Integrated approach to radiodiagnosis of follicular thyroid neoplasia: a retrospective cohort trial

Background. An evidence-based diagnostic tactics for follicular thyroid gland neoplasia is lacking to date. First-line priority are radiography diagnostic techniques, which vary in capacities and therefore must be regulated in use.Objectives. An efficacy evaluation of multiparametric ultrasound (US), sonoelastography (SEG) and radionuclide scintigraphy (RS) in diagnosis of follicular thyroid neoplasms (FTN).Methods. Preoperative examination was interpreted in 222 FTN patients (86 with follicular thyroid adenoma, FTA, and 136 with follicular thyroid cancer, FTC) with subsequent surgery. A retrospective statistical data analysis was performed for B-mode US, colour Doppler imaging (CDI), power Doppler imaging (PDI), sonoelastography and Tc-99m pertechnetate scintigraphy.Results. Novel FTN descriptive evidence has been obtained. Particularly, an FTA vs. FTC trait comparison showed no reliable US marker of a node assignment to FTA or FTC. Trials of the national-manufactured TI-RADS system showed its good diagnostic potential: FTN sensitivity 89.55, specificity 77.58 and accuracy 83.52%. A SEG picture of FTN was typically motley-colour and mosaic. Young’s modulus in FTA was 27.5 ± 7.1 kPa, a higher stiffness (62.1 ± 12.1 kPa) in FTC indicated a higher likelihood of malignancy. Scintigraphy exhibited a modest capacity for FTN diagnosis (sensitivity 86.67, specificity 48.08 and accuracy 56.72%). AUC values (0.617) indicate its limited use for differential FTN diagnosis, mainly in hyperfunctioning nodules. Our experience elaborated an original algorithm for radiographic techniques application in FTN diagnosis.Conclusion. Several radiographic methods are warranted in suspected FTN. First-line is multiparametric US B-mode imaging to detect FTN priority markers and US symptom complexes. Sonoelastography is second-line in ambiguous cases to further clarify structure (stiffness) of the thyroid nodule examined. Unlike SEG, scintigraphy assesses the functional traits of thyroid nodule and so has limited indications, an important factor to consider in FTN

Study Of Technical Cellulose As A Matrix-Sorbent To Develop Express Analytic System For Water Safety Control

The study presented by the authors is devoted to the study of the properties and the possibility of using technical cellulose from non-wood plant raw materials as a solid-phase matrix to obtain solid-phase reactive indicator systems by the following methods: synthesis method on the base of a hetarylformazane immobilized on a cellulose matrix and development of analytical systems based on preconcentration of the determined metal ion by a matrix with subsequent its «revealing» by the formazan («revealing» method). The article focuses on determination of optimal combinations of chromogenic organic reagents (hetarylformazanes) and cellulose-based matrices for developing solid-phase reaction-based indicator systems. Adsorption features of formazan reagents onto cellulose matrices was studied. It has been established the relation between the reagent molecule structure, composition of cellulose matrix and analytical properties of the test-systems synthesized to determine metal ions. Different approaches were developed and applied to reveal the visually observable and easily measured effect due to cellulose properties as well as properties of hetarylformazanes fixed on the surface of the matrix. This fact allows to control sensitivity and selectivity of solid-phase reactive indicator systems for water quality assessment. © 2021 Altai State University. All rights reserved

Improvement of Technology of Cholera Toxin B-Subunit Production

Consideration is given to implementation of state-of-the-art filtration technologies for up-scaled manufacturing of cholera toxin B-subunit, produced by recombinant Vibrio cholerae non O1 KM93 strain. Selected are micro- and ultra-filtration membranes to be incorporated into manufacturing method. Investigated are the properties of cholera toxin B-subunit, obtained applying the pilot technology. The engineered method for up-scaled manufacturing of cholera toxin B-subunit makes the procedure easier-to-maintain due to tangential micro- and ultra-filtration, performed at the stage of purification and concentration. It excludes labor-consuming chromatographic purification, while retaining B-subunit properties. The studies undertaken make it possible to manufacture cholera toxin B-subunit with the same characteristics as in the case of the pilot technology, but under production conditions, and use it as a component for chemical cholera vaccine

EVALUATION OF VIBRIO CHOLERAE 569B INABA PROTECTIVE ANTIGENES, DERIVED ON INDUSTRIAL AND DEVELOPED BIOREACTORS AS WELL AS BY IMPROVED TECHNOLOGY

