Performance of LED-Based Fluorescence Microscopy to Diagnose Tuberculosis in a Peripheral Health Centre in Nairobi.

Sputum microscopy is the only tuberculosis (TB) diagnostic available at peripheral levels of care in resource limited countries. Its sensitivity is low, particularly in high HIV prevalence settings. Fluorescence microscopy (FM) can improve performance of microscopy and with the new light emitting diode (LED) technologies could be appropriate for peripheral settings. The study aimed to compare the performance of LED-FM versus Ziehl-Neelsen (ZN) microscopy and to assess feasibility of LED-FM at a low level of care in a high HIV prevalence country

Reducing the Number of Sputum Samples Examined and Thresholds for Positivity: An Opportunity to Optimise Smear Microscopy.

SETTING: Urban health clinic, Nairobi. OBJECTIVE: To evaluate the impact on tuberculosis (TB) case detection and laboratory workload of reducing the number of sputum smears examined and thresholds for diagnosing positive smears and positive cases. DESIGN: In this prospective study, three Ziehl-Neelsen stained sputum smears from consecutive pulmonary TB suspects were examined blind. The standard approach (A), > or = 2 positive smears out of 3, using a cut-off of 10 acid-fast bacilli (AFB)/100 high-power fields (HPF), was compared with approaches B, > or = 2 positive smears (> or = 4 AFB/100 HPF) out of 3, one of which is > or = 10 AFB/100 HPF; C, > or = 2 positive smears (> or = 4 AFB/100 HPF) out of 3; D, > or = 1 positive smear (> or = 10 AFB/100 HPF) out of 2; and E, > or = 1 positive smear (> or = 4 AFB/100 HPF) out of 2. The microscopy gold standard was detection of at least one positive smear (> or = 4 AFB/100 HPF) out of 3. RESULTS: Among 644 TB suspects, the alternative approaches detected from 114 (17.7%) (approach B) to 123 cases (19.1%) (approach E) compared to 105 cases (16.3%) for approach A (P < 0.005). Sensitivity ranged between 82.0% (105/128) for A and 96.1% (123/128) for E. The single positive smear approaches reduced the number of smears by 36% compared to approach A. CONCLUSION: Reducing the number of specimens and the positivity threshold to define a positive case increased the sensitivity of microscopy and reduced laboratory workload

From angle-action to Cartesian coordinates: A key transformation for molecular dynamics

The transformation from angle-action variables to Cartesian coordinates is a crucial step of the (semi) classical description of bimolecular collisions and photo-fragmentations. The basic reason is that dynamical conditions corresponding to experiments are ideally generated in angle-action variables whereas the classical equations of motion are ideally solved in Cartesian coordinates by standard numerical approaches. To our knowledge, the previous transformation is available in the literature only for triatomic systems. The goal of the present work is to derive it for polyatomic ones.Comment: 10 pages, 11 figures, submitted to J. Chem. Phy

Estimations for the Single Diffractive production of the Higgs boson at the Tevatron and the LHC

The single diffractive production of the standard model Higgs boson is computed using the diffractive factorization formalism, taking into account a parametrization for the Pomeron structure function provided by the H1 Collaboration. We compute the cross sections at next-to-leading order accuracy for the gluon fusion process, which includes QCD and electroweak corrections. The gap survival probability ($$) is also introduced to account for the rescattering corrections due to spectator particles present in the interaction, and to this end we compare two different models for the survival factor. The diffractive ratios are predicted for proton-proton collisions at the Tevatron and the LHC for the Higgs boson mass of $M_H$ = 120 GeV. Therefore, our results provide updated estimations for the diffractive ratios of the single diffractive production of the Higgs boson in the Tevatron and LHC kinematical regimes.Comment: 20 pages, 6 figures, 3 table

Un procedimiento para obtener clusters utilizando la D.V.S. de una matriz. Comparaciones con el biplot y con el modelo Q-factorial

