrated stratigraphy of the Bartonian-Priabonian Urtsadzor section, Armenia

The uppermost Bartonian to lowermost Priabonian transition is associated with several rapid extinctions across microfossil groups. However, the marker for the Bartonian-Priabonian boundary is not formally defined and currently under discussion. The planktonic foraminifera Acarinina and Morozovelloides suffer a rapid global extinction and calcareous nannofossils show several assemblage changes including the last occurrences of Sphenolithus obtusus and Chiasmolithus grandis, the acme of Cribrocentrum erbae and first occurrences of Chiasmolithus oamaruensis, Isthmolithus recurvus and Cribrocentrum isabellae around this interval. Within the shallow water, larger foraminifera also show an extinction of the large species of the genus Nummulites, as well as the first occurrences of important genera, such as Spiroclypeus and Pellatispira the upper middle Eocene. However, the exact correlation between shallow and deep water records remains uncertain, as do the mechanisms driving these extinction events. Here we present the first results of a new integrated stratigraphical study (calcareous nannofossils, planktonic foraminifera, larger benthic foraminifera, and magnetostratigraphy) of the Urtsadzor section in south-western Armenia which appears to be continuous through this interval. The Urtsadzor section consists of marlstones rich in micro- and nannofossils, with interbedded limestones containing abundant larger foraminifera. Our new data enable us to correlate larger foraminiferal events with global planktonic biostratigraphy, in a section outside of southwest Europe where most previous correlations have been based. At Urtsadzor, the large Nummulites species N. millecaput, appear to decrease in abundance toward the top of the section. The first occurrence of Spiroclypeus occurs in the uppermost limestone bed. These larger foraminiferal events occur well above the planktonic foraminiferal extinction level and nannofossil assemblage changes indicating the event is not synchronous across all groups, and has important implications for biostratigraphy

Doxorubicin-enriched, ALDH^br mouse breast cancer stem cells are treatable to oncolytic herpes simplex virus type 1

<p>Abstract</p> <p>Background</p> <p>The primary objective of this study was to test whether oncolytic herpes simplex virus type 1 (HSV1) could eradicate chemoresistant cancer stem cells (CSCs).</p> <p>Methods</p> <p>The fluorescent aldefluor reagent-based technique was used to identify and isolate ALDH^br cells as CSCs from the 4T1 murine breast cancer cell line. The presence of ALDH^br 4T1 cells was also examined in 4T1 breast cancer transplanted in immune-competent syngeneic mice.</p> <p>Results</p> <p>Compared with ALDH^lo cells, ALDH^br cells had a markedly higher ability to form tumor spheres <it>in vitro</it> and a higher tumorigenic potential <it>in vivo</it>. ALDH^br cells also exhibited increased doxorubicin resistance <it>in vitro</it>, which correlated with a selective increase in the percentage of ALDH^br cells after doxorubicin treatment and an increased expression of P-glycoprotein (P-gp), a known chemoresistance factor. In contrast, oncolytic HSV1 was able to kill ALDH^br cells <it>in vitro</it> and even more markedly <it>in vivo</it>. Furthermore, in <it>in vivo</it> studies, systemic administration of doxorubicin followed by intratumoral injection of oncolytic HSV1 resulted in much more significant suppression of tumor growth with increased median survival period compared with each treatment given alone (p<0.05). Though more CD8⁺ T lymphocytes were induced by oncolytic HSV1, no significant specific T cell response against CSCs was detected <it>in vivo</it>.</p> <p>Conclusions</p> <p>These results suggested that the use of oncolytic HSV1 following doxorubicin treatment may help eradicate residual chemoresistant CSCs <it>in vivo</it>.</p