We evaluated immunochemical, physical and biochemical properties of Vibrio cholerae 569B INABA protective antigenes, derived on industrial and own-developed bioreactors as well as by technology of its concentration by tangential ultrafiltration. We detected, protease, twinase and. lysophospholipase in all samples. Also, dotimmunoanalysis showed equal concentration, of cholerogen-anatoxine and. O-antigen in all samples too. Using chromatography and. electrophoresis, we found their properties as similar. Thus, we suppose to be possible using developed bioreactor as well as technology of Vibrio cholerae 569B INABA protective antigens concentration by tangential concentration during a process of synthetic oral cholera vaccine production

Properties of Experimental and Standard Preparations of the Protective O Antigen of <I>Vibrio cholerae</I> M41 Ogawa

Investigated are immunochemical, chemical and biochemical properties of the O-antigen preparations obtained from Vibrio cholerae M41 classical biovar, serotype Ogawa strain using standard manufacturing and improved technologies of their concentrating. The preparations have been concentrated by ultrafiltration in the mode of tangential liquid flow with membranes of various cut-off levels for a particular molar weight. ELISA has revealed equal O-antigen load in all of these preparations. Studies of specific fractures by means of chromatographic and electrophoretic methods have demonstrated their properties to be alike. Chemical composition analysis has also identified coincidence in the load of components under specification. Thus, it has been proved that application of the improved V. cholerae protective antigen concentrating technology for manufacturing cholera chemical bivalent palletized vaccine is justified

Methods and Technologies of Cholera Vibrio Cultivation (Scientific Review)

Displayed is the review of domestic and foreign literature sources devoted to matters of cholera vibrio cultivation. Discussed is the information concerning the following methods utilized in the technological process of vibrio growth stimulation: batch cultivation, fed-batch, fermentation and dialysis, periodic and continuous cultivation. Analyzed is the impact of such parameters as dissolved oxygen concentration, count of carbon nutrition source, medium pH, temperature rate , concentration and physiological condition of inoculate, duration of technological process, affecting the growth of this microorganism and synthesis of its antigens. Consequently, literature data analysis has contributed to the selection of proper method for cholera vibrio cultivation and consideration of the factors mentioned above for the development of manufacture technology applied to the production of medical immunoglobulin preparations for diagnostics and prophylaxis of cholera

Клеточные маркеры непрогредиентности при респираторном оксалозе

Respiratory oxalosis (RO), a special hereditary form of obstructive lung disease accompanied by hyperoxaluria, non-progredient course, absence of allergy and several cytology markers was studied in this comparative prospective study. We have observed 2 groups of non-smoking women (71 patients with RO and 64 patients with asthma accompanied by allergy with progredient course during 5 years). We evaluated the locomotor function of the mononuclear and polynuclear blood phagocytes using the inhibition of lymphocyte migration test as an immunological marker, and the hepatocyte function using AST / ALT ratio as a cytological marker. Their prevalence was the greatest in the 1-st group and constituted 100 % for the immunological marker and 98 % for the cytological one. We assume that function of the mononuclear phagocytes in RO probably results in the non progredient course of the disease. It relates to congenital high threshold of the immunocompetent cell sensitivity to polyclonal mitogens.Респираторный оксалоз (РО) является особой наследственной формой обструктивной болезни легких, сопровождаемой гипероксаурией, непрогредиентным течением, отсутствием аллергических проявлений и наличием ряда цитологических маркеров. Мы проводили сравнительное проспективное исследование в течение 5 лет в 2 группах пациентов (все некурящие женщины): 71 человек с РО и 64 с атопической бронхиальной астмой, аллергическими проявлениями и прогредиентным течением. Оценивали локомоторную функцию моно- и полинуклеарных фагоцитов периферической крови в тесте РТМЛ после инкубации крови с поликлональным митогеном FGA (цитологический маркер) и функцию гепатоцитов в тесте де Ритиса с учетом соотношения трансаминаз АСТ и АЛТ (иммунологический маркер). Встречаемость этих маркеров была максимальной в 1-й группе больных и составила 100 % для иммунологического маркера и 98 % — для цитологического маркера. Таким образом, мы полагаем, что ведущим фактором формирования непрогредиентного течения РО является наследственно обусловленный высокий порог клеточной чувствительности иммунокомпетентных клеток к поликлональному митогену