Binary open clusters in the Milky Way: photometric and spectroscopic analysis of NGC 5617 and Trumpler 22

Using photometry and high resolution spectroscopy we investigate for the first time the physical connection between the open clusters NGC 5617 and Trumpler 22. Based on new CCD photometry we report their spatial proximity and common age of ~70 Myr. Based on high resolution spectra collected using the HERMES and UCLES spectrographs on the Anglo-Australian telescope, we present radial velocities and abundances for Fe, Na, Mg, Al, Si, Ca and Ni. The measured radial velocities are -38.63 +/-2.25 km/s for NGC 5617 and -38.46 +/-2.08 km/s for Trumpler 22. The mean metallicity of NGC 5617 was found to be [Fe/H] =-0.18 +/-0.02 and for Trumpler 22 was found to be [Fe/H] = -0.17 +/-0.04. The two clusters share similar abundances across the other elements, indicative of a common chemical enrichment history of these clusters. Together with common motions and ages we confirm that NGC 5617 and Trumpler 22 are a primordial binary cluster pair in the Milky Way.Comment: 7 pages, 3 figure, accepted by MNRA

GISTOMORFOLOGIC FEATURES OF THE REGENERATION OF BONE AND MUCOUS MEMBRANE OF THE MAXILLARY SINUS FLOOR DEFECT WITH DIRECTIONAL TISSUE REGENERATION USING FIRM BRAIN ENVELOPE-BEARING CELLS AND ALLOGRAFTS

Purpose: To evaluate the regenerative capacity of the mucous membrane of the maxillary sinus and osteogenesis in the application of firm brain envelope-bearing cells and allografts (TMO) and bone at perforation during a sinus inlay. Materials and methods: An experimental study conducted at 12 pigs. All completed 24 operational intervention. Over 6 months after the operation and close the defect with the maxillary sinus bottom-bearing cells and allografts from TMO had histological study of samples of bone tissue and mucous membranes.Results: On the surface of the top gnathic sinusitis sinus mucosa, TMO is restored with all its characteristic structural elements. Summary: It is proved that the application of the effective-bearing cells and allografts for bone defect and reconstruction ofmorphological and functional capacity of the top gnathic sinusitis sinus mucosa

Ultrasound diagnostics of left ventricular noncompaction

The clinical cases of rare, genetically heterogeneous cardiomyopathy are described - the left ventricular noncompaction (LVNC) cardiomyopathy for four children (three boys and one girl) in age from two months to 6 years. Description of echocardiography signs is presented in accordance with modern diagnostic criteria. Noncompaction of the left ventricle is recognized in three patients, of the left and right ventricle - in one. In three patients out of four, LVNC was a separate disease, and yet in one it was associated with neurofibromatosis type 1, in combination with dilatation of coronary arteries and pericardial effusion. Three of four patients were admitted with congestive heart failure (CHF) of New York Heart Association (NYHA) functional class 3-4 (FC), and one - with CHF 2 FC. The echocardiograms are brought with description of characteristic features of LVNC (hypertrabecularity, spongy myocardium on the apex of the left ventricle, typical correlation of incompact and compact layer from 2.0 to 2.6). All four patients received treatment for CHF (diuretics, beta-blockers, captopril, in one case - digoxin); two out of four got antithrombotic therapy (aspirin, warfarin). Results of follow-up observation of the patients are described with the estimation of echocardiography signs, including dilatation of coronary arteries. Left ventricular ejection fraction - both by Simpson and Teicholz - increased in two patients, with therapy of chronic heart failure at a follow-up visits over > 5 years simultaneously with the decline of severity of CHF. Another two patients have been examined from 2 months to 5 years and have had CHF 3 FC; their left ventricular ejection fraction increased too, but cardiomegaly and right ventricular heart failure preserved

A rare combination of noncompact myocardium and coronary dilatation with type 1 neurofibromatosis

The article describes the observation of a child with a very rare combination of noncompact left ventricular myocardium and coronary dilatation in type 1 neurofibromatosis (NF1). The first of these complications, undifferentiated cardiomyopathy, also known as noncompact myocardium, is not described in combination with nF 1 in available literature, although cases of hypertrophic cardiomyopathy in patients with NF 1 have been previously reported. Dilatation of the coronary arteries also refers to infrequent manifestations of vascular pathology against the background of neurofibromatosis, and the combination of all three signs (NF 1, noncompact myocardium and coronary dilatation) has not yet been reported by other authors. When examining the patient, we found the characteristic signs of NF 1 (foci of "coffee" pigmentation on the skin, multiple nevuses, fibroids of the forearm, cognitive disorders), electrocardiogram features of left ventricular hypertrophy, negative T-tooth and ST-segment displacement 1 mm below the isoline in Leads V4-6, in echocardiography - pronounced trabecularity, thinning of the compact myocardium layer and a "spongy myocardium" in the region of the left ventricle apex, dilatation of the coronary arteries. With magnetic resonance imaging, signs of noncompact myocardium of the left ventricle were found, with selective coronary angiography - slowing of the coronary blood flow. The patient receives the permanent treatment for heart failure and aspirin, his condition remains stable for 4 years of follow-u

Studying regenerated soybean lines for their useful agronomic and biochemical characteristics

