Contribution of viscous shear to friction in cold rolling of low-carbon steel

This work shows that for temperatures, pressures and shear rates that are common in cold rolling of low-carbon steel, viscous shear stress significantly contributes to the total friction force. Experiments were carried out to validate the theory on lubricant film formation and elasto-hydrodynamic lubrication, both with laboratory scale tribometers and a semi-industrial pilot mill facility. These experiments showed that at high shear rates, that are common in cold rolling, the lubricant does not behave as a Newtonian fluid anymore; moreover the viscosity at high pressure cannot accurately be described by a simple exponential law. With the correct relations implemented in a rolling model, both rolling force and forward slip are predicted with good accuracy for hydrodynamically lubricated cold rolling experiments.</p

Insights into dynamin-associated disorders through analysis of equivalent mutations in the yeast dynamin Vps1

The dynamins represent a superfamily of proteins that have been shown to function in a wide range of membrane fusion and fission events. An increasing number of mutations in the human classical dynamins, Dyn-1 and Dyn-2 has been reported, with diseases caused by these changes ranging from Charcot-Marie-Tooth disorder to epileptic encephalopathies. The budding yeast, Saccharomyces cerevisiae expresses a single dynamin-related protein that functions in membrane trafficking, and is considered to play a similar role to Dyn-1 and Dyn-2 during scission of endocytic vesicles at the plasma membrane. Large parts of the dynamin protein are highly conserved across species and this has enabled us in this study to select a number of disease causing mutations and to generate equivalent mutations in Vps1. We have then studied these mutants using both cellular and biochemical assays to ascertain functions of the protein that have been affected by the changes. Specifically, we demonstrate that the Vps1-G397R mutation (Dyn-2 G358R) disrupts protein oligomerization, Vps1-A447T (Dyn-1 A408T) affects the scission stage of endocytosis, while Vps1-R298L (Dyn-1 R256L) affects lipid binding specificity and possibly an early stage in endocytosis. Overall, we consider that the yeast model will potentially provide an avenue for rapid analysis of new dynamin mutations in order to understand the underlying mechanisms that they disrupt

Quantification of the In fluence of anisotropic plastic yielding on cold rolling force

Anisotropic mechanical properties in sheet material are of high importance, in particular for material to be used in stamping or deep drawing applications. The cold rolling process has a marked influence on the degree of anisotropy in the final product. However, the influence of material anisotropy on the cold rolling process itself has so far not been rigorously investigated. In this work, the degree of normal anisotropy is determined for two low-carbon steel grades after various degree of cold rolling reduction, both by X-ray diffraction as well as by tensile testing. The experimental work shows that material with high cold rolling reduction has non-negligible anisotropic properties; as a consequence the rolling force is seriously overestimated by cold rolling models with an isotropic yield criterion. It is therefore proposed to use the Hill48 yield criterion (instead of the von Mises criterion) in cold rolling models, this criterion takes anisotropic material behaviour into account. A comparison of cold rolling experiments with model predictions confirms that including the Hill48 yield criterion significantly improves the accuracy of a cold rolling model.</p

Associations between autonomic nervous system activity and risk-taking and internalizing behavior in young adolescents

Dysregulated autonomic nervous system (ANS) activity has been associated with adolescent risk-taking and internalizing behavior, but previous results in community samples have been mixed. We investigated whether ANS activity was associated with higher risk-taking and internalizing behavior in young adolescents (age 11/12; n = 875), and whether adolescents' gender, parents' parenting style or a combination of both moderated these associations. Adolescents and their parents were recruited as part of the population-based, longitudinal Amsterdam Born Children and their Development (ABCD) study. Risk-taking behavior was assessed with the Balloon Analogue Risk Task and the personality characteristics sensation seeking and impulsivity, measured with the Substance Use Risk Profile Scale (SURPS). Internalizing behavior was assessed via the SURPS subscales anxiety sensitivity and hopelessness. Authoritative (AUTH-SW) and authoritarian (AUTH-S) parenting styles were measured with the Parenting Styles and Dimensions Questionnaire. Resting ANS activity was assessed via heart rate and respiratory sinus arrhythmia (RSA). Hierarchical, multivariable regression analyses showed higher RSA, but not heart rate, being associated with higher risk-taking behavior and sensation seeking. The associations between ANS activity and risk-taking variables were not significantly moderated by gender, parenting, or interactions between gender and parenting. Our findings suggest that RSA activity may be a relevant factor in mild to moderate risk-taking behavior in adolescents from the general population, regardless of their gender or the type of parenting they experience

αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish

Cadherin complexes mediate cell-cell adhesion and are crucial for embryonic development. Besides their structural function, cadherin complexes also transduce tension across the junction-actomyosin axis into proportional biochemical responses. Central to this mechanotransduction is the stretching of the cadherin-F-actin-linker α-catenin, which opens its central domain for binding to effectors such as vinculin. Mechanical unfolding of α -catenin leads to force-dependent reinforcement of cadherin-based junctions as studied in cell culture. The importance of cadherin mechanotransduction for embryonic development has not been studied yet. Here we used TALEN-mediated gene disruption to perturb endogenous αE-catenin in zebrafish development. Zygotic α-catenin mutants fail to maintain their epithelial barrier, resulting in tissue rupturing. We then specifically disrupted mechanotransduction, while maintaining cadherin adhesion, by expressing an αE-catenin construct in which the mechanosensitive domain was perturbed. Expression of either wild-type or mechano-defective α-catenin fully rescues barrier function in α-catenin mutants. Expression of mechano-defective α-catenin, however, also induces convergence and extension defects. Specifically, the polarization of cadherin-dependent, lamellipodia-driven cell migration of the lateral mesoderm was lost. These results indicate that cadherin mechanotransduction is crucial for proper zebrafish morphogenesis and uncover one of the essential processes affected by its perturbation