Durante las últimas décadas, el análisis de un conjunto de n individuos medidos en p variables, proporcionando una matriz de datos Xn,p, mediante técnicas de representación que utilizan la Descomposición en Valores Singulares de la matriz Xn,p (o alguna derivada), han permitido resumir la información que aportan los datos en alguna forma óptima, siendo muy útil para indicar la presencia de clusters entre los n individuos y/o para prevenir ante posibles clasificaciones erróneas producidas por técnicas de agrupamiento más complejas. En este artículo estudiaremos un procedimiento que puede utilizarse en ocasiones para obtener clasificaciones naturales de un conjunto de datos, basado en la representación biplot y en el modelo Q-factorial que puede obtenerse a partir de la DVS

Death rates from malaria epidemics, Burundi and Ethiopia.

Death rates exceeded emergency thresholds at 4 sites during epidemics of Plasmodium falciparum malaria in Burundi (2000-2001) and in Ethiopia (2003-2004). Deaths likely from malaria ranged from 1,000 to 8,900, depending on site, and accounted for 52% to 78% of total deaths. Earlier detection of malaria and better case management are needed

Tuberculosis treatment in a refugee and migrant population: 20 years of experience on the Thai-Burmese border.

Although tuberculosis (TB) is a curable disease, it remains a major global health problem and an important cause of morbidity and mortality among vulnerable populations, including refugees and migrants

The method of Gaussian weighted trajectories. V. On the 1GB procedure for polyatomic processes

In recent years, many chemical reactions have been studied by means of the quasi-classical trajectory (QCT) method within the Gaussian binning (GB) procedure. The latter consists in "quantizing" the final vibrational actions in Bohr spirit by putting strong emphasis on the trajectories reaching the products with vibrational actions close to integer values. A major drawback of this procedure is that if N is the number of product vibrational modes, the amount of trajectories necessary to converge the calculations is ~ 10^N larger than with the standard QCT method. Applying it to polyatomic processes is thus problematic. In a recent paper, however, Czako and Bowman propose to quantize the total vibrational energy instead of the vibrational actions [G. Czako and J. M. Bowman, J. Chem. Phys., 131, 244302 (2009)], a procedure called 1GB here. The calculations are then only ~ 10 times more time-consuming than with the standard QCT method, allowing thereby for considerable numerical saving. In this paper, we propose some theoretical arguments supporting the 1GB procedure and check its validity on model test cases as well as the prototype four-atom reaction OH+D_2 -> HOD+D

Sequential fissions of heavy nuclear systems

In Xe+Sn central collisions from 12 to 20 MeV/A measured with the INDRA 4$\pi$ multidetector, the three-fragment exit channel occurs with a significant cross section. In this contribution, we show that these fragments arise from two successive binary splittings of a heavy composite system. Strong Coulomb proximity effects are observed in the three-fragment final state. By comparison with Coulomb trajectory calculations, we show that the time scale between the consecutive break-ups decreases with increasing bombarding energy, becoming compatible with quasi-simultaneous multifragmentation above 18 MeV/A.Comment: 6 pages, 5 figures, contribution to conference proceedings of the Fifth International Workshop on Nuclear fission and Fission-Product Spectroscop

Recomendação de espécies arbóreas nativas, por tipo de solo, para recuperação ambiental das margens da Represa do Rio Iraí, Pinhais, PR.

A construção de reservatórios de água próximos aos grandes centros urbanos é uma realidade crescente nos dias atuais. Esse tipo de empreendimento, em geral, causa forte supressão de florestas fluviais, gerando, portanto, a necessidade de plantios logo acima da cota de máxima inundação que venham a compensar a mencionada supressão. Este trabalho tem por objetivo recomendar espécies arbóreas nativas para plantios, considerando grau de adaptabilidade dessas ao nível de hidromorfia dos solos presentes nas paisagens circunjacentes ao reservatório do rio Iraí, localizado no município de Pinhais, Paraná.Nota técnica