34,555 research outputs found
Testing for Photometric Variability at the L/T Boundary
We construct K_s-band light curves for nine field L and T
brown dwarfs using the Palomar 60 inch Telescope. Results of a robust
statistical analysis indicate that about half the targets show significant
evidence for variability. Two of these variable targets have marginally
significant peaks in the Lomb-Scargle periodogram. The phased light
curves show evidence for periodic behavior on timescales of about 1.5
and 3.0 hours
Structural modeling and functional analysis of the essential ribosomal processing protease Prp from Staphylococcus aureus
In Firmicutes and related bacteria, ribosomal large subunit protein L27 is encoded with a conserved N-terminal extension that is removed to expose residues critical for ribosome function. Bacteria encoding L27 with this N-terminal extension also encode a sequence-specific cysteine protease, Prp, which carries out this cleavage. In this work, we demonstrate that L27 variants with an un-cleavable N-terminal extension, or lacking the extension (pre-cleaved), are unable to complement an L27 deletion in Staphylococcus aureus. This indicates that N-terminal processing of L27 is not only essential but possibly has a regulatory role. Prp represents a new clade of previously uncharacterized cysteine proteases, and the dependence of S. aureus on L27 cleavage by Prp validates the enzyme as a target for potential antibiotic development. To better understand the mechanism of Prp activity, we analyzed Prp enzyme kinetics and substrate preference using a fluorogenic peptide cleavage assay. Molecular modeling and site-directed mutagenesis implicate several residues around the active site in catalysis and substrate binding, and support a structural model in which rearrangement of a flexible loop upon binding of the correct peptide substrate is required for the active site to assume the proper conformation. These findings lay the foundation for the development of antimicrobials that target this novel, essential pathway
Integrable theories and loop spaces: fundamentals, applications and new developments
We review our proposal to generalize the standard two-dimensional flatness
construction of Lax-Zakharov-Shabat to relativistic field theories in d+1
dimensions. The fundamentals from the theory of connections on loop spaces are
presented and clarified. These ideas are exposed using mathematical tools
familiar to physicists. We exhibit recent and new results that relate the
locality of the loop space curvature to the diffeomorphism invariance of the
loop space holonomy. These result are used to show that the holonomy is abelian
if the holonomy is diffeomorphism invariant.
These results justify in part and set the limitations of the local
implementations of the approach which has been worked out in the last decade.
We highlight very interesting applications like the construction and the
solution of an integrable four dimensional field theory with Hopf solitons, and
new integrability conditions which generalize BPS equations to systems such as
Skyrme theories. Applications of these ideas leading to new constructions are
implemented in theories that admit volume preserving diffeomorphisms of the
target space as symmetries. Applications to physically relevant systems like
Yang Mills theories are summarized. We also discuss other possibilities that
have not yet been explored.Comment: 64 pages, 8 figure
Molecular dynamics simulations of ballistic annihilation
Using event-driven molecular dynamics we study one- and two-dimensional
ballistic annihilation. We estimate exponents and that describe
the long-time decay of the number of particles () and of
their typical velocity (). To a good accuracy our results
confirm the scaling relation . In the two-dimensional case our
results are in a good agreement with those obtained from the Boltzmann kinetic
theory.Comment: 4 pages; some changes; Physical Review E (in press
Current induced transverse spin-wave instability in thin ferromagnets: beyond linear stability analysis
A sufficiently large unpolarized current can cause a spin-wave instability in
thin nanomagnets with asymmetric contacts. The dynamics beyond the instability
is understood in the perturbative regime of small spin-wave amplitudes, as well
as by numerically solving a discretized model. In the absence of an applied
magnetic field, our numerical simulations reveal a hierarchy of instabilities,
leading to chaotic magnetization dynamics for the largest current densities we
consider.Comment: 14 pages, 10 figures; revtex
Hotspots of corruption: Applying a problem-oriented approach to preventing corruption in the public sector
âThe final publication is available at Springer via http://dx.doi.org/10.1057/sj.2013.38.".
© Palgrave Macmillan, a division of Macmillan Publishers Ltd 2013Some places have no crime and some have a lot, and thus we study hotspots. Corruption is structured differently to crime, but hotspots still are notable. The difference is that hotspots are not places but clusters of activity. This article analyses corruption cases from New York City to explore a way of identifying such clusters. Seventy-two cases were coded according to features that represent the elements of the crime triangle: offender and motivation, target and opportunity, and place and ability. Multidimensional scaling revealed three groups of cases, exhibiting different patterns of corrupt activity. Group one involved politicians involved in high value financial corruption. Group two primarily involved supervisors who created opportunities involving procurement and contracts. Group three involved inspectors, particularly in the infrastructure sector, who were involved with low value bribes to violate regulations. Each is discussed in relation to situational crime prevention principles to develop possible strategies for prevention
Genome-Wide Transposon Screen of a Pseudomonas syringae mexB Mutant Reveals the Substrates of Efflux Transporters.