Background. The effect of heavy metal ions can cause ionic stress in plants – the problem of significant interest among researchers. This study was dedicated to the evaluation of genetically modified regenerated soybean lines obtained on nutrient media with heavy metal ions as a mutagenic factor.   Materials and methods. Genetically modified regenerated soybean lines were tested at the Agricultural Biotechnology Laboratory and Soybean Breeding Laboratory, Federal Scientific Center of Agricultural Biotechnology of the Far East named after A. K. Chaika, and at the Agricultural Product Processing Laboratory, All-Russian Research Institute of Soybean.   Object of the study. Fifteen regenerated soybean lines and source cultivars (‘Primorskaya 13’, ‘Primorskaya 301’, ‘Primorskaya 81’, and ‘Hodson’) served as the research material.   Results. Soybean accessions promising for further breeding were selected on the basis of their useful agronomic traits, grain quality, and resistance to fungal diseases. Accessions R 1490 (0.41 kg/m2) and R 1606 (0.38 kg/m2) were identified for having the highest yield in the experiment. Accessions R 1568 (1000 seed weight was 200.0 g) and R 1609 (190.2 g) had the largest seeds. Accessions R 1584, R 1568 and R 1606 manifested complex resistance to fungal diseases under natural conditions, while accession R 1490 was the most resistant under artificial infection pressure. The experiment resulted in selecting a group of lines regenerated from cv. ‘Hodson’ with high content of protein, histidine, valine, methionine, and cysteine. Accessions R 1605 and R 1609 demonstrated the highest oil content. The study revealed changes in the proportions of unsaturated fatty acids С18:2/С18:1 in all regenerant groups

Imbalance in the oxidative stress system-antioxidant protection in patients with chronic pyelonephritis depending on the course of the diseas

Assessment of the imbalance in the system of oxidative stress - antioxidant protection in patients with chronic pyelonephritis depending on the course of the diseas

Skin dendritic cells in melanoma are key for successful checkpoint blockade therapy.

BACKGROUND: Immunotherapy with checkpoint inhibitors has shown impressive results in patients with melanoma, but still many do not benefit from this line of treatment. A lack of tumor-infiltrating T cells is a common reason for therapy failure but also a loss of intratumoral dendritic cells (DCs) has been described. METHODS: We used the transgenic tg(Grm1)EPv melanoma mouse strain that develops spontaneous, slow-growing tumors to perform immunological analysis during tumor progression. With flow cytometry, the frequencies of DCs and T cells at different tumor stages and the expression of the inhibitory molecules programmed cell death protein-1 (PD-1) and T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) on T cells were analyzed. This was complemented with RNA-sequencing (RNA-seq) and real-time quantitative PCR (RT-qPCR) analysis to investigate the immune status of the tumors. To boost DC numbers and function, we administered Fms-related tyrosine 3 ligand (Flt3L) plus an adjuvant mix of polyI:C and anti-CD40. To enhance T cell function, we tested several checkpoint blockade antibodies. Immunological alterations were characterized in tumor and tumor-draining lymph nodes (LNs) by flow cytometry, CyTOF, microarray and RT-qPCR to understand how immune cells can control tumor growth. The specific role of migratory skin DCs was investigated by coculture of sorted DC subsets with melanoma-specific CD8+ T cells. RESULTS: Our study revealed that tumor progression is characterized by upregulation of checkpoint molecules and a gradual loss of the dermal conventional DC (cDC) 2 subset. Monotherapy with checkpoint blockade could not restore antitumor immunity, whereas boosting DC numbers and activation increased tumor immunogenicity. This was reflected by higher numbers of activated cDC1 and cDC2 as well as CD4+ and CD8+ T cells in treated tumors. At the same time, the DC boost approach reinforced migratory dermal DC subsets to prime gp100-specific CD8+ T cells in tumor-draining LNs that expressed PD-1/TIM-3 and produced interferon γ (IFNγ)/tumor necrosis factor α (TNFα). As a consequence, the combination of the DC boost with antibodies against PD-1 and TIM-3 released the brake from T cells, leading to improved function within the tumors and delayed tumor growth. CONCLUSIONS: Our results set forth the importance of skin DC in cancer immunotherapy, and demonstrates that restoring DC function is key to enhancing tumor immunogenicity and subsequently responsiveness to checkpoint blockade therapy

Investigation on the association of cardiovascular markers with severity of chronic pyelonephritis

Chronic kidney disease (CKD) is an established independent risk factor for cardiovascular disease (CVD) and is caused by chronic pyelonephritis (CP). This study aimed to investigate the effect of the association of cardiovascular markers with the course of CP on the comorbidity of CP with ischemic heart disease. The study participants included 125 patients with CP without symptoms of urinary tract obstruction who were divided into three group

Mechanisms of Development of Heart Failure in Chronic Kidney Disease

The study of scientific data over the past 5 years has shown that cardiorenal syndrome, which has a complex and multifactorial pathophysiology, is a clinical problem. Modern pharmacological methods of treatment are effective, but insufficient to satisfactorily influence or mitigate the progression of cardiorenal syndrome, therefore, the discovery of new drugs and new therapeutic strategies for cardiorenal syndrome is a high priority tas