Bacteria express numerous efflux transporters that confer resistance to diverse toxicants present in their environment. Due to a high level of functional redundancy of these transporters, it is difficult to identify those that are of most importance in conferring resistance to specific compounds. The resistance-nodulation-division (RND) protein family is one such example of redundant transporters that are widespread among Gram-negative bacteria. Within this family, the MexAB-OprM protein complex is highly expressed and conserved among Pseudomonas species. We exposed barcoded transposon mutant libraries in isogenic wild-type and ÎmexB backgrounds in P. syringae B728a to diverse toxic compounds in vitro to identify mutants with increased susceptibility to these compounds. Mutants with mutations in genes encoding both known and novel redundant transporters but with partially overlapping substrate specificities were observed in a ÎmexB background. Psyr_0228, an uncharacterized member of the major facilitator superfamily of transporters, preferentially contributes to tolerance of acridine orange and acriflavine. Another transporter located in the inner membrane, Psyr_0541, contributes to tolerance of acriflavine and berberine. The presence of multiple redundant, genomically encoded efflux transporters appears to enable bacterial strains to tolerate a diversity of environmental toxins. This genome-wide screen performed in a hypersusceptible mutant strain revealed numerous transporters that would otherwise be dispensable under these conditions. Bacterial strains such as P. syringae that likely encounter diverse toxins in their environment, such as in association with many different plant species, probably benefit from possessing multiple redundant transporters that enable versatility with respect to toleration of novel toxicants.IMPORTANCE Bacteria use protein pumps to remove toxic compounds from the cell interior, enabling survival in diverse environments. These protein pumps can be highly redundant, making their targeted examination difficult. In this study, we exposed mutant populations of Pseudomonas syringae to diverse toxicants to identify pumps that contributed to survival in those conditions. In parallel, we examined pump redundancy by testing mutants of a population lacking the primary efflux transporter responsible for toxin tolerance. We identified partial substrate overlap for redundant transporters, as well as several pumps that appeared more substrate specific. For bacteria that are found in diverse environments, having multiple, partially redundant efflux pumps likely allows flexibility in habitat colonization
An experimental investigation of the relationships among race, prayer, and pain
Background and aims
Compared to White individuals, Black individuals demonstrate a lower pain tolerance. Research suggests that differences in pain coping strategies, such as prayer, may mediate this race difference. However, previous research has been cross-sectional and has not determined whether prayer in and of itself or rather the passive nature of prayer is driving the effects on pain tolerance. The aim of this study was to clarify the relationships among race, prayer (both active and passive), and pain tolerance.
Methods
We randomly assigned 208 pain-free participants (47% Black, 53% White) to one of three groups: active prayer (âGod, help me endure the painâ), passive prayer (âGod, take the pain awayâ), or no prayer (âThe sky is blueâ). Participants first completed a series of questionnaires including the Duke University Religion Index, the Coping Strategies Questionnaire-Revised (CSQ-R), and the Pain Catastrophizing Scale. Participants were then instructed to repeat a specified prayer or distractor coping statement while undergoing a cold pressor task. Cold pain tolerance was measured by the number of seconds that had elapsed while the participantâs hand remained in the cold water bath (maximum 180 s).
Results
Results of independent samples t-tests indicated that Black participants scored higher on the CSQ-R prayer/hoping subscale. However, there were no race differences among other coping strategies, religiosity, or catastrophizing. Results of a 2 (Race: White vs. Black)Ă3 (Prayer: active vs. passive vs. no prayer) ANCOVA controlling for a general tendency to pray and catastrophizing in response to prayer indicated a main effect of prayer that approached significance (p=0.06). Pairwise comparisons indicated that those in the active prayer condition demonstrated greater pain tolerance than those in the passive (p=0.06) and no prayer (p=0.03) conditions. Those in the passive and no prayer distractor conditions did not significantly differ (p=0.70). There was also a trending main effect of race [p=0.08], with White participants demonstrating greater pain tolerance than Black participants.
Conclusions
Taken together, these results indicate that Black participants demonstrated a lower pain tolerance than White participants, and those in the active prayer condition demonstrated greater tolerance than those in the passive and no prayer conditions. Furthermore, Black participants in the passive prayer group demonstrated the lowest pain tolerance, while White participants in the active prayer group exhibited the greatest tolerance. Results of this study suggest that passive prayer, like other passive coping strategies, may be related to lower pain tolerance and thus poorer pain outcomes, perhaps especially for Black individuals. On the other hand, results suggest active prayer is associated with greater pain tolerance, especially for White individuals.
Implications
These results suggest that understanding the influence of prayer on pain may require differentiation between active versus passive prayer strategies. Like other active coping strategies for pain, active prayer may facilitate self-management of pain and thus enhance pain outcomes independent of race. Psychosocial interventions may help religiously-oriented individuals, regardless of race, cultivate a more active style of prayer to improve their quality of life
Dose-dependent effects of a soluble dietary fibre (pectin) on food intake, adiposity, gut hypertrophy and gut satiety hormone secretion in rats
Acknowledgments We thank Donna Wallace and Animal House staff at the Rowett Institute of Nutrition and Health for the daily care of experimental rats and for the body weight, food intake and MRI measurements.Peer reviewedPublisher PD
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